African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 24 May 2012; 11(42)

May 2012

Association of polymorphisms in the Pit-1 intron 5 with body measurements in Chinese Cattle

  The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to identify polymorphism in the Pit-1 intron 5. One single nucleotide polymorphism, Pit1I5, was found in intron 5. The frequencies of allele A of Nanyang cattle (NY), Qinchuan cattle (QC), Jiaxianhong cattle (JX),...

Author(s): Ligang Tang, Dongying Yang, Wuqing Ouyang, Liangzhi Zhang, Xianyong Lan, Chunlei Zhang, Runfeng Zhang, Ailing Zhang and Li Zhang, Hong Chen

May 2012

The sequence-related amplified polymorphism (SRAP) markers linked to the color around the stone (Cs) locus of peach fruit

  The distribution and concentration of anthocyanin, a major subgroup of the flavonoids and important determinants of peach fruit flesh color, is regulated by several genes. In the international reference Prunus map, the locus of flesh color around the stone (Cs) is located on linkage group LG3. This report describes the development of a sequence-related amplified polymorphism (SRAP) marker linked to...

Author(s): Ji-cheng Han, Guo-jian Liu, Rui-feng Chang and Xin-zhong Zhang,

May 2012

Molecular cloning, heterogenous expression and the induction profiles after organophosphate phoxim exposure of the carboxylesterase Bmae33 in the silkworm, Bombyx mori

  Carboxylesterases (COEs) are a multifunctional supergene family and some of them play important roles in hydrolyzing a wide variety of carboxylic acid esters. In insects, COEs are related to xenobiotic detoxification, pheromone degradation and developmental regulation. In the present study, one silkworm COE Bmae33 gene, an ortholog to other Lepidopteran odorant-degrading esterases, was cloned...

Author(s): Dan-Dan Feng, Wei Yang, Wen-Juan Yang, Quan-You Yu and Ze Zhang,

May 2012

An efficient method for production of Tunisian melon (Cucumis melo L. cultivars Maazoun and Beji) by somatic embryogenesis

  Somatic embryogenesis in two Cucumis melo L. cultivars, Maazoun and Beji, mainly cultivated in the Center and the North of Tunisia was induced from in vitro cultures derived from cotyledons, hypocotyls and zygotic embryos. The initiation of embryos was influenced by the hormonal composition of the media, the source of explant and the genotype. The three explants were cultured on...

Author(s): RHIMI Awatef and BOUSSAID Mohamed

May 2012

Exogenous ascorbic acid increases resistance to salt of Silybum marianum (L.)

  Salinity stress has negative effects on agricultural yield throughout the world, affecting production whether it is for subsistence or economic gain. This study investigates the inductive role of vitamin C and its application mode in mitigating the detrimental effects of irrigation with diluted (10, 20 and 30 %) NaCl + water on Silybum marianumL. plants. The results show that 10% of salt water exhibited...

Author(s): Banu Aytül Ekmekçi and Meltem Karaman

May 2012

Optimisation of wort production from rice malt using enzymes and barley malt

  Commercially, rice malt has never been successfully used in brewing because of its low free a-amino nitrogen (FAN) content. This study was designed to optimise rice malt replacement for barley malt in wort production and to improve FAN by adding a-amylase and protease. The response surface methodology (RSM) through face central composite design (CCD) was selected to examine the effects of the four...

Author(s): Artit Kongkaew, Ulaiwan Usansa, and Chokchai Wanapu

May 2012

First report of Anthracnose/collar rot caused by Colletotrichum dematium on statice (Limonium sinuatum) in Pakistan

  During the month of March 2011, the incidence of anthracnose caused byColletotrichum dematium on statice grown as ornamental plants at Ayub Agricultural Research Institute Faisalabad, Pakistan, was recorded. The characteristic symptoms were first, brownish spots on leaves and branches, and then drying of branches starting from plant tips down to the collar region, causing collar rots and leading to the...

Author(s): Abdul Rehman, Saira Mehboob, Chrys Akem, Asia Batool and M. Idrees

May 2012

Molecular detection of Cylindrocarpon destructans in infected Chinese ginseng roots and soil

  Ginseng (Panax ginseng C.A. Meyer) is one of the most important medicinal plants in China, but its yields are often reduced by a variety of root pathogens. The root rot of ginseng is a destructive soil-borne disease caused by Cylindrocarpon destructans(teleomorph: Neonectria radicicola). A species-specific polymerase chain reaction (PCR) assay was developed for rapid detection of C....

Author(s): Junfan Fu, Jiaman Sun, Rujun Zhou and Xuerui Yan

May 2012

Reproducible in vitro regeneration system for purifying sugarcane clones

  Genome purification of a selected clone of sugarcane is the key to developing homogenous lines. Generally, regenerated plants after transformation are heterogeneous at genome level, and several successive rounds of selection on antibiotic-containing medium and regeneration cycles are required to purify the genome to develop a homogenous population. Sugarcane is a vegetatively propagated plant and hence it...

Author(s): Ghulam Mustafa, and Muhammad Sarwar Khan,

May 2012

In vitro mass propagation of an epiphytic orchid,Dendrobium primulinum Lindl. through shoot tip culture

  The present study develops a protocol for rapid in vitro micropropagation of a critically endangered and floriculturally most important epiphytic orchid, Dendrobium primulinum Lindl. through the culture of small shoot tip explants (0.3 to 0.5mm) derived from in vitro grown seedlings. The shoot tip explants cultured on solidified Murashige and Skoog (MS) basal...

Author(s): Bijaya Pant and Deepa Thapa

May 2012

In vitro regeneration of Gerbera jamesonii cv. Sunglow

  This study demonstrates a protocol for in vitro regeneration of Gerbera jamesonii cv. ‘Sunglow’ developed by culturing leaf segments on Murashige and Skoog (MS)medium supplemented with 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1 indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acid (2,4-D) and 1.0, 2.0, 3.0, 4.0 and 5.0 mg...

Author(s):   Khaliqa Shabbir, Touqeer Ahmad, Ishfaq Ahmad Hafiz, Azhar Hussain, Nadeem Akhtar Abbasi and Jamil Ahmad

May 2012

In vitro control of Alternaria citri using antifungal potentials of Trichoderma species

  The antifungal potential of five species of Trichoderma viz., Trichoderma viride,Trichoderma aureoviride, Trichoderma reesei, Trichoderma koningii and Trichoderma harzianum was investigated in vitro against Alternaria citri, the causal agent of the black rot disease on a broad range of citrus cultivars. Cultural filtrates of Trichodermaspecies were obtained by...

Author(s): Asma Murtaza, Shazia Shafique, Tehmina Anjum and Sobiya Shafique

May 2012

Biogenic volatile compounds of activated sludge and their application for metal bioremediation

  Heavy metals pollution is nowadays one of the most important environmental concerns. This paper illustrates the employment of the biogenic volatile compounds generated during the aerobic growth of activated sludge on raw domestic wastewater for heavy metals removal. Most of the tested metals even as individual or mixed metal species (Co, Cu, Cd, Fe, Hg, Ni, Mn, Pb and Zn) were potentially transformed into...

Author(s): Ashraf M. Essa, Eman S. Abd-Alsalam and Refat M. Ali

May 2012

Physico-chemical properties of oil from some selected underutilized oil seeds available for biodiesel preparation

  In the past few decades, there has been increasing demand for reduction on the reliance on fossil fuels. As a result, there is an increasing search for renewable resources for biodiesel production. In this study, the feasibility of using some selected tropical seeds: palm kernel, breadfruit, groundnut, bambara groundnut, pumpkin, African oil bean, melon, sesame, coconut, soybean, Cucumeropsis manii,...

Author(s): Sabinus Oscar O. EZE

May 2012

In vitro susceptibility testing of Yersinia species to eight plant extracts and three natural antimicrobial agents

  The antimicrobial effects of the ethanolic and aqueous extracts of the leaves ofCymbopogon citratus (DC) Stapf, Acalypha hispida Forsk, Vernonia amygdalina Del,Occimum gratissimum L., Sida acuta Burm, F., Coffea arabica L., seeds of Carica papaya L., fruit juice of Citrus aurantifolia (Christim) Swingle, wild honey, processed honey...

Author(s): Owhe-Ureghe, U. B., Ogiehor, S. I. and Ehwarieme, D. A.

May 2012

Studies on amylase activity of an amylolytic bacterium isolated from estuarine soil

  Diverse microscopic, macroscopic and biochemical analysis of a starch degrading amylolytic bacterial strain isolated from the soil sample of Rajakkamangalam estuary, Kanyakumari district, Tamil Nadu, India, revealed its identity to the genus Bacillus.Maximum growth was observed at 12 h when the bacteria was cultured in minimal agar media with 2% starch maintained for 12 h, at 37°C and pH 5.5, 7.5 and...

Author(s): A. Punitha, M. R. Basil-Rose, J. Albino Wins, S. Prakash Shoba and S. Mary Mettilda Bai

May 2012

Antibacterial activities of extracts of leaf, fruit, seed and bark of Phoenix dactylifera

  The antibacterial activities of different parts of local Phoenix dactylifera were investigated in vitro. Dried leaf, fruit, seed and tree bark were extracted with water, methanol and acetone. Antibacterial property of the extracts was evaluated againstStaphylococcus aureus, Streptococcus pyogenes, Escherichia coli andPseudomonas aeruginosa using the disc diffusion method. Overall...

Author(s): Sooad Al-daihan and Ramesa Shafi Bhat

May 2012

An alpha-glucosidase inhibitory activity of thermostable lectin protein from Archidendron jiringa Nielsen seeds

  Inhibitors of α-glucosidase from natural resources that inhibit the digestion of carbohydrate polymers into monosaccharides in the gut are used in the treatment of insulin-independent diabetes mellitus type 2. Archidendron jiringa belongs to pea family of leguminous plants, some of which are a source of interesting bioactivities, including α-glucosidase inhibitory (GI) activity. A novel...

Author(s): Panadda Virounbounyapat, Aphichart Karnchanatat and Polkit Sangvanich

May 2012

Effect of varied quantities of zeolite on the reduction of polycyclic aromatic hydrocarbons in tobacco smoke

  This research was carried out to determine the possibility of total and selective reduction of polycyclic aromatic hydrocarbons (PAHs) content in cigarette smoke by applying different amounts of zeolite directly to the cigarette blend. Zeolite catalysts CuZSM-5 were applied in the form of suspension to the cut tobacco blend in the quantities of 1 and 3%. After the equalization of the blend, the cigarettes...

Author(s): Vesna Radojičić, Sladjana Alagić, Borivoj Adnadjević and Abduladhim M. Maktouf

May 2012

Antioxidant effects and phytochemical analysis of crude and chromatographic fractions obtained from Eucalyptus globulus bark

  Eucalyptus globulus is extremely used in traditional medicine in Algeria and its leaf extract is included as one of the antioxidant in the list of existing food additives in Japan. In the present study, the crude extract from bark of this plant and its fractions obtained by Sephadex LH-20 column were studied to quantify their levels of phenolic compounds and their antioxidant activity (ferric...

Author(s): Boulekbache-Makhlouf Lila, Slimani Sakina and Madani Khodir