African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 17 July 2012; 11(57)

July 2012

Karyoevolutionary and karyosystematic considerations on Schizothorax curvifrons and Schizothorax niger (Teleostei: Cyprinidae): Important hill-stream food fishes of Kashmir Himalaya

Cytogenetic studies have helped in clarifying the problem of disagreement amongst taxonomists on the identity of a given species. Cytogenetic studies were performed on two fishes of the genus Schizothorax viz. Schizothorax curvifrons Heckel andSchizothorax niger Heckel (Cyprinidae: Schizothoracinae) obtained from Sindh Stream and Dal Lake Srinagar Kashmir, respectively. These fishes are...

Author(s): Farooq Ahmad Ganai, Sabzar Ahmad Dar, A.R. Yousuf, N.K. Tripathi and Samee Wani

July 2012

Construction of retrovirus vector taking MDR1/ACBC1 and its transfection into human placenta derived mesenchymal stem cells

  In the study, we used both the methods of perfusion and density gradient centrifugation to isolate and purify mesenchymal stem cells (MSCS) from placenta tissue, and constructed a retroviral vector with multiple drug resistant genes, and the green fluorescent protein (GFP) has been used as an indicative mark. The 293T cell was transfected by the retroviral vector PMX-flag-MDR1-GFP together with its peripheral...

Author(s):   Wang Bowei, Bi Ming, Gao Shang, Liu Zhihui and Zhou Yanmin        

July 2012

Recovery of antioxidant gene expression in sacred lotus (Nelumbo nucifera Gaertn.) embryonic axes enhances tolerance to extreme high temperature

Sacred lotus (Nelumbo nucifera Gaertn.) seed is long-living and have various stress-resistance characteristics. We investigated the protecting mechanisms of lotus seeds against extreme high temperature by comparison of expression patterns of antioxidant genes in embryonic axes between exposure and non-exposure to extreme high temperature. It was shown that viability of seeds did not severely decline after exposure...

Author(s): Yin Li, Yishun Zhang, Shaowei Wei, Li Liu and Yunfeng Chen

July 2012

Stable Agrobacterium-mediated transformation of the halophytic Leymus chinensis (Trin.)

In this study, an efficient procedure for stable Agrobacterium-mediated transformation of Leymus chinensis (Trin.) was established. Agrobacterium tumefaciens strain EHA105, harboring a binary vector pCAMBIA2300, was used for transformation, along with a sweet potato 2-cysteine peroxiredoxin (2-Cys Prx) gene under the control of the stress-inducible sweet potato anionic peroxidase 2 (SWPA2)...

Author(s): Yan-Lin Sun, and Soon-Kwan Hong,

July 2012

Studies on some morphological characters and yield attributes of synthetic Brassica hexaploids and their parents

Allopolyploidy plays an important role in plant evolution and confers better advantages on crop growth and breeding compared to low ploidy levels. The present investigation aimed to study the synthetic hexaploid Brassica (AABBCC genome) developed fromBrassica cainata (BBCC genome) and Brassica rapa (AA genome) compared to their parental genotypes considering some morphological...

Author(s): M. A. Malek, L. Rahman, L. Hassan, M. I. Uddin, M. Y. Rafii and M. R. Ismail

July 2012

Sequence analysis of putative swrW gene required for surfactant serrawettin W1 production from Serratia marcescens

Serratia marcescens produces biosurfactant serrawettin, essential for its population migration behavior. Serrawettin W1 was revealed to be an antibiotic serratamolide that makes it significant for deoxyribonucleic acid (DNA) and protein sequence analysis. Four nucleotide and amino-acid sequences from local strains analyzed through bioinformatics showed high confidence prediction of serrawettin. Database comparison...

Author(s): Monabel May N. Apao, Franco G. Teves and Ma. Reina Suzette B. Madamba

July 2012

Optimization of growth regulators in organogenesis of Bletia purpurea (Lam.) using response surface design and genetic evaluation

This paper reports the optimal concentrations of indol acetic acid (IAA) and benzylaminopurine to stimulate morphogenetic induction Bletia purpurea (Lam.) and the application of random amplification of polymorphic deoxyribonucleic acid (DNA) (RAPD) for genetic evaluation of micropropagated plantlets. Organogenesis was induced from B. purpurea (Lam.) explant pseudobulbs in Murashige and Skoog...

Author(s): Yah-Chulim Julia Esther, Rodríguez-Buenfil Ingrid Mayanin, Reyes-Escogido María de Lourdes and López-Puc Guadalupe

July 2012

Redox-mediated polymerization and removal of benzidine from model wastewater catalyzed by immobilized peroxidase

Peroxidase from Momordica charantia was highly effective, active and stable for the oxidation of benzidine from model wastewater. There was no oxidative polymerization of benzidine without any redox mediator. Various experimental parameters were standardized for the maximum oxidation of benzidine by peroxidase. The maximum oxidation of this pollutant was observed in the presence of 0.05 mM phenol, 0.75 mM...

Author(s): Zoheb Karim, Qayyum Husain and Rohana Adnan

July 2012

Isolation and identification of fungal species from dried date palm (Phoenix dactylifera) fruits sold in Maiduguri metropolis

A total of 360 dried date palm (Phoenix dactylifera) fruits were collected from hawkers, shops and market places within Maiduguri metropolis for the detection of the presence of fungal species. Investigation was based on cultural, microscopically and biochemical tests. Of the 327 (90.83%) fungal isolates recovered on Sabouraud dextrose agar (SDA), Aspergillus niger had the highest percentage of occurrence...

Author(s): Colman, S., Spencer, T. H. I., Ghamba, P. E. and Colman, E.

July 2012

Effect of medium composition and ultrasonication on xylanase production by Trichoderma harzianum MTCC 4358 on novel substrate

Sunflower sludge was used as the sole carbon source for xylanase production, an important industrial enzyme used in pulp and paper industry, by the fungi Trichoderma harzianum MTCC 4358. Sunflower sludge was subjected to alkaline pretreatment which showed higher enzyme activity than the substrate used without pretreatment. The effect and significance of the medium components were studied using the...

Author(s): Punniavan Sakthiselvan, Balakrishnan Naveena and Nagarajan Partha

July 2012

Effect of medium composition and ultrasonication on xylanase production by Trichoderma harzianum MTCC 4358 on novel substrate

  Sunflower sludge was used as the sole carbon source for xylanase production, an important industrial enzyme used in pulp and paper industry, by the fungi Trichoderma harzianum MTCC 4358. Sunflower sludge was subjected to alkaline pretreatment which showed higher enzyme activity than the substrate used without pretreatment. The effect and significance of the medium components were studied...

Author(s):   Punniavan Sakthiselvan, Balakrishnan Naveena and Nagarajan Partha        

July 2012

Mutation breeding of Bacillus subtilis YTB4 with high yield of multienzyme complex

Helium-neon (He-Ne) laser irradiation is a highly efficient mutation breeding technology and is widely applied to various fields of biological science. Using Bacillus subtilis YTB4 with high yield of multienzyme complex as original strain, mutation breeding was carried out by He-Ne laser irradiation in this study. Based on the study of the strains’ survival rate and positive mutation...

Author(s): Peng Song, Liang Huang and Xiu-pu Guo

July 2012

Effects of probiotic (live and inactive Saccharomyces cerevisiae) on meat and intestinal microbial properties of Japanese quails

The present work evaluated the effect of probiotic (live and inactive Saccharomyces cerevisiae) on meat and intestinal microbial properties of Japanese quails. Twenty-four (24) 1-day-old Japanese quails were obtained from a commercial hatchery. The birds were randomly divided into 2 groups. The dietary treatments were: 1) basal diet (control), 2) basal diet plus 0.1% live S....

Author(s): Afshin Javadi, Hamid Mirzaei, Saeid Safarmashaei and Sina Vahdatpour

July 2012

Oil characteristics and fatty acid profile of seeds from three varieties of date palm (Phoenix dactylifera) cultivars in Bushehr-Iran

Studies were conducted on properties of seed oils extracted from three date palm (Phoenix dactylifera) varieties Shahabi, Shekar and Kabkab, grown in Bushehr-Iran.The primary objective of this study was to extract seed-oil from the three native date varieties and then analyzed their fatty acids (FAs). Also the chemical parameters of PV, AV, p-anisidin, and Totox values of oils, as indicators of the...

Author(s): Akbari, M., Razavizadeh, R., Mohebbi, G. H. and Barmak, A.

July 2012

Protozoidal activities of Eucalyptus cammeldulensis, Dalbergia sissoo and Acacia arabica woods and their different parts on the entozoic flagellates of Heterotermes indicola and Coptotermes heimi

Different parts of three woods of Eucalyptus cammeldulensis, Dalbergia sissoo and Acacia arabica were analyzed for their toxicity potentials against two species of termites (Heterotermis indicola and Coptotermis heimi). Termite workers were allowed to feed on 2 g complete wood powder of plant species and their parts, including; bark, sapwood and heartwood. Samples of flagellates were collected after each 24 h from the...

Author(s): Naveeda Akhtar Qureshi, Muhammad Zahid Qureshi, Naeem Ali, Muhammad Athar, and Aziz Ullah

July 2012

Expression and sequence characterization of growth hormone binding protein of Nili-Ravi buffaloes (Bubalus bubalis)

  The growth hormone binding protein (GHBP) was isolated from the liver of Nili-Ravibuffaloes (Bubalus bubalis), reverse transcriptase-polymerase chain reaction (RT-PCR) amplified and sequence characterized. RT-PCR analysis demonstrated high degree sequence identities (97.3 to 99.6%) of BbGHBP cDNA with Bos taurus, Ovis aries and Capra hircus. An expression plasmid was...

Author(s):   Roquyya Gul, Saima Sadaf and Muhammad Waheed Akhtar,        

July 2012

Potential nutritive value of some forage species used as ruminants feed in Iran

A study was conducted to determine rumen degradability (in sacco) of dry matter andin vitro gas production of the most important forage species grown in Iran, to compare forage species according to calculated degradation and in vitro gas production parameters, and to establish prediction equations for relative feed value (RFV) from gas production parameters. Thus, six forage species consisting...

Author(s): Kazemi, M., Tahmasbi, A. M., Naserian, A. A., Valizadeh, R. and Moheghi, M. M

July 2012

Analysis of pig serum proteins based on shotgun liquid chromatography-tandem mass spectrometry

Recent advances in proteomics technologies have opened up significant opportunities for future applications. We used shotgun liquid chromatography, coupled with tandem mass spectrometry (LC-MS/MS) to determine the proteome profile of healthy pig serum. Samples of venous blood were collected and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis separation and in-gel trypsin digestion. The peptides...

Author(s): Keshan Zhang, Yongjie Liu, Youjun Shang, Haixue Zheng, Jianhong Guo, Hong Tian, Ye Jin, Jijun He and Xiangtao Liu

July 2012

Effect of low protein diets and lysine supplementation on growth performance and carcass characteristics of growing pigs

The present study was to assess the effect of feeding low protein diet with or without supplemental lysine to meet NRC (1998) requirement on growth performance, carcass trait, meat composition, and meat quality of pigs. An experiment of 126 days was conducted on 21 crossbred Landrace pigs (average weight 11.72 ± 0.57 kg, average age 7.59 ± 0.14 weeks). Animals were randomly assigned to three dietary...

Author(s): A. Kumar, R. Bhar, A. B. Mandal and S. K. Mendiratta

July 2012

Study on the alteration of bubaline blood biochemical composition owing to slaughter

Bubaline blood biochemistry as affected by slaughter was the agenda for this work. Blood samples were collected from 30 buffaloes from abattoirs before and at slaughter. After biochemical and statistical analysis (mean was compared with t-test), it was observed that the albumin, lactate dehydrogenase and creatine kinase increased (p < 0.05), while globulin, urea nitrogen and creatinine decreased (p < 0.05)...

Author(s): Anirban Guha, Ruby Guha and Sandeep Gera

July 2012

Antimicrobial residues screening in pigs and goats slaughtered in Nsukka Municipal abattoir, Southeast Nigeria

Antimicrobial residue in animal food products is an important index of food safety. Drug residues could result from chemotherapeutic or chemoprophylactic use of drugs in food animals. This occurrence of residue in animal food products has received enormous worldwide attention from some local, international and public health agencies. Samples of tissues/organs from pigs and goats slaughtered at the Nsukka Municipal...

Author(s): Ekene V. Ezenduka and Chinyere Ugwumba