African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 15 May 2012; 11(39)

May 2012

Forest biomass resources and utilization in China

  Under the context of climate change, persistent high oil prices and rapidly growing dependence on imported oil prompt China to pay much more attention to biofuels that provide environmental benefits besides fuel. China has rich biodiversity with 30 thousand high plant species and 154 kinds of energy trees could produce seeds containing more than 40% of oil, with total production of the seeds...

Author(s): Jianfeng ZHANG, Guangcai CHEN, Qixing SUN, Zebo LI, Ying WANG

May 2012

Co-extraction of high-quality RNA and DNA from rubber tree (Hevea brasiliensis)

  High-quality nucleic acids are the basic requirement for performing genomic research. A reliable and efficient method was developed for co-extracting high-quality DNA and RNA from rubber tree (Hevea brasiliensis) in this study. Polyethylene glycol (PEG) and cetyltrimethylammonium bromide (CTAB) extraction buffer with high concentrations of polyvinylpyrrolidone (PVP) and b-mercaptoethanol was used in...

Author(s): Zewei An,#, Qitong Wang,#, Yanshi Hu, Yanhong Zhao, Yacao Li, Han Cheng, Huasun Huang

May 2012

Molecular characterization and novel genetic variability in leptin (obese) gene of mithun (Bos frontalis)

  The present study was undertaken with the objectives of sequencing, characterization and single nucleotide polymorphisms (SNPs) identification of mithun leptin gene. The mithun leptin gene (3420 bp) was sequenced, compared with other species and phylogenetic tree were constructed. Single-strand conformation polymorphism (SSCP) showed three patterns in both exon-2 and exon-3 of the gene. Nucleotide sequences...

Author(s): P. P. Dubey,#, D. S. Gour#, Prashant Singh, Anubhav Jain, Amit Kumar, S. C. Gupta and Dinesh Kumar

May 2012

Synthesis, characterization and antibacterial screening of 2,4-diaminopyrimidine pyrimethamine and trimethoprim silver complexes

  Air stable silver Ag(I) complexes of pyrimethamine and trimethoprim drugs have been synthesized and characterized by elemental analysis, Fourier transform infrared (FTIR) and ultraviolet visible (UV-Vis) spectroscopy, and conductivity measurement. The metal complexes formed a three and four coordinate geometry with the ligands acting as a monodentate molecule bonding to the silver ion in each case through the...

Author(s): Omoruyi G. Idemudia, Peter A. Ajibade and Anthony I. Okoh

May 2012

Compositional assessment of carotenoid-biofortified rice using substantial equivalence

  One important aspect in assessing the safety of genetically modified (GM) crops for human consumption is characterizing their nutrient composition. A β-carotene-biofortified rice was generated by inserting phytoene synthase (Psy) and carotene desaturase (Crtl) genes isolated from Capsicum and Pantoea into the genome of a conventional variety of rice (Nakdongbyeo). Nutrients...

Author(s): Jae Kwang Kim, Soo-Yun Park, Sun-Hwa Ha, Si Myung Lee, Sun Hyung Lim, Hyo Jin Kim, Hyeon-Seok Ko, Sung-Dug Oh, Jong-Sug Park and Seok-Cheol Suh

May 2012

Effect of colchicine on mitotic polyploidization and morphological characteristics of Phlox drummondi

  The present work was undertaken to observe the response of Phlox drummondi to colchicine treatment. The survival rate and germination percentage is severely affected by various treatments of colchicine. In this investigation, doses related effects of the ploidy treatments on quantitative traits were noticed. Result indicates reduction in plant height, number of leaves per branch, but increase in...

Author(s): A. K. Tiwari and S. K. Mishra

May 2012

Comparative evaluation of organic wastes for improving maize growth and NPK content

  Plant residues being organic in nature are rich source of macro and micronutrients and can be recycled to prevent their disposal in the environment, thus sustaining the balance between economic development and environmental protection. The potential of three agricultural waste composts, farmyard manure (FYM), banana waste (BW) and pressmud (PM) was tested in a pot experiment growing maize. The results of the...

Author(s): M. Memon, K. S. Memon, S. Mirani and G. M. Jamro

May 2012

Effect of rock fragments on macropores and water effluent in a forest soil in the stony mountains of the Loess Plateau, China

  Rock fragments exert important effects on soil water movement and macropores.However,they are not well-studied in forest hydrological processes. The results show that the steday effluent is markedly affected by the radius and density of macropores, especially the density of macropores with radii>1.4mm make up only 6.86% of the total,they contribute 67.4% to the variance of steady effluent rates. The area...

Author(s): Zhongjie Shi, Lihong Xu, Yanhui Wang, Xiaohui Yang, Zhiqing Jia, Hao Guo, Wei Xiong and Pengtao Yu

May 2012

Evaluation of rice genotypes to salt stress in different growth stages via phenotypic and random amplified polymorphic DNA (RAPD) marker assisted selection

  Soil salinity represents an increasing threat to rice production. The success of salt tolerance breeding programs employing traditional screening and selection has been limited in the past decades. 40 rice genotypes were screened in saline soil of electrical conductivity (EC) of 4, 8 and 12 ds/m in vegetative growth stage in 2009. Tolerant genotypes were tested in young seedling stage in hydroponic...

Author(s): Shahrbano Mirdar Mansuri, Nadali Babaeian Jelodar and Nadali Bagheri

May 2012

The effect of polythene colour container and three spawn rates on production of Pleurotus ostreatus mushroom

  To study the effect of polythene colour container and spawn rates on mushroom (Pleurotus ostreatus), experiment was carried out to investigate the cultivation on barely straws substrate. This cultivation system can improve the economic status of the farmer. Field experiment was conducted at College of Agriculture, University of Zabol during 2011 growing season. Experiment was carried out as factorial and...

Author(s): Mehdi Dahmardeh

May 2012

Chemical composition of Chinese palm fruit and chemical properties of the oil extracts

  The proximate composition, mineral concentration of fleshy mesocarp, palm meat (PM) and palm kernel (PK) of oil palm fruit (Elaeis guineensis S.L.Dura) produced in Hainan, China were investigated. The fatty acid composition, chemical properties and minor constituents of palm oil (PO) and palm kernel oil (PKO) were also studied. The crude fat of PM and PK were 68.09±3.57% and 49.36±2.61%,...

Author(s): Rui Li, Qiuyu Xia, Minmin Tang, Songlin Zhao, Weijun Chen, Xintao Leiand Xinpeng Bai

May 2012

Effects of two different soil amendments on the biocontrol efficacy of biological control agents (BCA) against Ralstonia wilt on ginger

  In this research, we developed an integrated approach to control Ralstonia wilt on ginger. Two organic composts (maize powder and soybean residue) were combined with one combination (the aqueous solution of Bacillus subtilis and Bacillus cereus) and two single (B. subtilis 1JN2 and Bacillus megaterium B1301) biological control agents (BCA) which together showed significant...

Author(s): Wei Yang, Hongxia Liu, Yunpeng Wang, Yuming Luo, Hetong Yang and Jianhua Guo

May 2012

Independent behavior of bacterial laccases to inducers and metal ions during production and activity

  Laccase, a blue copper oxidase, is an enzyme that is involved in the oxidation of aromatic compounds which prove otherwise difficult to degrade in the environment. The substrates of laccase are xenobiotics and synthetic dyes. The isolation of bacterial strains was investigated for laccase production and its activity. The medium for production was a high nitrogen medium containing aromatic compounds as...

Author(s): Wiyada Mongkolthanaruk, Sasiprapa Tongbopit and Artidtaya Bhoonobtong

May 2012

The bioaccumulation and toxicity induced by gold nanoparticles in rats in vivo can be detected by ultraviolet-visible (UV-visible) spectroscopy

  It is essential to characterize the bioaccumulation and toxicity of gold nanoparticles (GNPs) in blood prior to using them in drug delivery, diagnostics, and treatment. The aim of the present study was to evaluate the blood absorbance spectra after intraperitoneal administration of 50 µl of 10, 20, and 50 nm GNPs in rat for periods of 3 and 7 days to assess their bioaccumulation and toxic effects....

Author(s): Mohamed Anwar K. Abdelhalim

May 2012

Cobalt removal from wastewater using pine sawdust

  Agricultural wastes can cause environmental problems if not well managed, but there is a lot of potential to use these wastes as raw material in other processes. In this investigation, pine sawdust was evaluated as an adsorbent in the treatment of wastewater containing cobalt ions. A two-level three-factor full-factorial experimental design with centre points was used to study the interactive effect of the...

Author(s): Evans T. Musapatika, Ruella Singh, Krishnie Moodley, Charles Nzila, Maurice S. Onyango and Aoyi Ochieng

May 2012

Antimicrobial properties of probiotic bacteria from various sources

  The probiotic potentials of lactic acid bacteria species isolated from various food sources (nono, ugba, ogiri, kunun-zaki and ogi) were studied. The predominant species among the isolated strains were Lactobacillus bulgaricus, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus brevis, Streptococcus thermophilus Leuconostoc mesenteroides and Pediococcus cerevisiae. They produced...

Author(s): Okereke, H. C., Achi, O. K., Ekwenye, U. N. and Orji, F. A.

May 2012

Effect of the purification of antidermatophytic proteins from Nigella sativa on four zoophilic species

  The antidermatophytic activities of proteins which are extracted from four plant species (Carum carvi, Cymbopogon citratus, Moringa oleifera, and Nigella sativa) on four zoophilic dermatophytes (Microsporum canis, Microsporum equinum, Trichophyton mentagrophytes, and Trichophyton verrucosum) were evaluated in this study. The crude proteins of N. sativa had the...

Author(s): Neveen S. Geweely, and Saleha Y. M. alakilli

May 2012

Physicochemical and microbiological characterization of linolenic acid-rich oils from seeds of two tropical plants: Corchorus olitorius L. and Hibiscus sabdariffa L.

  his work was carried out to determine the potential applications of Corchorus olitorius and Hibiscus sabdariffa oil seeds by investigating their physicochemical and microbiological characteristics. Physicochemical parameters of the extracted oils were respectively as follow: refractive index (1.46 and 1.45), acid value (3.74 ± 1.62 and 2.80 mg KOH/g), peroxide value (6.67 ±...

Author(s): Lessoy Zoué, Micael Bédikou, Betty Faulet, Jean Gonnety and Sébastien Niamké

May 2012

Bacteriological study of food in the Pakistan’s peri-urban areas of Rawalpindi and Islamabad

  In order to ascertain the food quality for human consumption the bacteriological analysis was carried out in a prospective study in collaboration with National Institute of Health, Islamabad. The surveillance work was conducted during the months of July to August peak summer season to evaluate the objective parameters for assessment of the bacteriological and hygienic status of food being consumed in the...

Author(s): Nusrat Yasin , Jafar Khan, Noureen Shah, Zia ul islam, Rashid Azim Khan, Noor us Saba

May 2012

Monitoring paneer for Listeria monocytogenes- A high risk food pathogen by multiplex PCR

  A multiplex polymerase chain reaction (PCR) assay was developed and applied to spiked and natural paneer samples to detect Listeria monocytogenes, a high risk food pathogen. The sensitivity of the assay on L. monocytogenes spiked paneer samples was 104 cells prior to enrichment, was improved to 103 cells after 4 h enrichment and one to 10 cells after 6 h enrichment as indicated by the...

Author(s): Ashwani Kumar, Sunita Grover and Virender Kumar Batish

May 2012

Antioxidative, anticholinesterase and antityrosinase activities of the red alga Grateloupia lancifolia extracts

  The solvent extracts of a red alga Grateloupia lancifolia were used to evaluate biofunctional activities. The diethyl ether extract showed the highest total phenolic content and biological activities. The IC50 value of the diethyl ether extract on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was 113.8 g/ml. In the reducing power test, the half maximal effective...

Author(s): Han Nguyen,  and Sang Moo Kim

May 2012

Antiviral and antitumor activities of the protein fractions from the larvae of the housefly, Musca domestica

  In this study, we present the extraction and purification of protein fractions from the larvae of the housefly, Musca domestica. The bioactivities of the protein fractions were indicated by pseudorabies virus (PRV) and human lung cancer cell line A 549. The crude protein fractions had no toxicity to chick embryo fibroblast-like (CEF) cells but effective to PRV at the concentration of 100 µg/ml...

Author(s): Yong Wang#, Di Lu#, Yao Zhao, Chaoliang Lei and Fen Zhu

May 2012

An efficient and rapid method for protein detection with an example of sulfide-quinone reductase expressed in Escherichia coli

  Conventional Coomassie brilliant blue (CBB) staining is widely used in protein detection as it is inexpensive and reproducible. Conversely, it is also very time-consuming and cumbersome. In this study, CBB(R-250)-microwaved-water (CMW) was used to detect the sulfide-quinone reductase (SQR) protein expressed inEsherichia coli by staining and destaining in under 30 min. The CMW method has similar...

Author(s): Yan-fen He#, Ai-ling Zhang# and Jia-qi Li

May 2012

Expression of human β-defensin-1 in recombinant Escherichia coli and analysis of its antimicrobial spectrum

  Escherichia coli BL21(DE3) was transformed with a pHCD1 plasmid harboring the human β-defensin-1 (hBD1) gene fused in frame behind a disulfide bond isomerase (DsbC), a His-tag, and an enterokinase cleavage site. After induction, the DsbC-hBD1 was expressed as a ~36 kDa soluble fusion protein in recombinant E. coli, which also inhibited host cell growth. After cell disruption, the soluble...

Author(s): Myung-Ji Seo#, Hak-Jong Choi#, Jung-Choul Lee, Yu-Ryang Pyun and Hoon Park

May 2012

Tissue-specific alternative splicing and expression of ATP1B2 gene

  The Na+-K+-ATPase is an essential transport enzyme expressed in all animal tissues, where it generates ion gradients to maintain membrane potential and drive the transport of other solutes. It also balances metabolism and body temperature. In this study, the characterization of three novel bovine ATP1B2 splice variants, designated as ATP1B2-AS1, ATP1B2-AS2, and ATP1B2-AS3, is discussed. All three novel splice...

Author(s): Zeying Wang, Jinming Huang, Jifeng Zhong and Genlin Wang

May 2012

Antifungal activity of methanolic extracts of four Algerian marine algae species

  Since ancient times antimicrobial properties of seaweeds have been recognized. In this study, antifungal activity of four species of marine algae of Bejaia coast (Algeria) was explored. This activity was evaluated by agar diffusion method. The minimum inhibitory concentrations were also determined for all the strains. All the extracts used in this study exhibited antifungal activity. The highest...

Author(s): SAIDANI K., BEDJOU F., BENABDESSELAM F. and TOUATI N.

May 2012

Evaluation of economic traits in progenies of Nigerian heavy ecotype chicken as genetic material for development of rural poultry production

  Genetic evaluation of egg production and biometrical traits of the first filial generation of the Nigerian heavy ecotype chicken was carried out. Heritability and genetic correlations between the traits were estimated using the mixed model least squares and maximum likelihood computer programme of Harvey. Results show an average hen day production of 58.91% on the first month and 52.69% on the fourth month of...

Author(s): Agu, C. I., Ndofor-Foleng, H. M. and Nwosu, C. C.

May 2012

Comparative genomic in situ hybridization analysis on the chromosomes of five grass species with rice genomic DNA probe

  Comparative genomic in situ hybridization (cGISH) with biotin-labeled rice genomic DNA to the chromosomes of Zea mays, Hordeum vulgare, Sorghum bicolor, Setaria italic and Secale cereale were conducted to analyze genomic homology between rice and other grass (Gramineae) speices. At 75% stringency, the rice DNA probe generated large numbers of signals dispersedly...

Author(s): Chao-Wen She, Yun-Chun Song and Xiang-Hui Jiang,

May 2012

Effect of feeding inulin oligosaccharides on cecum bacteria, egg quality and egg production in laying hens

  Inulin is reported to improve the egg quality and production of laying hens. In the present study, we investigated the dietary effects of microcapsulated inulin oligosaccharide (INO) which is manufactured from Jerusalem artichoke (Helianthus tuberosus L.) on the cecum bacteria, egg quality and production of laying hens. 400 laying hens were randomly allocated to one of the following four treatment...

Author(s): Park, Sang-Oh and Park, Byung-Sung