June 2013
Aquaculture-oriented genetic researches in abalone: Current status and future perspective
Basic genetic and cytogenetic information including polymorphic DNA markers, chromosomes and genome size was summarized to get insights into phylogenetic or systematic relationship among abalone species belonging to the genus Haliotis. Hybridization, triploidization and genetic mapping were also briefly reviewed as aquaculture-oriented genetic techniques to improve growth and other commercially important...
June 2013
Simple sequence repeat (SSR)-based genetic variability among peanut genotypes different in specific leaf weight and relative water content
The objective of this study was to compare if simple sequence repeat (SSR) markers could correctly identify peanut genotypes with difference in specific leaf weight (SLW) and relative water content (RWC). Four peanut genotypes and two water regimes (FC and 1/3 available water; 1/3 AW) were arranged in factorial randomized complete block design with six replications. The data were recorded for...
June 2013
RAPD-PCR molecular analysis of the threatened Cabrera’s vole populations in the Iberian Peninsula
Optimal management and conservation programs of the threatened Cabrera’s vole require investigating potential molecular genetic markers in the genomic background, if the few remaining fragile populations are to be conserved. A collection of 30 Cabrera’s vole representing four populations in Spain and Portugal was characterized by 134 RAPD-PCR markers. Molecular analyses did not detect low level of...
June 2013
Genetic diversity analysis of rice cultivars from various origins using simple sequence repeat (SSR) markers
Genetic diversity is of paramount importance for the success of any plant breeding program. An experiment was conducted to assess the extent of genetic diversity and similarity of 24 rice cultivars from various origins using 29 simple sequence repeat (SSR) markers. A total of 144 alleles were detected at the 29 SSR primer pairs evaluated in the 24 cultivars. The number of alleles per loci ranged from 3 to 8...
June 2013
Genetic diversity of Pyrenophora tritici-repentis in Algeria as revealed by amplified fragement length polymorphism (AFLP) analysis
Tan spot caused by Pyrenophora tritici-repentis is a major wheat disease. DNA of 61 isolates of P. tritici-repentis from different cereal growing areas in Algeria were analyzed using amplified fragment length polymorphism (AFLP) in order to study the genetic diversity among this population. Initially, 78 primer combinations were tested, of which 12 were selected and applied to...
June 2013
A simple, fast and efficient method for cloning blunt DNA fragments
To increase a recombinant ratio of cloning blunt DNA fragments, for example, polymerase chain reaction (PCR) products, a vector DNA is usually digested by a blunt-end producing restriction endonuclease and then dephosphorylated in order to avoid self-ligation of the linearized vector. In this paper, we described a simple method to directly ligate a blunt DNA fragment to a non-dephosphorylated vector with a...
June 2013
Determination and expression of genes for resistance to blast (Magnaporthe oryza) in Basmati and non-Basmati indica rices (Oryza sativa L.)
One hundred and twenty two (122) genotypes of Basmati and non-Basmati Indicarice genotypes were evaluated for expression of resistance against blast disease under induced epiphytotic conditions. Disease severity (%) and area under disease progress curve (AUDPC) parameters were used for screening the blast resistance. Only 13 genotypes expressed resistance against the blast disease. Nine...
June 2013
Isolation and characterization of beneficial indigenous endophytic bacteria for plant growth promoting activity in Molelwane Farm, Mafikeng, South Africa
Plant-associated bacteria that live inside plant tissues without causing any damage to plants are defined as endophytic bacteria. The present study was carried out to analyze the phenotypic and genotypic diversity of endophytic bacteria associated withAmaranthus hybridus, Solanum lycopersicum and Cucurbita maxima. A total of 50 bacteria were isolated from the roots of the plants. All the isolates...
June 2013
Decolorization of the dye reactive black 5 using Fenton oxidation
Study on the effect of Fenton process on decolorization and mineralization of RB-5 in aqueous solution is presented in this work. Batch experiments were carried out to find the optimal operational conditions including pH, temperature, H2O2 dosage, FeSO4dosage and RB-5 concentration at low cost. Degradation of RB-5 dye as an organic pollutant was monitored based on decolorization and mineralization...
June 2013
Isolation and phylogenetic analysis of zinc resistant Acinetobacter sp. and its potential for bioremediation
In this study, a gram negative, non- motile short rod-shaped and heavy metal resistant bacterial strain was isolated from effluents. The optimum temperature and pH for the growth of the strain were 33°C and 7, respectively. The minimum inhibitory concentrations (MICs) of zinc (Zn), copper (Cu), chromium (Cr) and mercury (Hg) against the isolate were determined. The isolate showed MICs of 5, 4, 3 and...
June 2013
Surveillance of multidrug resistant bacteria pathogens from female infertility cases
Antibiotic resistance is a public health problem of increasing magnitude. Female reproductive system is vulnerable to a number of diseases. Diseased condition results in infertility, menstrual irregularity, pregnancy loss, and in association with pregnancy, morbidity to both the mother and child increases. In the present work, the bacteria pathogens were isolated from the endometrial sample of 50 female...
June 2013
A marine bacterium, Oceanobacillus sp. Pinky, isolated from Algoa Bay sediment produces a thermostable glycoprotein flocculant
In this study, we report on the bioflocculant production potential of an Oceanobacillussp. isolated from the marine sediments of Algoa Bay. The bacteria produced an extracellular bioflocculant optimally in the presence of sodium carbonate as source of carbon with flocculating activity of about 95.5%. Other optimal culture conditions included: tryptone as nitrogen source (flocculating activity, 84.5%);...
June 2013
Effect of micro-organism and particle size on fermentation of sorghum and maize for poultry feed
A study was conducted to investigate the effect of particle size and micro-organism on fermentation of sorghum and maize for poultry feed. Sorghum (Sorghum bicolourL. Moench) and maize were milled in a hammer mill and separated into coarse, medium, fine and very fine particles sizes with a stack of sieves of apertures 2.5 mm, 850 µm and 500 µm, from the first to the last sieve and ending in a...
June 2013
Growth and extracellular enzyme production by microorganisms isolated from Ugba - an indigenous Nigerian fermented condiment
Studies were conducted to isolate and identify the fermenting microorganisms of ‘Ugba’, an indigenous Nigerian fermented food condiment. The isolated microorganisms were screened for amylase, protease and lipase production, the activity and specific activity of the enzymes were also determined. The effect of pH and temperature on the activity of the enzymes was also studied. The following groups...
June 2013
Vitamins and phytochemical contents in four leafy vegetables subjected to different processing methods
Vitamin and phytochemical contents of the leaves of Vernonia amygdalina, GnetumAfricanum, Gongronema latifolium and Ocimum gratissimum subjected to different processing methods were investigated. Processing treatments employed include fresh milling, sun drying, oven drying, steaming and a combination of these. The vitamins evaluated include vitamins A, B1, B2, B3, B6 and E while...
June 2013
Nutrients composition of calyces and seeds of three Roselle (Hibiscus sabdariffa L.) ecotypes from Niger
The chemical composition of calyces and seeds of three ecotypes of Roselle from Niger was compared. The results indicate that calcium (Ca), potassium (K), sodium (Na), magnesium (Mg) and protein contents in calyces are significantly different (P<0.005) among ecotypes. The highest concentrations of K, Na, Mg and protein in calyces were recorded for ecotype E7 (35.66, 3.40, 6.01 and 101 mg/g d.w.,...
June 2013
Characterization of element and mineral content in Artemisia annua and Camellia sinensis leaves by handheld X-ray fluorescence
Tea infusion is the most frequently worldwide consumed beverage next to water, with about 20 billion cups consumed daily. Artemisia annua leaves contain comparable levels of nutrients and mineral elements (dry matter basis) to many marketed tea (Camellia sinensis) leading us to suspect that this crop could also serve as an alternative source of nutrients for humans. Analyzer moveable X-ray...
June 2013
Bioinformatics and phylogenetic analysis of human Tp73 gene
The Tp73 gene encoding p73 protein belongs to the Tp53 gene family and it functions in the initiation of cell-cycle arrest or apoptosis and also involves in regulating a series of pathways including breast cancer, neuroblastoma and cholorectal cancer. New discoveries about the control and function of p73 are still in progress and it is hoped to develop better diagnostics and...
June 2013
Effect of dietary crude protein level on the performance and apparent digestibility of Iranian Saanen kids
The effects of increasing dietary levels of crude protein (CP) on growth, feed intake, feed efficiency and nutrient apparent digestibility on Iranian Saanen kids were studied. 24 Iranian Saanen weaned kids who were 86 ± 3 days old with live weight of 9 ± 03 kg were used in a completely randomized design. There were three treatments (n = 8 kids per treatment). The dietary treatments contained 35%...
June 2013
Microencapsulation of probiotic bacteria: Pharmaceutical microencapsulation for synbiotic application
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