December 2013
Role of alternative oxidase in postharvest stress of fruit and vegetables: Chilling injury
Chilling injury (CI) imposes a limitation for extending storage of fruit and vegetables. Chilling injury can result from oxidative stress caused by reactive oxygen species (ROS). Alternative oxidase (AOX) as a ROS avoidance genes play pivotal role in defense mechanism against chilling injury derived oxidative stress. Postharvest treatment of fruit and vegetables through methyl salicylate, methyl jasmonate and storage in...
December 2013
Comparison of gene expression proï¬les in Bacillus megaterium treated tobacco leaves using microarray
The MP agent, prepared from Bacillus megaterium isolated from the soil near tobacco fields, can improve metabolic products, and hence the aroma, of tobacco (Nicotiana tabacum) leaf. To explore genes regulating metabolic responses in tobacco leaf, we used microarrays to analyze differentially expressed genes in tobacco leaves subjected to various treatments. The expressed genes were classified into six groups based on...
December 2013
Genetic diversity studies in common bean (Phaseolus vulgaris L.) using molecular markers
Molecular characterization of thirteen common bean genotypes was done with random amplified polymorphic DNA (RAPD) markers. Initially, 15 primers were screened out of which only seven were selected which generated a total of 65 amplification products out of which 63 bands (96.62%) were polymorphic indicating fair amount of polymorphism. The genotypes shared 43% genetic similarity among themselves. Cluster analysis...
December 2013
Nature of gene action and genetic parameters for yield and its components in chickpea
To determine the gene action and genetic parameters of agro-morphological traits in chickpea, five genotypes of chickpea as a half diallel crossed with each other in 2008, five parents and 10 progenies were planted as randomized complete block design with three replications and some traits including days to flowering and maturity were recorded on row basis when 50% plants flowered or matured. Plant height, biomass,...
December 2013
A bibliometric analysis of global research on genome sequencing from 1991 to 2010
This study was carried out to evaluate the global scientific production of genome sequencing research to assess the characteristics of the research performances and the research tendencies. Data were obtained from Science Citation Index Expanded database during 1991-2010. Conventional methods including document types, journals, categories, countries and institutions were used to analyze publication output to reveal the...
December 2013
The isolation and characterization of twelve novel microsatellite loci from Haliotis ovina
Twelve (12) microsatellite loci were developed from Haliotis ovina by magnetic bead hybridization method. Genetic variability was assessed using 30 individuals from three wild populations. The number of alleles per locus was from 2 to 5 and polymorphism information content was from 0.1228 to 0.6542. The observed and expected heterozygosities ranged from 0.0000 to 0.7778 and 0.1288 to 0.6310, respectively. These loci...
December 2013
Does 2,4-dichlorophenoxyacetic acid induce flowering in sweet potato?
Most sweet potato cultivars grown in Zimbabwe are poor in agronomic and quality traits and require improvement through breeding. However, most cultivars rarely flower yet the flowers are crucial in genetic improvements. The aim of this study was to determine the effects of different levels of 2, 4-dichlorophenoxyacetic acid (2,4-D) on sweet potato flower induction. A 3*4 factorial experiment in a randomized complete...
December 2013
Plant regeneration through indirect organogenesis of chestnut (Castanea sativa Mill.)
To establish an effective protocol for plant regeneration through indirect organogenesis, effects of explants type, culture media and plant growth regulators on callus induction and shoot regeneration of chestnut (Castanea sativa Mill.) were investigated. Three different explants (root, nodal and internodal segment), two different media [Murashige and Skoog medium (MS) and Gamborg's B5 (B5)] and different plant...
December 2013
Protocol optimization for deoxyribonucleic acid (DNA) extraction from dried, fresh leaves, and seeds of groundnut (Arachis hypogaea L.)
Consistent isolation of best quality deoxyribonucleic acid (DNA) from peanut (Arachis hypogaea L.) is particularly problematic due to the presence of phenolic compounds and polysaccharides. Inconsistencies in extraction results can be attributed to the age and growth stages of the plant material analyzed. Mature leaves have higher quantities of polyphenols, tannins and polysaccharides that can contaminate DNA during...
December 2013
Effects of irrigation regimes and polymer on dry matter yield and several physiological traits of forage sorghum var ‘Speedfeed’
Drought is the most important limiting factor for crop production; it is becoming an increasingly severe problem in many regions of the world. Sorghum is among the most important forages used in arid and semi-arid regions of southeastern Iran, but its growth and yield is often constrained by water deficit and poor productivity of sandy soil. Irrigation water is becoming scarcer and more costly. The addition of...
December 2013
Ground water quality evaluation in Beed city, Maharashtra, India
The development activities cause depletion and degradation of ground water. A survey was undertaken to assess the quality of ground water in Beed district of Maharashtra taking both physico-chemical and bacteriological parameters into consideration. The present investigation is aimed to calculate Water Quality Index (WQI) of ground water and to assess the impact of pollutants due to agriculture and human activities on...
December 2013
In vitro antibacterial activity of alkaloid extracts from green, red and brown macroalgae from western coast of Libya
Marine organisms and microorganisms are known to be a rich source of alkaloids with unique chemical feature and interesting biological activities. The current study presents the antibacterial effect of the alkaloid extracts of some green, red and brown algae were collected from western coast of Libya, against, Escherichia coli, Salmonella typhi, Klebsiella spp., and Pseudomonas aeruginosa, Staphylococcus aureus,...
December 2013
Effect of accessions on the chemical quality of fresh pumpkin
Twenty (20) fresh pumpkin fruits were assessed for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, pH, ascorbic acid and sugar-to-acid ratio. Statistically significant (P < 0.01) differences were found for dry matter, total soluble solid, total sugar, reducing sugar, titratable acidity, ascorbic acid, pH and sugar to acid ratio for the 20 fresh pumpkin fruit accessions. Accession...
December 2013
Cactus (Opuntia ficus indica f. inermis) fruit juice protects against ethanol-induced hematological and biochemical damages in rats
A putative beneficial effect of Opuntia ficus indica f. inermis prickly pear juice (OFIj) was tested on ethanol-induced hematological and biochemical damages in rats. Our results show that chronic ethanol treatment (300 mg/100 g body weight for 90 days) of Wistar rats (group 2) significantly reduced red blood cells (RBC) and platelet (Plt) counts, hemoglobin (Hb) content, mean corpuscular hemoglobin (MCH) and mean...
December 2013
Could grape seed extract modulate nephritic damage induced by methomex in male rats?
Methomex (Metho) is classified as a carbamate insecticide. The present study was designed to examine the influence of grape seed oil (GSO) on the histopathological changes in methomex-induced kidney damage in male rats. Rats were divided into 6 groups, the first of which was considered as the control. The 2nd group was treated with 4 ml/kg GSO. Rats from 3rd and 4th groups were treated with Metho at dose level of 2.4...
December 2013
Preventive effect of zinc on nickel-induced oxidative liver injury in rats
This study pertains to the potential ability of zinc, used as nutritional supplements, to alternate oxidative stress induced by nickel. Male rats were randomly divided into four groups of eight each. Group I served as the controls; group II received in their drinking water ZnSO4 (227 mg/l); group III received NiSO4 (2 mg/100b.w/day intraperitoneally); group IV was treated with ZnSO4 and NiSO4. The exposure of rats...
December 2013
Isolation, characterization and antimicrobial activity of Streptomyces strains from hot spring areas in the northern part of Jordan
A total of 30 Streptomyces isolates (28 from soil and 2 from water) were isolated and purified from hot-springs areas in the northern part of Jordan. Four strains were thermopile. They grew at 45 and 55°C but not at 28°C. Strains were described morphologically on four different media: on glycerol yeast extract, oatmeal, yeast malt-extract and starch casein agar. White and grey color series were the most frequent...
December 2013
Effects of dietary conjugated linoleic acid, fish oil and soybean oil on body-fat deposition and serum lipid fractions in broiler chickens
An experiment was conducted on broiler chickens to study the effects of dietary fats rich in conjugated linoleic acid (CLA), fish oil (n-3 rich oil), soybean oil (n-6 rich oil), polyunsaturated fatty acids (PUFAs) alone or in dual mixtures, as well as palm oil as a more saturated fat on tissue fat deposition and serum lipid concentrations of broiler chickens. The fat included in the experiment's diets is a dose of...
December 2013
Helminth parasites of Synodontis nigrita at lower Niger (IDAH), Nigeria
The isolation and identification of helminth parasites of Synodontis nigrita and length–weight relationship of the fish in the lower Niger (Idah), Kogi State, Nigeria were carried out in order to describe the pattern of occurrence of the helminth and to establish the well-being of the host fish. A total of 102 randomly sampled fish were studied and three genera of helminths were recovered; 39.1, 48.7 and 12.2%...
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