August 2013
Optimization of DNA extraction and amplified fragment length polymorphism (AFLP) analysis of pomegranate (Punica granatum L.)
Pomegranate (Punica granatum L.) is a plant rich in polysaccharides, polyphenols and secondary metabolites, which makes it difficult to obtain high quality DNA. The present study reports a quick, simple and inexpensive method to isolate genomic DNA suitable for amplified fragment length polymorphism (AFLP) analysis and other PCR-based applications. This method is a modification...
August 2013
Selected hematological and immunological parameters in pigs transferred from the rearing unit to the finishing house
The aim of the study was to determine how selected hematological and immunological parameters are affected when growing pigs are transferred from the rearing unit to the finishing house. Blood was collected from 64 healthy growing pigs one week before and one week after they were transferred to the finishing house. The following tests were performed: complete blood count with...
August 2013
Variability in seed traits, oil content and genetic diversity in local and exotic accessions of Jatropha curcas L. in Senegal
Variability in seed traits, oil content and genetic diversity of Jatropha curcas L. according to rainfall gradient in Senegal are hereby reported and discussed. Seed oil variability ranged from 58.61% in Sudanian zone to 46.94% in Sahelian zone. Seed oil content and seed thickness were correlated to rainfall with a correlation coefficient of 0.62 and 0.48 respectively. However,...
August 2013
Ameliorative effects of salt resistance on physiological parameters in the halophyte Salicornia bigelovii torr. with plant growth-promoting rhizobacteria
Salicornia bigelovii is a promising resource to cultivate under extreme climatic conditions of arid-desert regions. However, the production of Salicornia depends on the appropriate supplementation of nitrogen rich synthetic fertilizers. Application of specific halotolerant nitrogen-fixing bacteria associated with S. bigelovii could be an important practice for crop production in...
August 2013
Influence of biofortification with provitamin A on protein, selected micronutrient composition and grain quality of maize
Provitamin A-biofortified maize is currently being evaluated for use in the alleviation of vitamin A deficiency. Apart from the differences in provitamin A content, the nutritional composition of provitamin A-biofortified maize compared to white maize is hardly known. This study aimed to evaluate the protein and selected micronutrient composition of biofortified maize varieties...
August 2013
Expression analysis of OsbZIP transcription factors in resistance response by the rice blast resistance gene Pi36-mediated
Plant basic leucine zipper (bZIP) proteins play an essential role in the genes expression and regulation in higher plants. They have been shown to regulate diverse plant specific phenomena, including germination, floral induction and development, seed maturation, photomorphogenesis, biotic and abiotic stresses. Resistance response mediated by the rice blast resistance gene Pi36...
August 2013
Variations in fatty acid composition during maturation of cumin (Cuminum cyminum L.) seeds
Changes in fatty acids were studied during maturation of cumin (Cuminum cyminum L.) seeds cultivated in the North-Eastern region of Tunisia (Menzel Temim). The fruits matured in 49 Days after flowering (DAF). The first results show a rapid oil accumulation started in newly formed fruits (8.2%) and continued until their full maturity (16.9%). During fruit maturation, fatty acid...
August 2013
Evaluation of antimicrobial and antioxidant properties of leaves of Emex spinosa and fruits of Citrillus colocynthis from Saudi Arabia
The crude methanol extract of Citrullus colocynthis fruit and Emex spinosa leaves were examined for antimicrobial and antioxidant potentialities. The phytochemical analysis revealed presence of some bioactive principles, such as alkaloids, flavonoids and anthraquinones for E. spinosa and saponin, flavonoids, terpenoids and alkaloids for C. colocynthis. The antimicrobial...
August 2013
Chemical composition and antioxidant activity of essential oil, various organic extracts of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco
In the present work, we studied the chemical composition of the essential oil of Cistus ladanifer and Cistus libanotis growing in Eastern Morocco. The essential oils were obtained by hydrodistillation and their chemical composition was analysed using gas chromatography- mass spectrometry (GC–MS). Camphene, borneol, cyclohexanol-2, 2, 6 tremethyl, terpineol-4 and...
August 2013
Screening of verotoxin-producing Escherichia coli (VETC) O104-2011 from Egyptian market in 2011
Escherichia coli strains are important causes of diarrheal disease in the world and remain a major public health problem of animals and human. Sixty seven samples from different kind of food, water, soil and composit were screened for the detection of verotoxin-producing E.coli (VTEC) in the Egyptian markets from different location. Result showed that none of the samples gives...
August 2013
Dietary effect of rice milling waste and supplementary enzyme on performance of broiler chicks
A four- week study was conducted to determine the effects of feeding rice milling waste (RMW) and supplementary enzyme (Roxazyme G2®) on the performance of broiler chicks. One hundred and twenty (120) 7-day old broiler chicks of Anak strain were randomly divided into eight groups of 15 birds each. The groups were randomly assigned to 8 isocaloric (2.85 Mcal of ME/kg) and...
August 2013
Physicochemical and antioxidant characteristics of Baccaurea angulata fruit juice extract
The purpose of this study was to explore the physicochemical and antioxidant characteristics of Baccaurea angulata fruit juice extract. Freeze-dried whole fruit (FDWF), freeze-dried berry (FDB), and freeze-dried skin (FDS) of B. angulata were analyzed for total phenolic, total flavonoid, total anthocyanin, and antioxidant activities. FDS recorded the highest moisture and ash...
August 2013
Relationship between soil contents and plasma levels of selenium, chromium and manganese in healthy adult Nigerians
The soil contents of trace elements selenium, chromium and manganese were measured to determine their impact on the plasma levels of 160 healthy adult Nigerians in five different experimental locations in Cross River and Akwa Ibom States, South - South Nigeria. The mean (±SD) soil selenium, chromium and manganese concentrations were 2.86 ± 3.97, 15.93 ± 3.35...
August 2013
An effective lipid-producing fungal sp. strain DGB1 and its use for biodiesel production
Among 30 fungal isolates screened for lipid production using Nile-red staining assay, an isolate designated DGB1 was recorded as the highest lipid producer with lipid content up to 40% (w/w). Based on morphological, biochemical and molecular analysis, DGB1 was identified as fungal sp. strain DGB1. Under the optimized time course, lipid content of DGB1 reached its maximum yield...
August 2013
Purification and properties of N-acetyl-β-D glucosaminidase from bovine testicle
N-Acetyl-β-D-glucosaminidase (NAGase) from bovine testicle was purified by ammonium sulfate fractionation followed by diethylaminoethyl (DEAE)-cellulose (DEAE-32) and Sephacryl S-300 chromatography. The enzyme was purified to homogeneity as analyzed by polyacrylamide gel electrophoresis (PAGE) and isoelectric focusing gel electrophoresis (IFGE). The specific activity of the purified...
August 2013
Monitoring of uncultured Dunaliella sp. in an Egyptian solar saltern field based on RuBisCO-encoding gene cbbL
Culture-independent molecular approach was used to explore and evaluate the diversity of Dunaliella species living at the salt field Malahat El-Max Alexandria, Egypt. Bulk genomic DNA was extracted directly from the collected salt water samples. Specific PCR primers and methodology were designed to amplify the gene cbbL, which encodes the large subunit of the enzyme...
August 2013
Quality of field collected and laboratory reared Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) for screening maize genotypes
The quality of laboratory reared stem borer species for screening of maize varieties is usually questioned by end user cereal breeders. A quality check study was performed in a screen house at KARI-Katumani to evaluate the quality of eight-year old laboratory reared stem borer, Chilo partellus (Swinhoe). The evaluation was aimed at finding out the performance of the laboratory...
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