African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 23 January 2013; 12(4)

January 2013

Ozone effects on forests

  Tropospheric ozone concentration has been rising in the last decades, due to industrial and other human activities. For plants, ozone constitutes one of the most damaging air pollutants. Main effects of ozone on forest species are reviewed: visible symptoms caused by acute exposure at the anatomical, structural and metabolic level, and the long run effects on growth and development derived from chronic...

Author(s):   Gomez-Garay Arancha#, Calderon Guerrero Carlos#, Saiz de Omeñaca José Antonio and Manzanera J. A      

January 2013

Genetic relationships among Vigna mungo (L.) Hepper and its close wild relatives using microsatellite markers

  Genetic relationships of two wild Vigna species, Vigna mungo var. silvestris andVigna hainiana, with accessions of a cultivated species, V. mungo known as urdbean or blackgram was studied with a view of using them as a donor of alien stress resistance genes in future breeding. POPGENE analysis conducted on a total of 375 individuals of 25 accessions using sequence...

Author(s):   Kole, P. R., Saxena, S., Mathew, J. and Bhat, K. V.        

January 2013

Adenosine diphosphate-ribosylation factor 1 controls spindle assembly during first meiotic division in bovine oocytes

  Adenosine diphosphate-ribosylation factor 1 (ARF1) is a member of guanosine triphosphate (GTP)-binding proteins family associated with Golgi complexes. ARF1 regulated asymmetric cell division in female meiosis in mouse. However, little isknown about its function in bovine oocyte meiosis. In the present study, we examined the localization, expression and functions of ARF1 during bovine oocyte...

Author(s):   Yun-An Shi, Yong Jing, Wei Li, Wen-Lin Yang, Xiao-Ling Ma, Miao-Miao Ma and An-Min Lei        

January 2013

Haploid embryos of lettuce (Lactuca sativa) induced by alien pollen or chemical factors

  In order to work out an effective haploidization method in lettuce (Lactuca sativa), we attempted to induce the development of haploid embryos by chemical treatment of pistils or through wide crossing of lettuce with 25 species (mostly of the Asteraceae family). The highest frequency of embryos was achieved after crosses withHelianthus annuus (16%) or H. tuberosum (19%). All the embryos,...

Author(s):   Piosik Łukasz    

January 2013

Characterization and comparison of key genes involved with flowering time regulation from Arabidopsis thaliana, Oryza sativa and Zea mays

  Flowering is an important phase in the angiosperms life cycle, as it signifies the transition from vegetative to reproductive phase. Recently, increasing number of flowering genes are being cloned and analyzed. However, few studies have focused on the comparative analysis of key candidate genes involved in the regulation of flowering time across species. We explore the common characteristics of key flowering...

Author(s):   Bi Ma, Lijuan Wei, Annaliese S. Mason, Zeshan An, Meili Xiao, S. S. Banga, Junjun Zhang, Qinghong Zhou and Donghui Fu      

January 2013

Preliminary characterization of genetic strains in clariid species, Clarias gariepinus and Heterobranchus bidorsalis using microsatellite markers

  Two Clariid species, Clarias gariepinus and Heterobranchus bidorsalis were compared at four loci using C. gariepinus microsatellite markers (Cga01, Cga02,Cga03, and Cga05). The heterozygosities observed were found to be 0.450 ± 0.050 and 0.442 ± 0.127, while 100% polymorphism was observed in both species. Overall, 95% of the samples amplified upon...

Author(s):   O. T. Agbebi, D. E. Ilaboya and A. O. Adebambo        

January 2013

Cultural, morphological, pathogenic and molecular characterization of Alternaria mali associated with Alternaria leaf blotch of apple

    Alternaria blotch (Alternaria mali) causes severe foliar damage to apple trees in Kashmir. Twenty one (21) isolates of A. mali were collected from different locations and characterized for cultural, morphological, pathogenic and molecular variations. A. mali colonies varied in their cultural behaviour ranging from velvety to cottony, mostly appressed, with regular to irregular...

Author(s):   T. A. Sofi, Muzafer A. Beig, Gh. Hassan Dar, Mushtaq Ahmad, Aflaq Hamid, F. A. Ahangar, B. A. Padder and M. D. Shah        

January 2013

Application of chitosan solutions for rice production in Vietnam

  Preparing chitosan solutions from shrimp shells for rice production was investigated. The chitosan produced from shrimp shells using dilute acetic acid proved effective in controlling plants infection by microbial agents leading to higher yields. The field data of our studies showed that the yields of rice significantly increased (~31%) after applying chitosan solution. In general, applying chitosan increased...

Author(s):   Nguyen Van Toan and Tran Thi Hanh        

January 2013

Responses of selected biota after biostimulation of a vegetable oil spill in the Con Joubert Bird Sanctuary wetland: A pilot study

  An investigation on the effect of a vegetable oil spill was conducted on the biological diversity of the  Con Joubert Bird Sanctuary wetland in South Africa before and after biostimulation with different  concentrations of fertilizer during 2008. Biostimulation responses were analyzed 30 days after different  concentrations of fertilizer were applied to the freshwater wetland at...

Author(s):   Mapurunyane C Selala, Paul J Oberholster., Karen AK Surridge,  Arno R de Klerk, and  Anna-Maria Botha

January 2013

Characterization of Pseudomonas aeruginosa PB112 (JN996498) isolated from infected Labeo bata (Hamilton) by 16S rRNA gene sequence analysis and fatty acid methyl ester (FAME) analysis

  In this study, a total number of fifteen moribund Labeo bata were collected from Hooghly, Purba Medinipur, and South 24 Parganas districts of West Bengal, India. The lesions and hemorrhages were recorded at the base of the fins or on the skin due to bacterial infection. Isolation and pure culture of the causative agent were made in the laboratory. The 16S rRNA gene sequence of...

Author(s):   Somerita Panda, P. K. Bandyopadhyay and S. N. Chatterjee         

January 2013

Gum acacia coating with garlic and cinnamon as an alternate, natural preservative for meat and fish

  Meat and fish, the most perishable foods need to be preserved preferably with natural preservatives. In our study, we have proven that spices like garlic and cinnamon that are used as regular ingredients in cooking can act as preservative due to their rich antibacterial and antioxidant profile. The minimum inhibitory concentration (MIC) determined by macrobroth dilution method against five spoilage and...

Author(s):   Madhumita Rakshit and C. Ramalingam        

January 2013

Changes in liver and plasma enzymes of Clarias gariepinus exposed to sublethal concentration of diesel

  This study investigated the effects of graded levels of sub-lethal concentrations of diesel on the biochemistry profile of Clarias gariepinus after exposure for a period of 28 days. 118 (male and female) C. gariepinus were used for the study. They were divided into four groups. Group 1 served as the untreated control (0) while Groups 2, 3 and 4 were treated with graded levels of diesel...

Author(s):   Ezenwaji, Ngozi Evelyn, Yenagoa, Bebe and Nwaigwe Chukwuemeka        

January 2013

The intelligent estimating of spinal column abnormalities by using artificial neural networks and characteristics vector extracted from image processing of reflective markers

  Spinal column abnormities such as kyphosis and lordosis are the most common deformity that normally compare to the standard norms. To classify the subjects into the healthy and abnormal groups based on the angle values of the standard norms, the aim of this study was to use the artificial neural network method as a standard way for realizing the spinal column abnormalities. In this way, 40 male students (26...

Author(s):   Mohammad Yousefi and Saeed Ilbeigi        

January 2013

Isolation, cloning and molecular characterization of a thermotolerant xylanase from Streptomyces sp. THW31

  A xylan-degrading Streptomyces sp. THW31 was isolated from rubbish compost in Thailand. Analysis of a genomic library of nucleotide sequences from Streptomycessp. THW31 revealed that the complete open reading frame (ORF) of xylanase (xlnW31) was 999 bp, and this gene encoded a member of the glycosyl hydrolase family 11. Sequence homology of the predicted amino acid sequence encoded...

Author(s):   Thayat Sriyapai, Peechapack Somyoonsap, Supatra Areekit, Paisarn Khawsak, Arda Pakpitcharoen and Kosum Chansiri    

January 2013

Efficacy of aqueous and ethanolic extracts of leaves of Chromolaena odorata as molluscicide against different developmental stages of Biomphalaria pfeifferi

The molluscicidal activities of aqueous and ethanolic extracts of leaves of Chromolaena odorata were investigated on adult, one week old juveniles and 3 to 4 days old egg-masses of Biomphalaria pfeifferi. Ten adults each were exposed to a serial dilution of 40, 80, 160, 240, 320, 400, and 480 ppm distilled water extracts and 20, 40, 80, 160, 240 and 320 ppm ethanolic extracts of leaves of C. odorata. Twenty juveniles of...

Author(s): Benson Otarigho and Olajumoke A. Morenikeji