African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 25 September 2012; 11(77)

September 2012

Genetic variation in the population of three Polish cattle breeds included into the programme of genetic resources protection and Holstein-Friesian breed, estimation on the basis of polymorphism of 24 microsatellite DNA sequences

The study focused on determining and characterizing genetic variation of three Polish cattle breeds [Whiteback (WB), Polish Red (PR) and Polish Black-and-White (PBW)] included in the programme of genetic resources conservation. The obtained results were related to the genetic variation within the Holstein-Friesian (PHF) breed of Black-and-White variety. Overall, 214 alleles were identified within four examined breeds,...

Author(s): Wioletta Sawicka-Zugaj and Zygmunt Litwińczuk

September 2012

Molecular cloning and characterization of an acyl-ACP thioesterase gene (AhFatB1) from allotetraploid peanut (Arachis hypogaea L.)

Acyl-acyl carrier protein (ACP) thioesterase is a nuclear encoded plastid localized enzyme which plays an essential role in chain termination during de novo fatty acid synthesis in plant. FatB genes coding for this enzyme from a variety of plant species have been isolated and characterized. However, there are few reports on such genes in peanut (Arachis hypogaea), an important edible and oilseed crop. In this...

Author(s): Wen Qigen, Lei Yong, Huang Jiaquan, Jiang Huifang and Liao Boshou

September 2012

Prevalence of viruses infecting cowpea in Uganda and their molecular detection

The main areas for cowpea cultivation in Uganda were surveyed in June and October 2006 for viruses affecting the crop. Seed and leaf samples from symptomatic and asymptomatic plants were collected from farmers’ fields and analysed for infecting viruses using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). The viruses detected in the leaf and seed samples were: cucumber mosaic...

Author(s): Amayo, R., Arinaitwe, A. B., Mukasa, S. B., Tusiime, G., Kyamanywa, S., Rubaihayo, P. R. and Edema, R.

September 2012

Effects of calcium paste as a seed coat on growth, yield and enzymatic activities in NaCl stressed-pea plants

The present study was conducted to study the effect of coating the seeds with calcium paste before sowing, on plant growth, yield, the contents of some antioxidants and the activities of carbonic anhydrase and nitrate reductase in the Pisum sativum L. leaves under the influence of NaCl stress. NaCl stress reduced plant growth, photosynthetic pigment levels, ascorbic acid and calcium contents, and the...

Author(s): Saad M. Howladar and Mostafa M. Rady

September 2012

Lead contamination in soil and vegetation of areas surrounding different mining activity zones in Kerman Province of Iran

Lead (Pb) is a toxic element that was used for many years in products found in our environment. One of The most serious sources that Pb can be emitted into theenvironment is industrial and mining activities. A study was carried out, samples collections from soil and wastewater in Ghanat Marvan lead mine, Tarz lead mine, Shahid Bahonar Copper Industries Company (CSP), Industrial zone No.1 and Sarcheshmeh Copper...

Author(s): Salari Hassan, Oloumi Hakimeh, Afzali Daryoush and Ahmadi Mosavi Effatosadat

September 2012

Screening of potential biosurfactant-producing bacteria isolated from seawater biofilm

Seawater represents a specific environment harboring complex bacterial community which is adapted to harsh conditions. Hence, biosurfactant produced by these bacteria under these conditions have interesting proprieties. The screening of biosurfactant producing strains isolated from seawater biofilm was investigated. Specific media, which have been reported to induce biosurfactant production, were used to prepare...

Author(s): Saïd Ben Hamed, Latifa Smii, Abdeljelil Ghram and Abderrazak Maaroufi

September 2012

A full-scale biological aerated filtration system application in the treatment of paints industry wastewater

This study was carried out to evaluate an existing treatment technology for wastewater treatment unit from a paint production factory. Biological aerated filter is a good technique for reducing land used in the purpose of wastewater treatment. Influent discharged from this factory ranged between 50–120 m3/day with an average of 70 m3/day. This unit composed of primary and secondary sedimentation tanks, gravel...

Author(s): IBRAHIM E. Mousa

September 2012

Biodegradation of used lubricating and diesel oils by a new yeast strain Candida viswanathii KA-2011

Disposal of the automotive lubricating oil after usage can cause environmental hazards. This study aimed to isolate a microbial isolate is that able to biodegrade lubricating oil effectively. A new yeast strain, identified by 18S-rRNA gene sequencing as Candida viswanathii KA-201l, was isolated from used lubricating oil, showed high biodegradation efficiency for different used lubricating oils. Capability...

Author(s): Khaled M. A. Ramadan, Abdel Azeiz A. Z., Hassanien S. E. and Eissa H. F.,

September 2012

Isolation, identification and screening of potential cellulase-free xylanase producing fungi and its production

In order to isolate cellulase-free xylanase producing fungi, screening and isolation was done using composting soil as microbial source. Eight fungal species were selected for further study based on clearing zones formation on agar media containing covalently linked xylan with dye cibacron brilliant red-3BA. Both solid state and submerged fermentations were done with eight fungal isolates to identify strain that could...

Author(s): Rajashri D. Kamble and Anandrao R. Jadhav

September 2012

Antioxidant and tyrosinase inhibitor from Leucaena leucocephala

The experimental design is divided into two parts: chemical analysis and bioactive assay. One antioxidant lupeol (4) and one inhibition of tyrosinase pheophorbide a methyl ester (7) were identified in Leucaena leucocephala. Both showed effective 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity compared with vitamin C, and mushroom tyrosinase compared with kojic acid. These results suggest that these...

Author(s): Hsing-Tan Li, Syun-Wun Ruan, Jin-Cherng Huang, Hsin-Liang Chen and Chung-Yi Chen

September 2012

Effects of Platycodon grandiflorum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

Benign prostatic hyperplasia (BPH) is highly prevalent in the male population over the age of 60 years, manifesting as prostatic enlargement and distinctive changes in tissue histomorphology. In this study, we investigated whether a Platycodon grandiflorum methanolic extract (PGME) improved BPH in a testosterone propionate (TP)-induced model of BPH in rats. Castration was performed via the scrotal route under...

Author(s): Mee-Young Lee, In-Sik Shin, Nam-Hun Lee,  Hyekyung Ha, Jung-Hoon Kim, Chang-Seob Seo and  Hyeun-Kyoo Shin

September 2012

Bioassay and enzymatic comparison of six entomopathogenic fungal isolates for virulence or toxicity against green peach aphids Myzus persicae

Myzus persicae is an economically important pest infecting more than 40 families of crops throughout the world. Six entomopathogenic fungal isolates, three each ofBeauveria bassiana and Verticillium lecanii, were screened for pathogenicity test against the M. persicae to select high virulent isolate with the most suitable application and to determine the role of individual enzyme in its...

Author(s): Sehroon Khan, Lihua Guo, HuaiXing Shi, Mahmut Mijit and Dewen Qiu

September 2012

Bioaccumulation of heavy metals in water, sediment and fish (Oreochromis niloticus and Clarias anguillaris), in Rosetta branch of the River Nile, Egypt

Rosetta Branch of the River Nile is exposed to high input of agricultural drainage water, sewage and industrial water that affect living organisms like fish. In the present study, some heavy metals such as iron, copper, lead, cadmium and zinc (Fe, Cu, Pb, Cd and Zn) were seasonally determined in some tissues of tilapia (Oreochromis niloticus) and catfish (Clarias anguillaris), water sediment and in Rosetta branch of...

Author(s): Hany M. Yehia and Elwoa Sh. Sebaee

September 2012

Purification and characterization of recombinant glucose dehydrogenase isolated from a hyperthermophilic Sulfolobus-like bacterium

This study aimed to clone and characterize a thermal resistant glucose dehydrogenase (GDH) and investigate its clinical potential. A Sulfolobus-like thermophilic microbe was first isolated from a hot spring in Taitung, Chihpen County, Taiwan. The gene encoding GDH was cloned from the bacterium and expressed inEscherichia coli. The molecular weight of the enzyme was found to be approximately 39,000 kDa. The enzyme...

Author(s): Liang-Jung Chien, Yu-Ting Duan and Ni-Yin Yu

September 2012

Molecular characterization of two populations of catfish Clarias batrachus L. using random amplified polymorphic DNA (RAPD) markers

Studies were undertaken to identify genetic relationship and diversity of Clarias batrachus L. populations collected from hatchery and wild stocks through random amplified polymorphic DNA (RAPD). 16 decamer primers were used for screening, out of which five produced amplicons. A total of 1376 RAPD bands ranging from 0.2 to 1.36 kb were amplified using five selected primers. The number of amplification products...

Author(s): Mohd. Danish, I. J. Singh, Priyanka Giri and C. P. Singh

September 2012

The effects of the seeds of Galeopsis ladanum on fattening performance in quails and occurrence of rhabdomyolysis in rats

This study was conducted to investigate the effect of Galeopsis ladanum seeds on rhabdomyolysis occurrence in rats as an animal model and fattening performance of quails. Control group was fed with basal diet without G. ladanum seeds. Diets of treatment groups contained 2 or 4% of G. ladanum seeds, respectively. All animals, quails and rats were fed individually. At the end of the study, 2...

Author(s): Mustafa SALMAN, Ö. Hakan MUÄžLALI, M. Yavuz GÜLBAHAR, Yasemin ÖZDENER and Zehra SELÇUK

September 2012

Non-intact zona improves development of murine preimplantation embryos transfected by an adenovirus vector

The present study explored whether embryos could be transfected by the adenovirus-vector if the zona pellucida (ZP) was not completely removed. An adenovirus vector with green fluorescent protein (pAd-GFP) was used to transfect mouse non-intact zona zygotes (following partial removal of the ZP induced by pronase), zona-free and zona-intact embryos. Non-intact zona and zona-free embryos expressed GFP (confirmed with...

Author(s): Hong-wei Zhao, Qing-wang Li, Zeng-sheng Han, Da-wei Gao, Kun Li, Yong Peng and Zhi-wei Liu