African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 19 June 2012; 11(49)

June 2012

Potential novel bZIP-like gene for resistance to Erysiphe necator identified in Chinese wild Vitis pseudoreticulata

In this study, a novel bZIP-like gene was isolated from Chinese wild Vitis pseudoreticulata W. T. acc. Baihe-35-1. The full-length complementary deoxyribonucleic acid (cDNA) sequence of the gene was 1583 bp including 159 bp 5’ untranslated region (UTR), 365 bp 3’ UTR and a 1083 bp ORF which encodes a polypeptide of 360 amino acids with a molecular weight of 38.662 kDa. The deduced amino...

Author(s): Hongmin Hou,#, Huie Li#, Hao Wang, and Xiping Wang,

June 2012

Expression of VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain under control of the ats1A promoter in tobacco

The VP60 gene from rabbit haemorrhagic disease virus (RHDV) YL strain in Northeast of China, under control of the ats1A promoter from Rubisco small subunit genes ofArabidopsis thaliana, was introduced into the transfer deoxyribonucleic acid (T-DNA) region of plant transfer vector pCAMBIA1300 and transferred to tobacco (Nicotiana tabacum cv. Petit Havanna SR1) with Agrobacterium...

Author(s): Zhang Da-Peng, Lü Ning, Wan Jun-Hua and Guo Ai-Guang

June 2012

Is salinity tolerance of rice lines concerned to endogenous ABA content or to the cellular ability for ABA synthesis under stress?

In this work we tested its putative relationship of Abscisic acid with the degree of tolerance to this abiotic stress. For this purpose, we have examined the responses of sensitive (IR29) and tolerant (IR651) varieties of indica rice (Oryza sativa L.) to a range of salinity (0 (control) and 90 mM NaCl. Shoot and root dry weight reduced and leaf Na concentration increased in response to salinity for both cultivars...

Author(s): Saeed Saeedipour

June 2012

Seed yield and some yield components of sesame as affected by irrigation interval and different levels of N fertilization and superabsorbent

In order to study the effect of different irrigation, N and superabsorbent levels on yield and yield components of sesame, a field experiment was conducted in Khosf Region, Birjand, Iran in 2009 as a split-split plot design based on a randomized complete block design. The treatments included irrigation interval at three levels of six, 12 and 18 days as main plot, N fertilization level at three levels of 0, 100 and 200...

Author(s): Zeinolabedin Jooyban and Seyyed Golamreza Moosavi

June 2012

The effect of polythene colour container and three spawn rates on production of Pleurotus ostreatus mushroom

To study the effect of polythene colour container and spawn rates on mushroom (Pleurotus ostreatus), experiment was carried out to investigate the cultivation on barely straws substrate. This cultivation system can improve the economic status of the farmer. Field experiment was conducted at College of Agriculture, University of Zabol during 2011 growing season. Experiment was carried out as factorial...

Author(s): Mehdi Dahmardeh

June 2012

Fructan metabolism in tall fescue calli under different environmental condition

Several grasses usually accumulate high levels of fructans at low temperature or in the presence of high sugar supply. Fructans enhance plant resistance in adverse environmental conditions thanks to their vacuolar localization which allow osmotic adjustments. Tall fescue calli do not show mature vacuoles due to its anatomic characteristics, and these in vitro cultures can provide more knowledge about the...

Author(s): Cláudia Roberta Damiani, Laura Pistelli and Lorenzo Guglielminetti

June 2012

Analysis of bacterial and fungal community structure in replant strawberry rhizosphere soil with denaturing gradient gel electrophoresis

High quality DNA is the basis of analyzing bacterial and fungal community structure in replant strawberry rhizosphere soil with the method of denaturing gradient gel electrophoresis (DGGE). DNA of soil microorganisms was extracted from the rhizosphere soil of strawberries planted in different replanted years (0, two, six and seven), respectively, and crude DNA was purified after extraction. Three methods were...

Author(s): Anying LI, Yu WEI, Zhenjun SUN, Tengfei FAN and Lusheng ZHANG

June 2012

Agro-industrial potential of sweet sorghum accessions grown under semi-arid conditions

The current world-wide interest in alternative and sustainable forms of energy is providing an opportunity for carving new pathways of development in many countries. Plants are particularly attractive vehicles in this regard because of their innate capacity to self-renew and to produce chemical forms of energy every time. Carbohydrates and oils as primary products from plants are attractive raw materials for the...

Author(s): Peloewetse E.

June 2012

Role of adiponectin/phosphatidylinositol 3-kinase/protein kinase B signaling pathway on limb ischemic preconditioning on myocardial protection

The adiponectin/phosphatidylinositol 3-kinase/protein kinase B (ADP/PI3k/Akt) signal transduction pathway has an important role in promoting cell survival. This study was designed to determine if the ADP/PI3K/Akt signaling pathway has a role in the mechanism of ischemia–reperfusion injury in vivo. Sprague–Dawley rats were divided into five groups of six: Group A was the sham group, group B was the myocardial...

Author(s): Jian-Zhi GAO, Feng-Hua LV, Qing-Lei TENG and Jin-Ying ZHANG

June 2012

Co-expression of apoptin (VP3) and antibacterial peptide cecropin B mutant (ABPS1) genes induce higher rate of apoptosis in HepG2 and A375 cell lines

The antibacterial peptide cecropin B mutant (ABPS1) gene has a broad range of antibacterial and antiproliferative properties. Apoptin (VP3), a chicken anaemia virus-encoded protein is known to induce apoptosis in human transformed cells. To explore drug combination in human tumor cells, apoptin and ABPS1 eukaryotic expression vector pIRES2-EGFP-apoptin and pIRES2-EGFP-ABPS1 were constructed and their...

Author(s): Basse Mame Birame, Wang Jigui, Yu Fuxian, Wang Shuang, Yuan Dao Li, Sun Jiazeng, Li Zhili, Yi Bao and Liu Weiquan

June 2012

Procalcitonin and white blood cell count (WBC), erythrocyte sedimentation rate (ESR) and serum C-reactive protein (CRP) alterations in children with systemic inflammatory response syndrome before and after treatment

Systemic inflammatory response syndrome (SIRS) due to infection is an important cause of morbidity and mortality in children. The present prospective observational study aimed to determine the correlation between procalcitonin (PCT) and white blood cell count (WBC), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) in toddlers before and after treatment. Moreover, 50 patients aged 1 to 36...

Author(s): Masumeh Abedini, Ali Delpisheh, Bahram Nikkhu, Ahmad Vahabi and Abdorrahim Afkhamzadeh

June 2012

Long SAGE analysis of genes differentially expressed in the midgut and silk gland between the sexes of the silkworm Bombyx mori

There are great differences in silk production efficiency and quality between the male and female domestic silkworm (Bombyx mori). Many genes act together but are differentially expressed between the sexes during silk biosynthesis. Two long serial analyses of gene expression (SAGE) libraries were constructed from the midguts and silk glands of both males and females of a sex-limited strain using 5th instar larvae,...

Author(s): Liping Gan, Ying Wang, Jian Xi, Yanshan Niu, Hongyou Qin, Yanghu Sima and Shiqing Xu

June 2012

Synthesis of silver nanoparticles using a probiotic microbe and its antibacterial effect against multidrug resistant bacteria

In this paper, a probiotic microbe mediated biosynthesis of silver nanoparticles (AgNPs) is reported. The synthesis of AgNPs was monitored by ultraviolet (UV)-visible spectroscopy. The particles thereby obtained were characterized by UV spectra, Fourier transform infrared (FTIR), scanning electron microscopy (SEM) with energy dispersive spectroscopy (EDS) and using particle size analysis. The mechanism leading to...

Author(s): Ranganathan Nithya and Ramachandran Ragunathan

June 2012

Antifungal activity of different extracts of Ageratum conyzoides for the management of Fusarium solani

Ageratum conyzoides L. is potential allelopathic weed very useful for its antifungal and antimicrobial activity. Being environmentally safe and friendly, it has the potential to substitute synthetic fungicides. The current study, therefore, was designed to evaluate the in vitro efficacy of aqueous methanolic and n-hexane extracts of A. conyzoidesagainst the pathogenic fungi, Fusarium...

Author(s): Sidra Javed and Uzma Bashir

June 2012

Effects of a novel recombinant somatostatin DNA vaccination on rat fertility and offspring growth

In this study, we investigated the immune effects of a novel somatostatin (SS) DNA vaccine—pVAX-asd-GS/2SS (pGS/2SS), administrated with intramuscular (IM) and subcutaneous (SC) two delivery routes on female rat fertility and offspring growth. Results show that this pGS/2SS DNA vaccine could induce effective anti-SS immune response in rats (IM group and SC group). The antibody peak of female rats in IM group...

Author(s): Jian Zhang, , Aixin Liang, Liya Bai, Liguo Yang and Jiangang Gao

June 2012

Genetic diversity and population structure of Caragana microphylla Lam. based on analysis of inter-simple sequence repeat markers

Caragana microphylla Lam. is a long-lived shrub species in the semi-arid, arid and desert regions. To determine the genetic diversity and population structure of C. microphylla Lam., 17 wild populations from the central and eastern part of Inner Mongolia were analyzed by inter-simple sequence repeat. 18 primers produced 296 bands across a total of 510 individuals. A high percentage of polymorphic loci was...

Author(s): B. Xu, H. W. Gao, Z. Wang and J. W. Lu