African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 16 August, 2012; 11(66)

August 2012

Cloning and semi-quantitative expression of endochitinase (ech42) gene from Trichoderma spp.

  Species of Trichoderma such as T. harzianum, T. viride and T. atroviride are some of the most potent antagonistic fungi, and have been used as plant growth promoters in developed countries. Endochitinase (ech42) gene which is involved in mycoparasitism, was isolated from Trichoderma spp. taken from hot-arid soils of Rajasthan, cloned, sequenced and its expression...

Author(s):   Pratibha Sharma, Saravanan, K., Ramesh, R., Vignesh Kumar P, Dinesh Singh, Manika Sharma, Monica S. Henry and Swati Deep.  

August 2012

Effects of electron beam radiation on trait mutation in azuki bean (Vigna angularisi)

  Dry seeds of azuki bean (Vigna angularisi), Jingnong 6 and Hebei 801 varieties were irradiated by electron beam of 100, 300, 600, 700 and 900 Gy, respectively. Mutations of leaf shape and color, seed size and shape, trailing, more branching, dwarfing, early or late flowering time and high yield were created in...

Author(s):   Wan-Xia Luo#, Yi-Song Li#,  Bao-Mei Wu, Yu-Er Tian, Bo Zhao, Li Zhang, Kai Yang and Ping Wan      

August 2012

Identification of differentially expressed sequences in bud differentiation of oriental hybrid lily cultivar ‘Sorbonne’ via suppression subtractive hybridization

  The developmental process of lily flower bud differentiation has been studied in morphology thoroughly, but the mechanism in molecular biology is still ambiguous and few studies on genetic expression have been carried out. Little is known about the physiological responses of flower bud differentiation in Oriental hybrid lily ‘Sorbonne’ (Lilium spp.) during the stages of...

Author(s):   Xiaohua Liu, Jie Huang, Yingjie Zhang and Yingmin Lu    

August 2012

Molecular characterization of genetic diversity in some durum wheat (Triticum durum Desf.) in Palestine

  Random amplified polymorphic DNA (RAPD) analysis was conducted to estimate the genetic diversity and relatedness among 10 wheat cultivars and landraces. Five out of 70 decamer random primers showed distinctly polymorphic bands when used to amplify genomic DNA. The primers yielded a total of 157 RAPD markers of which 150 were considered as polymorphic. Each primer produced 18 to 37 polymorphic...

Author(s):   Heba Al-Fares and Hassan Abu-Qaoud    

August 2012

Cost-effective nutrient sources for tissue culture of cassava (Manihot esculenta Crantz)

  Application of tissue culture technology is constrained by high costs making seedlings unaffordable. The objective of this study was to evaluate the possibility of using locally available fertilizers as alternative nutrient sources for cassava micropropagation. A Low Cost Medium (LCM) whereby the conventional sources of four Murashige and Skoog (MS) macronutrients had been replaced with locally available...

Author(s):   Ogero K. O., Gitonga N. M., Mwangi M., Ombori O. and Ngugi M.  

August 2012

Large scale production of antitumor cucurbitacins from Ecballium Elaterium using bioreactor

  Bioreactor plays a vital role in the commercial production of secondary metabolites and pharmaceuticals from plant cells. Many physical factors, like the mixing intensity, shear stress and operation conditions were optimized as a first step in scaling up process. Qualitative and quantitative determination of cucurbitacins E and I in the extract were carried out using high-performance liquid chromatography...

Author(s):   Mahmoud M. Saker, Mai M. Farid, Abdelgawad A. Fahmi, Sahar A. El-Mekkawy, Hussein S.Taha and Ahmed I. Amin  

August 2012

Evaluation of the expression of internal control transcripts by real-time RT-PCR analysis during tomato flower abscission

  Many investigations of the abscission mechanisms in plants are based on gene expression studies. Quantitative real-time RT-PCR (qRT-PCR) is widely and increasingly used for mRNA quantification, and the results are typically referenced to an internal control gene to avoid bias. We investigated the suitability of twelve tomato(Lycopersicon esculentum Mill. var. Liaoyuanduoli) housekeeping genes during...

Author(s):   Song Gao, Tao Xu, Mingfang Qi, Yufeng Liu, Hong Li, Shuangshuang Lv, Jinhong Li and Tianlai Li      

August 2012

Expression analysis of banana MaECHI1 during fruit ripening with different treatments

  The main function of endochitinase is believed to be pathogenesis related protein. However, more and more scientists reported the roles of endochitinase in plant growth and development. In order to investigate the role of endochitinase in postharvest banana fruit ripening, an endochitinase gene known as MaECHI1 had been isolated from a suppression subtractive hybridization (SSH) complementary...

Author(s):   Ju-Hua Liu#, Jing Zhang#, Bi-Yu Xu, Jian-Bin Zhang, Cai-Hong Jia, Jia-Shui Wang and Zhi-Qiang Jin,    

August 2012

Effect of 1-methylcyclopropene and vacuum cooling treatments on post harvest quality of Salicornia bigelovii Torr.

  Salicornia bigelovii Torr. is a potential human food supplement that can be cultivated in subtropical salt marsh with seawater irrigation. However, this plant is not only perishable but also highly seasonal. The objectives of this study were to examine if the prestorage treatments with several concentrations of 1-methylcyclopropene (1-MCP) from 0 to 2.5 mL L-1, and vacuum cooling would...

Author(s):   Dong-He Lu, Min Zhang, Shao-Jin Wang, Chengpei Zhu and Xiang Zhou        

August 2012

Accelerated ripening of Kashar cheese with encapsulated protease

  In this study, protease enzymes were encapsulated in k-carragenan, gellan andsodium alginate using emulsion and extrusion techniques and were then added in cheese milk together with rennet. The effects of the encapsulating material and ripening period on the chemical, textural and sensory characteristics of Kashar cheese were investigated. The study demonstrated that sodium alginate, gellan...

Author(s):   M. S. Akin  

August 2012

Effects of electromagnetic field of 33 and 275 kV influences on physiological, biochemical and antioxidant system changes of leaf mustard (Brassica chinensis)

    The effects of electromagnetic fields (EMF) from 33 and 275 kV high voltage transmission line on biochemical and antioxidant system changes in mustard leaf (Brassica chinensis) were investigated under field condition. Mustard leaves were exposed to EMF from power lines at distances of 0, 3, 6, 9, 10, 12, 15, 18, 20, 21, 30, 40, 50 and 60 m away from the 33 kV power line and at 0, 10, 20, 30, 40,...

Author(s):   Maziah, M., Ooi, B. B., Tengku, M. and Sreeramanan, S.,      

August 2012

Overwintering physiology of the rice stem borer larvae, Chilo suppressalis Walker (Lepidoptera: Pyralidae): Roles of glycerol, amino acids, low-molecular weight carbohydrates and antioxidant enzymes

  The rice stem borer, Chilo suppressalis (Walker), is a major rice pest around the world. A strong ability of the rice stem borer to adapt/resist cold temperature (cold hardiness) contributes to its survival through winter. However, the physiological mechanism of its cold hardiness is poorly understood. In this study, we determined the supercooling points (SCPs), the contents of amino acids and...

Author(s):   QIANG Cheng-kui, DU Yu-zhou, QIN Yue-hua, YU Lin-ya, ZHOU Bao-ya, FENG Wu-jian and WANG Song-song    

August 2012

Transcriptional peroxisome proliferator-activated receptor γ coactivator-1 (PGC-1α) regulates transformation of muscle fiber type in Schizothorax prenanti

  Peroxisome proliferator-activated receptor γ coactivator (PGC)-1É‘, a well-known member of PGC-1 transcriptional coactivator’s family, plays a key role in various metabolic pathways. Here, we investigated the role of PGC-1É‘ in the transformation of muscle fiber type in Schizothorax prenanti. The expression of PGC-1É‘ was induced inS. prenanti muscles following fasting....

Author(s):   Yaqiu Lin#, Ruiwen Li#, Yucai Zheng , Runfeng Zhang, Juchun Lin and Xiang Qiu    

August 2012

Effect of acrylic acid on the properties of polyvinylpyrrolidone hydrogel prepared by the application of gamma radiation

  Hydrogels based on polyvinylpyrrolidone (PVP) networks grafted with acrylic acid (AAc) was prepared by using γ-rays from a Co-60 source at room temperature. The parameters like effect of radiation dose and concentration of AAc were studied. The properties such as gel content, swelling behavior and thermal stability were also evaluated. The result indicated that gel content of hydrogel...

Author(s):   Dafader N. C., Tahmina Akter, Haque M. E., Swapna S. P., Sadia Islam and Huq D.  

August 2012

Preparation and characterization of water-absorbing composite membrane for medical applications

  The present work introduces a novel method for the formation of water absorbing composite membrane. The prepared composite is based on chitosan, carboxymethyl cellulose (CMC), and Montmorillonite. Prepared composite membrane exhibits high water absorbing and holding capacity with increasing clay content. The water absorbency was studied as a function of the hydrogel composition and the medium pH....

Author(s):   Taher A. Salah El-Din, Ahmed A. Elzatahry,Sultan S. Aldeyab and Elbadawy A. Kamoun