African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 24 July 2012; 11(59)

July 2012

Development of a sequence characterized amplified region (SCAR) marker for identification of Thai jasmine rice (Oryza sativa L.) variety ‘KDML105’

The varieties of rice are difficult to be distinguished because of their similar morphological characters, therefore rice variety identification or differentiation is very important. The authentication by using molecular marker or DNA fingerprinting is the most accurate method. In this study, random amplified polymorphic DNA (RAPD) derived sequence characterized amplified region (SCAR) markers were applied to...

Author(s): Juthaporn Saengprajak

July 2012

Mutational analysis of fructose-1,6-bisphosphate aldolase of Neisseria meningitidis serogroup B

Fructose-1,6-bisphosphate aldolase (FBA) is a classical cytoplasmic glycolytic enzyme which, despite lacking a predicted signal peptide, has been demonstrated to be expressed and transported to the surface of numerous Gram-positive bacteria and shown to interact with host molecules and perform non-glycolytic biological functions. Genome-based studies have also demonstrated that the glycolytic pathway appears to be...

Author(s): Sarfraz A. Tunio, Neil J. Oldfield, Karl G. Wooldridge and David P. J. Turner

July 2012

In vitro propagation of Ceropegia thwaitesii Hook- an endemic species of Western Ghats of Tamil Nadu, India

In vitro propagation of Ceropegia thwaitesii Hook (Asclepiadaceae), an endemic plant species of Western Ghats, Tamil Nadu (India), was carried out. Auxiliary bud explants were cultured on Murashige and Skoog (MS) medium fortified with cytokinins (KIN), 6-benzyl adenine (BA), 2-isopentenyladenine (2-iP) and thidiazuron (TDZ) in various concentrations and in combination with auxins indole-3-butyric...

Author(s): S. Muthukrishnan , J. H. Franklin Benjamin, M. Muthukumar, N. Ahamed sherif, T. Senthil kumar, M. V. Rao

July 2012

Characterization of water uptake and distribution in chickpea (Cicer arietinum L.) seeds during germination by NMR spectroscopy

Experiments were conducted to characterize the changes in water status during imbibition by nuclear magnetic resonance (NMR) spectroscopy in chickpea seeds exposed to static magnetic fields of 100 mT for 1 h. Water uptake during seed germination showed three phases with rapid initial hydration phase I, followed by lag phase II and steady hydration phase III. Comparative analysis of the hydration pattern showed that...

Author(s): Ananta Vashisth, D.K. Joshi and Ravender Singh

July 2012

Genotype variation in grain yield response to basal N fertilizer supply among different rice cultivars

Considering the great amount of basal N fertilizer but lower uptake ability at rice seedling, it was essential to increase the N use efficiency of basal fertilizer and reduce N pollution. So, a field experiment was conducted at Wuxi, China, under non-basal N and basal N fertilizer conditions, to identify the variation of grain yield response to basal fertilizer among 199 rice varieties with different genetic background,...

Author(s): Shou-Ping Zhao, Xue-Qiang Zhao and Wei-Ming Shi

July 2012

Heavy metals burden of Keenjhar Lake, District Thatta, Sindh, Pakistan

Detection of heavy metals (HMs) content from Keenjhar Lake water was carried out monthly from January to December, 2003. Zinc, chromium, copper, iron, manganese, nickel and cadmium were analyzed by dual mode of analytical methods flame atomic absorption spectrometry and electrothermal atomic absorption spectrometry (FAAS and ETAAS) by multi element standard solution. The concentrations of zinc, chromium, copper, iron,...

Author(s): Lashari, K. H., Sahato, G. A., Korai, A. L., S. Habib Naqvi, Palh, Z. A.  and Urooj, N.

July 2012

Production of phytate-hydrolyzing enzymes by thermophilic moulds

138 isolates of thermophilic/thermotolerant moulds were isolated from soil, straw and compost samples, collected from various regions of India. Among the thermophilic fungal isolates screened for the secretion of phytase (phytate-hydrolyzing enzyme),Sporotrichum thermophile BJTLR50 produced a very high enzyme titre at pH 5.0, 45°C and 250 rpm in 5 days with an inoculum level of 1 × 107 spores per 50...

Author(s): Bijender Singh, and T. Satyanarayana

July 2012

Effects of various additives on antioxidant and antimicrobial effectiveness in emulsion-type sausages

We investigated the effects of rosemary extract (RE), α-tocopherol (AT) and chitosan (CH) added individually or in combination as compared with butylated hydroxyanisole (BHA) on microbiological parameters [total viable count (TVC), lactic acid bacteria (LAB), enterobacteria (ENB), pseudomonas bacteria (PSY)], pH and lipid oxidation of emulsion-type sausages stored for 28 days at 4°C. TVC, LAB, ENB, and...

Author(s): Y. J. Kim, C.M. Kim, J. H. Choi and I. H. Choi

July 2012

Survivability of probiotics in symbiotic low fat buffalo milk yogurt

In present study, symbiotic low fat buffalo milk yogurt prototypes (plain and blueberry) were developed using a commercial starter containing probiotics. Samples were analyzed for physicochemical and microbiological properties, and the survivability of probiotics during 10 weeks of storage. Gross composition results were: total solids 11.60 ± 0.58 and 17.12 ± 0.36%, ash 0.82 ± 0.06 and 0.78 ±...

Author(s): Xue Han, Lanwei Zhang and M. R. Guo

July 2012

Response surface methodology for optimization of the treatment of textile wastewater by a novel bacterial consortium: Enzymes and metabolites characterization

Textile wastewater (TWW) causes many environmental problems, and colored substances must be removed from it before their discharge into the environment. An optimization process was analyzed using custom response surface methodology (RSM). In the present study, the effect of different combinations of eight strains was studied using equilateral triangle diagram (Minitab 14.0) and mixture experimental design to assess...

Author(s): Lamia Ayed#, Imen Ksibi#, Abdelkarim Cheref and Amina Bakhrouf

July 2012

Enzymatic epoxidation of biodiesel optimized by response surface methodology

During the enzymatic epoxidation of biodiesel, stearic acid was selected as oxygen carrier. Enzyme screening and the load of stearic acid were investigated. The effects of four main reaction conditions including reaction time, temperature, enzyme load, and mole ratio of H2O2/C=C-bonds on the epoxy oxygen group content (EOC) of epoxidized biodiesel were analyzed. Response surface methodology (RSM) was employed to study...

Author(s): Hao Lu, Shangde Sun, Yanlan Bi and Guolong Yang

July 2012

Pathological study of parasitism in racing pigeons: An indication of its effects on community health

Several non-official reports from different clinics, governmental veterinary head-quarters and bird keepers indicate that most of the young pigeons die with suspicious infection to parasites. In addition, the pigeon owners were complaining of skin itching on their head. Thus, this research was conducted to determine the pathological study of parasitism in racing pigeons with an indication of its effects on...

Author(s): Ali Mohammad Bahrami, Ali Louei Monfared and Mostafa Razmjoo

July 2012

Comparison of broiler performance, carcass yields and intestinal microflora when fed diets containing transgenic (Mon-40-3-2) and conventional soybean meal

This study was conducted to analyze the effects of transgenic glyphosate-tolerant soybeans on the performance, carcass yields and intestinal microflora of broiler chickens. Three hundred and sixty one-day-old Abor Aerec broilers were randomly divided into two dietary treatments, adding genetically modified (GM) glyphosate-tolerant soybean meal or conventional soybean meal, respectively. Broiler...

Author(s): Jianzhuang Tan, Shasha Liu Zhe Sun Hongfu Zhang, Yongwei Wang and Dan Liu