African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 19 Apr 2012; 11(32)

April 2012

WRKY transcription factor superfamily: Structure, origin and functions

  WRKY transcription factors are transcriptional regulatory factors in which N-terminal ends contain the WRKYGQR amino acid sequence and a zinc-finger motif. WRKYtranscription factors can regulate the expression of target genes that contain the W-box elements (C/T)TGAC(C/T) in the promoter regions by specifically binding to the (C/T)TGAC(C/T) sequence. WRKY transcription factors regulate the expression of...

Author(s):   Zhao Mingyu,, Zhang Zhengbin, Chen Shouyi, Zhang Jinsong and Shao Hongbo,  

April 2012

Molecular cloning, sequence analysis and structure prediction of the related to b0,+ amino acid transporter (rBAT) in Cyprinus carpio L.

  In this study, the full-length cDNA of basic amino acid transporter gene rBAT was cloned from intestinal cells of Cyprinus carpio L. using reverse transcription polymerase chain reaction (RT-PCR) and rapid-amplification of cDNA ends (RACE) methods. The amplified product was 2370 bp, including a 42 bp 5'-untranslated region, a 288 bp 3'-untranslated region,...

Author(s):   Guoxing Nie, Bei Wang, Junli Wang, Hong Ming, Junlin Zheng, Xuejun Liand Xianghui Kong    

April 2012

Characterization of resistant tomato mutants to bacterial canker disease

  A small scale ethylmethanesulfonate (EMS) mutation was used to obtain resistant mutant plants to bacterial canker disease caused by Clavibacter michiganensissubsp. michiganensis isolate 2 (Cmm2). Susceptible EBR3 tomato line (200) seeds were mutagenised with the chemical EMS. Of the constructed M2 population, 450 seedlings were inoculated with Cmm2. Among the tested M2 plants, 15 M2 plants, not...

Author(s):     Özer ÇALIÅž, Yusuf BAYAN and Demet ÇELÄ°K  

April 2012

Purification and characterization of an extracellular xylanase produced by the endophytic fungus, Aspergillus terreus, grown in submerged fermentation

  Aspergillus terreus produced high levels of a thermotolerant extracellular xylanase and showed low cellulase activity when cultured at 30°C for 48 h, in liquid medium supplemented with wheat bran as carbon source. Xylanase was purified 45-fold to homogeneity with a recovery yield of 67% by carboxymethyl (CM)-cellulose chromatography. The enzyme, a glycoprotein with 33% of carbohydrate content,...

Author(s):   M. Sorgatto, N. C. A. Guimarães, F. F. Zanoelo, M. R. Marques, S. C. Peixoto-Nogueira and G. G. Giannesi  

April 2012

Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea

  Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth with a wide variety of mechanisms. The use of PGPR is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. Here, we have isolated, enumerated and characterized the PGPR from the rhizosphere soil of pigeon pea for the...

Author(s):   M. Usha Rani, Arundhathi and Gopal Reddy    

April 2012

The effect of fungal competition on colonization of soybeans by moulds of the genus Aspergillus, Trichothecium and Fusarium and the ochratoxin A accumulation as related to temperature and moisture content

  The relationship between mould biomass and accumulation of ochratoxin A (OTA) on solid substrate (slightly cracked soybeans) at temperatures from 15 to 35°C and a water content in the substrate of 20 to 38% was been investigated. The experiments were carried out with the mould Aspergillus ochraceus NRRL 3174 in pure culture and in mixed culture, respectively; the latter with the...

Author(s):   Lejla Duraković, Frane Delaš, Alma Tudić and Tihana Jergović-Gaši    

April 2012

A comparative and evaluative study of potential biogas production from crops of teff (Eragrostis tef (Zucc) Trotter) in Ethiopia

  The research on suitable alternative clean energy carriers to substitute for the use of fossil fuels is rapidly attracting attention. Biogas is an energy carrier that is considered as a possible alternative in both the developed and the developing world. However, finding suitable energy crops to extract biogas without affecting food security is still a debatable issue. In this context, the present study was...

Author(s):   Andualem Sitotaw Nigatu, Anna Karlsson and Nicodemus Mandere Mandere  

April 2012

Optimization of bio-ethanol production from cassava effluent using Saccharomyces cerevisiae

  The ethanol producing capability of Saccharomyces cerevisiae from cassava waste water was enhanced. Enhancement was obtained by varying pH, culture age and nutrient supplementation during fermentation until optimum conditions were attained. Optimum fermentation duration for ethanol production in acid and enzyme hydrolysates was 24 h. The start-up fermentation experiment process with S....

Author(s):   Akponah, E. and Akpomie, O. O.  

April 2012

Long-term salt stress responsive growth, carbohydrate metabolism, proline and anti-stress enzymes in Nicotiana tabaccum

  We investigated the long-term responses of tobacco tissues to salt stress, with aparticular interest for growth parameters, proline (Pro) accumulation, and carbohydrate metabolism. Exposure of 17-day-old tobacco plants to 0.2 M NaCl was followed by a higher decrease in dry matter in roots than shoots with a decrease of both Chlorophyll a and b. Analysis of potassium, sodium and chloral...

Author(s):   Chaffei Haouari Chiraz, Hajjaji Nasraoui Afef, Haouari Ali, Carrayol Elisa , Ghorbel Mohamad Habib  and Gouia Houda  

April 2012

Long-term salt stress responsive growth, carbohydrate metabolism, proline and anti-stress enzymes in Nicotiana tabaccum

  We investigated the long-term responses of tobacco tissues to salt stress, with aparticular interest for growth parameters, proline (Pro) accumulation, and carbohydrate metabolism. Exposure of 17-day-old tobacco plants to 0.2 M NaCl was followed by a higher decrease in dry matter in roots than shoots with a decrease of both Chlorophyll a and b. Analysis of potassium, sodium and chloral...

Author(s):   Chaffei Haouari Chiraz, Hajjaji Nasraoui Afef, Haouari Ali, Carrayol Elisa , Ghorbel Mohamad Habib  and Gouia Houda  

April 2012

Characterization of rigor mortis of longissimus dorsi and triceps brachii muscles of male cattle carcasses

  In this work, six bovine carcasses butchered in a slaughterhouse in Rio de Janeiro State, Brazil (SIE 504) were studied and temperature, pH, sarcomere length in different periods after slaughter (1, 5, 8, 12, 15 and 24 h) of the longissimus dorsi (LD) and triceps brachii (TB) muscles as well as the shear force (meat tenderness) and colour were evaluated, aiming at...

Author(s):   Rafael S. Costa, Fábio C. Henry, Karla S. Ferreira, Felipe R. A. F. do Valleand Célia R. Quirino  

April 2012

Synergistic therapy of enalapril and Cordyceps sinensis in the improvement of renal function in chronic allograft nephropathy patient

  Chronic allograft nephropathy (CAN) still remains an important factor that affects the long-term survival of renal recipients. The aim of the study was to investigate synergistic effect of enalapril (an angiotensin converting enzyme inhibitor, ACEI) andCordyceps sinensis (Bailing capsule, fermented agent of C. sinensis) on CAN and pursue an effective therapy to control CAN progression. A...

Author(s):   Zhi-hong ZHANG, Guo-dong LIU, Xiang-wei WANG, Yuan-ning ZHANG and Ying-lu GUO  

April 2012

Flowering in the wild olive (Olea europaea L.) tree (oleaster): Phenology, flower abnormalities and fruit set traits for breeding the olive

    Although, the olive trees produce hermaphrodite flowers, abnormal flowers (flowers with absence or reduced stamens and flowers with absence of pistil) are frequently observed and may reduce fruit set. This study investigates the phenology evolution and the male and female abortion of the oleaster tree (or the wild olive tree) flowers (Olea europaea L. subsp....

Author(s):   Hédia Hannachi and Sizaiem Marzouk  

April 2012

Robust model of fresh jujube soluble solids content with near-infrared (NIR) spectroscopy

  A robust partial least square (PLS) calibration model with high accuracy and stability was established for the measurement of soluble solids content (SSC) of fresh jujube using near-infrared (NIR) spectroscopy technique. Fresh jujube samples were collected in different areas of Taigu and Taiyuan cities, central China in 2008 and 2009. A partial least squares (PLS) calibration model was established based on...

Author(s):     Shujuan Zhang, Yanru Zhao, Wei Guo, Qiaoling Hao, Haihong Zhang, Huamin Zhao and Lihua Yin  

April 2012

Comparative analysis of essential oil contents of Juniperus excelsa (M. Beib.) found in Balochistan, Pakistan

  Cones/berries of Juniperus excelsa from three provenances in Balochistan, Pakistan were collected and essential oil was extracted by solvent method. Oil contents were analyzed on gas chromatography mass spectrometry (GCMS). Identification and quantification was made by using Wiley and NIST spectral library and HP Chemstation software. Quantitatively the mean cone oil yield was 5.8, 6.5 and 4.5% for...

Author(s):   Muhammad Hussain Khajjak, Agha Mohammad Raza, Mohammad Naeem Shawani, Fayyaz Ahmed, Ghazala Shaheen and Mohammaad Saeed  

April 2012

Differentiation of pork from beef, chicken, mutton and chevon according to their primary amino acids content for halal authentication

  The detection of pork in various food products has been an important subject of study in many countries. The current study was aimed to differentiate pork from selected meats of beef, mutton, chevon and chicken based on their primary amino acid contents using reverse phase-high performance liquid chromatography (RP-HPLC)with derivatization by o-phthalaldehyde (OPA) and ultraviolet...

Author(s):   Ramin Jorfi, Shuhaimi Mustafa, Yaakob B Che Man, Dzulkifly B Mat Hashim, Awis Q Sazili, Abdoreza Soleimani Farjam, Leila Nateghi and Pedram Kashiani  

April 2012

In vitro regeneration from internodal explants of bitter melon (Momordica charantia L.) via indirect organogenesis

  Organogenic callus induction and high frequency shoot regeneration were achieved from internodal explants of bitter melon. About 97.5% of internodal explants derived from 30 day old in vivo grown plants produced green, compact nodular organogenic callus in Murashige and Skoog (MS) plus Gamborg et al. (1968) (B5) medium containing 5.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 μM...

Author(s): Muthu Thiruvengadam, Nagella Praveen and Ill-Min Chung