June 2013
Detection of viruses in seeds of some vegetables by reverse transcriptase polymerase chain reaction (RT-PCR)
The present study was carried out to detect the presence of Alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV), lettuce mosaic potyvirus (LMV), cucumber green mottle mosaic virus (CGMMV), tomato bushy stunt tombusvirus (TBSV), tobacco mosaic tobamovirus (TMV), tomato black ring nepovirus (TBRV) and tomato mosaic tobamovirus (ToMV) in seeds of pepper, tomato, cucumber and lettuce which are...
June 2013
Alteration of rice growth and development via antisense expression of OsGA20ox2 gene
GA 20-oxidase is a key enzyme involved in gibberellin (GA) biosynthesis. To investigate the roles of OsGA20ox2 in regulating plant growth and development, we used reverse genomic approach to generate dwarf transgenic rice plants with antisense technology. OsGA20ox2 nucleic acid sequence includes three exons and two introns. We constructed three antisense vectors named pA1CK, pA2CK, pACK,...
June 2013
Amplification of the active site of BnLIP3 gene of Brassica napus L. during germination
Lipases are useful enzymes that are responsible for the hydrolysis of triacylglycerides and play an important role in plant growth. In this study, we report a rapid molecular method to amplify a partial sequence of the lipase class 3 family designated BnLIP3gene of Brassica napus L. in order to follow its expression and analyze its role during seed germination. Therefore, we conceived PCR...
June 2013
Molecular characterization of olive cultivars grown in Iraq using amplified fragment length polymorphism and simple sequence repeat markers
In this study, genetic relationships among olive cultivars grown in Iraq were investigated by means of two DNA molecular marker classes: amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR). Total genomic DNA was isolated from young leaves of the selected olive...
June 2013
Optimisation of phenolic compound biosynthesis in grape (Bogazkere Cv.) callus culture
The objective of this study was to improve metabolite accumulation of grape cells by manipulating culture and environmental conditions. Response surface methodology (RSM) was used to design and conduct the study with the independent variables being: percent depletion of KH2PO4 or NH4NO3 and light intensity. Wounded grape leaves (Bogazkere cv.) were used for the initiation of callus formation....
June 2013
Differences between the Texas phoenix palm phytoplasma and the coconut lethal yellowing phytoplasma revealed by restriction fragement length polymorphism (RFLP) analysis of the NUSA and HFLB genes
A nested polymerase chain reaction (PCR) assay was used to amplify the nusA gene from a DNA sample extracted from a cabbage or sabal (Sabal palmetto) palm showing symptoms of Texas Phoenix decline (TPD) and from three DNA samples from coconut (Cocos nucifera) palms showing symptoms of lethal yellowing (LY). TPD is caused by a 16SrIV-D phytoplasma and LY by a 16SrIV-A strain. From the sabal DNA...
June 2013
Tissue culture as an alternative for commercial corm production in saffron: A heritage crop of Kashmir
The present study aimed at developing a commercially viable protocol for in vitro corm production in saffron. Three-step sterilization process involving fungicides and sterilants ensured 94% clean viable cultures. Plant growth regulator (PGRs) ensuring initial bud sprouting, direct shoot regeneration from the base of the sprouted bud and cormlet production from multiple shoots have been...
June 2013
Total β-carotene content of orange sweetpotato cultivated under optimal conditions and at a rural village
At community level, sweetpotato is often cultivated in marginal soils, with low agricultural inputs and is harvested when needed. Total β-carotene content of orange sweetpotato harvested four, five and six months after planting at optimal cultivation conditions and at a rural village was determined. Compared to those produced under optimal conditions, sweetpotato produced under rural village...
June 2013
Probable functions of calcium oxalate crystals in different tissues of the edible aroids (Xanthosoma and Colocasia spp.) in Nigeria
Representatives of seven major edible aroid accessions were screened for calcium oxalate using standard histochemical methods. All the accessions were noted to contain calcium oxalate in the forms of raphide bundles and intra-amylar crystals. The crystals were widely present in all parts of the plants including spongy mesophyll of the leaves, laticifers in the midrib and petioles, cortex of the roots and in...
June 2013
Influence of Glomus etunicatum and Glomus intraradices fungi inoculums and micronutrients deficiency on root colonization and dry weights of tomato and sorghum in perlite bed culture
The objectives of this study were to determine the effects of micronutrient deficiency on root colonization by arbuscular mycorrhizal fungi (AMF), and to assess the role of AMF on growth of sorghum and tomato plants in perlite bed culture. In a pot culture experiment with sterile perlite, sorghum (Sorghum bicolor L.) and tomato (Lycopersicon esculentum Mill.) plants were inoculated with...
June 2013
Establishment of Eucalyptus grandis W. Hill ex Maiden in vitro using commercial products for seed treatment
Given the current demand for timber from forest species, there is a need to develop new strategies for the mass propagation of eucalyptus. Therefore, this study aimed to determine the effect of different doses of Standak Top® and CoMo Raiz® on the establishment of Eucalyptus grandis W. Hill ex Maiden from seeds in vitro. The experimental...
June 2013
Biochemical responses during the pathogenesis of Sclerotium rolfsii on cowpea
The hypersensitive reaction (HR), one of the most efficient and visible parts of the defense mechanisms in nature against invading pathogens, is associated with a coordinated and integrated set of metabolic alterations which are instrumental in impeding further pathogen ingress or alleviating stress. It includes a variety of novel proteins and secondary metabolites. This study aimed to examine the induction...
June 2013
Estimation of genetic parameters for yield components and seed yield in sunflower using line x tester analysis
Combining ability and heritability of yield and yield components of sunflower (Helianthus annuus L.) breeding lines were studied using line x tester analysis. Eight lines and six testers along with their 48 F1 single cross combinations and two checks were planted in simple lattice design with two replications. The results of analysis of variance revealed significant differences among the genotypes for...
June 2013
Agricultural and Natural Resources Research Center of Mazandran, Sari, Iran.
The aims of the study were to evaluate water quality of Tajan River in Sari in terms of chemical pollution and the impact of pollutant sources near the river by considering the climate, hydrological and hydraulic condition on it. In this study, 10 critical points of river were selected as sampling stations from dam to sea. Sampling was randomly done monthly from Apr 2009 to Mar 2010. Samples were analyzed in...
June 2013
Warm season performance of horizontal subsurface flow constructed wetlands vegetated with rice treating water from an urban stream polluted with sewage
The performance of horizontal subsurface flow constructed wetlands vegetated with rice (Oryza sativa L.) was investigated in Campina Grande (7º 13' 11" S; 35º 52' 31" W; 550 m above mean sea level), Paraíba state, northeast Brazil. The pilot-scale system comprised 24 circular tanks (76.80 cm diameter, 54 cm height) batch fed daily with water from a nearby urban...
June 2013
Mixed cultures of Kimchi lactic acid bacteria show increased cell density and lactate productivity
This study was carried out to determine the characteristics of cell growth, lactate production and amino acid secretion among four kimchi lactic acid bacteria (Leuconostoc mesenteroides JEI, Leuconostoc kimchi 132, Lactobacillus sakei 171, and Weissella koreensis 521) alone and in selected mixtures. In solo culture, L. sakei171 was superior in cell growth, lactate...
June 2013
Seasonal variation in chemical composition, aroma volatiles and antioxidant capacity of pomegranate during fruit development
This study was conducted to investigate compositional changes and antioxidant capacities of pomegranate fruit (‘Bhagwa’ and ‘Ruby’) at five distinct stages of maturity over two growing seasons. Total soluble solids (TSS), pH, titratable acidity (TA), phenolic concentrations, antioxidant capacity and aroma volatile constituents were investigated. Principal component analysis (PCA) and...
June 2013
Extracellular proteolytic activity of Deinococcus geothermalis
Production of extracellular protease by extremophilic bacteria Deinococcus geothermalis cultivated in liquid media containing 0.1% (w/v) of peptone K, 0.1% yeast extract and 0.2% marine salt reached a maximum in 14 h of the cell growth at 45°C and pH 8.0. The enzyme was purified by a two-step procedure using fractionation by a graded ammonium sulphate precipitation technique and gel...
June 2013
Genotoxicity and oxidative stress induced by cadmium and zinc in the planarian, Dugesia dorotocephala
This study sought to determine the DNA damage, and the lipoperoxidative effect, as well as changes in the superoxide dismutase (SOD) and catalase (CAT) activities induced by CdSO4 (Cd), and ZnSO4 (Zn), in addition to two different mixtures of the metals. Planarian Dugesia dorotocephala collected in the Ignacio Ramirez reservoir, México, adapted to laboratory conditions, and exposed...
June 2013
Bioevaluation of garlic on growth, haematological and serum characteristics of growing pigs
A total of 18 grower pigs aged 70 days were used in an eight weeks feeding trial to evaluate the biological importance of feeding varying dietary levels of sun dried garlic to pigs. The 18 pigs were randomly assigned to three dietary treatments (T1, T2 and T3) with 0, 100 and 200 g of garlic powder incorporated per 100 kg of feed, respectively. Each treatment was replicated twice with three pigs per...
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