African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 20 November 2012; 11(93)

November 2012

Advances in Ginkgo biloba research: Genomics and metabolomics perspectives

  The maiden hair tree, Ginkgo biloba is very much resistant to a wide spectrum of biotic and abiotic stress conditions. It hardly seems to be attacked by any herbivore or microbe. In spite of its strong resistant nature to wide stress conditions, only little research has been carried out at genomics and metabolomics level to understand its mechanism of stress resistance. Ginkgolide and bilobalide, the...

Author(s): Tapan Kumar Mohanta

November 2012

T-786C single-nucleotide polymorphism (SNP) of endothelial nitric oxide synthase gene and serum level of vascular endothelial relaxant factor (VERF) in non-diabetic patients with coronary artery disease

Various mutations on endothelial nitric oxide synthase (eNOs) gene cause reduced production of NO, a vascular endothelial relaxant factor (VERF), and may accelerate the process of atherosclerosis. The study was designed to investigate the frequency of T-786C polymorphism of the gene in patients suffering from coronary artery disease (CAD) in North West of Iran. One hundred and twenty (120) subjects including...

Author(s): A. R. Yaghoubi and F. Khaki-Khatibi

November 2012

Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

  The structure and organization of the genome of Agave is still unknown. To provide a genomic tool for searching sequences of the genus, we built and characterized a binary (BIBAC2) genomic library of Agave tequilana Weber var. azul. Clones of the library had an average insert size of 170 Kb. The frequency of inserts with internal Not I sites was 30% and only 5% of the library showed...

Author(s): Tamayo-Ordoñez, M., Rodríguez-Zapata, L. C. and Sánchez-Teyer, L. F.

November 2012

Assessment of genetic diversity of nine hybrid combinations of canola using SDS-PAGE, RAPD and ISSR markers

  Genetic variations of nine hybrid combinations of canola were determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. The protein profile did not show any polymorphism. The RAPD and ISSR primers recorded a high degree of polymorphism among generations of the three canola hybrids....

Author(s): Shawkat Mahmoud Ahmed

November 2012

Phenotyping and molecular characterization of Lysinibacillus sp. P-011 (GU288531) and their role in the development of Drosophila melanogaster

  The bacterial strain Lysinibacillus sp. (P-011) was isolated from the midgut of theDrosophila melanogaster larvae. The bacteria were gram positive, spore forming, rod shaped ranging from 1.86 to 2.5 μm in length and 0.50 to 0.67 μm in diameter, positive for catalase, indole, oxidase, nitrate reduction, starch and gelatin hydrolysis, sensitive to tetracycline,...

Author(s): Priyanka Maji, Chandrasekhar Chakrabarti and Soumendranath Chatterjee

November 2012

Evaluation of drought tolerance indices for the selection of Iranian barley (Hordeum vulgare) cultivars

  Drought is an important factor limiting crop production in arid and semi-arid conditions. Drought indices which provide a measure of drought based on yield loss under drought condition in comparison to normal condition was used for screening drought-tolerant genotypes. This study was conducted to determine drought tolerant genotypes with high yield in stress and non-stress conditions. Ten barley genotypes...

Author(s): Mahdi Zare

November 2012

In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis

  This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA3) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed germination. The highest direct organogenic...

Author(s):   Bello-Bello Jericó, Iglesias-Andreu Lourdes, Sánchez-Velásquez Lázaro, Casas-Martínez José and Santana-Buzzy Nancy

November 2012

Phenology, productivity, and chemical characterization of Jatropha curcas L. as tool for selecting non-toxic elite germplasm

  A phenological, physical and chemical descriptive study was carried out on six germplasms of Jatropha curcas L. collected from different areas of Mexico. From these six germplasms, elite germplasms that are better adaptable, with high seed, oil and protein yield, and low levels of phorbol esters were identified. The thermal constant (2570 ± 9) and seed weight (0.695 ± 0.065 g) were...

Author(s): Maria P. Sosa-Segura, Miguel A. Angulo-Escalante, Jose B. Valdez-Torres, Jose B. Heredia, Tomas Osuna-Enciso, Raul Allende-Molar and B. Dave Oomah

November 2012

Effect of eucalyptus (Eucalyptus camaldulensis) and maize (Zea mays) litter on growth, development, mycorrhizal colonization and roots nodulation of Arachis hypogaea

  In Senegal, farmers often cultivate groundnut in association with eucalyptus plantations to increase their incomes. However eucalyptus plantations produce large amounts of litter, which impact on groundnut has not been clearly elucidated yet. In order to investigate litter accumulation effect on growth, development, and groundnutroot infection by arbuscular...

Author(s): Abdoulaye SOUMARE, Saïdou Nourou SALL, Georges Anicet MANGA, Mohamed HAFIDI, Ibrahima NDOYE, and Robin DUPONNOIS,

November 2012

Micropropagation of marula, Sclerocarya birrea subsp. caffra (Anarcadiaceae) by axillary bud proliferation and random amplified polymorphic DNA (RAPD) analysis of plantlets

The availability of a rapid vegetative amplification procedure of mass-selected superior trees greatly accelerates the development of a new tree species as a crop. This study outlined the protocol for in vitro propagation of marula nodal explants from marula seedlings. Surface sterilized explants were cultured on Murashige and Skoog media (MS) supplemented with 26 combinations of N6-benzyladenine (BA) and...

Author(s): Margaret H. N. Mollel and Elisabeth M. A. Goyvaerts

November 2012

Combined protective effect of vitamins C and E on cadmium induced oxidative liver injury in rats

  Our study pertains to the potential ability of vitamin C and/or vitamin E, used as nutritional supplements, to alleviate oxidative stress induced by cadmium. Male rats were randomly divided into five groups of eight each. Group I served as the controls; group II received in their drinking water CdCl2 (200 mg/L); group III received bothCdCl2 and vitamin C (1.5 g/L of water);...

Author(s): Naima Layachi and Zine Kechrid

November 2012

Fundamental conditions required in extracting an alpha-amylase from Cadaba farinosa Forsk branches

  Crude extracts of Cadaba farinosa, Forsk plant are used for their starch-reducing power in most tropic countries to liquefy/sweeten starch gruels. The extraction process is a time consuming osmotic rehydrating process characterized by fluctuating extract yield and consequently, an unstable inherent starch-reducing power. In this study, dried tender branches were ground (Ø≤1 mm) and...

Author(s): Aseaku J. Nkengbeza and Emmanuel J. Nso

November 2012

Reproductive performance by dairy cows fed supplemental chromium-methionine (Cr-Met) in transition period

  This study was conducted to study the effect of supplemental chromium on reproductive performance of dairy cows. Thus, forty multiparous Holstein cows (parity 3) were allocated to two treatments and 20 replicates in a completely randomized design. In this study, treatments consisted of: control group, which received no chromium supplementation and treatment group, which received 6 g/day chromium from chromium...

Author(s): M. Khalili, A.D. Foroozandeh, M. Toghyani and Gh. Ghalamkari

November 2012

Development of an indirect enzyme-linked immunosorbent assay (ELISA) assay based on a recombinant truncated VP2 (tVP2) protein for the detection of canine parvovirus antibodies

  By removing the N-terminal hydrophobic sequence, truncated VP2 (tVP2) genes were cloned into the pET-32a (+) plasmid and subsequently expressed as His fusion proteins. The purified recombinant tVP2 proteins were specific to canine parvovirus (CPV), and one of them was used in an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of CPV antibodies. The minimum detection limit of this method...

Author(s): Lijun Shi, Jing Wang, Peng Wang, Gang Li, Miaomiao Gong, Weifeng Yuanand Hongfei Zhu