African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 28 June 2012; 11(52)

October 2013

Mutated N-ras does not induce p19arf in CO25 cell line

The mouse cell line (CO25) used in this study was transfected with a glucocorticoid inducible mutated human N-ras oncogene under transcriptional control of the steroid-sensitive promoter of the mouse mammary tumors virus long terminal repeat MMTV-LTR. This study was aimed to investigate the expression of p19arf and MDM2 genes under the effect of N-ras oncogene induction and to invent the role of p19arf, MDM2...

Author(s): amel A. Saleh, Mohammed Ali Alshehri, Mohammed Yahya Al-faifi and Hulya Sivas

October 2013

Identification of random amplified polymorphic DNA (RAPD) marker of Ph-3 gene for late blight resistance in tomato

Late blight is a highly destructive disease of tomato worldwide. Host resistance is the most effective method for disease control. The application of molecular markers is an efficient way to identify host resistance for breeding programs. In this study, bulked segregant analysis (BSA) was used to search for random amplified polymorphic DNA (RAPD) markers linked to the late blight resistance gene Ph-3, using an...

Author(s): Qingdao Zou, Haitao Li, Zijun Zhang, Shuwen Lu, Yongcheng Wang, Guodong Yang, Xinyu Zhang and Yipeng Qiu

October 2013

Effect of cadmium stress on antioxidative enzymes during the germination of Serbian spruce [Picea omorika (Panč.) Purkynĕ]

When considering the effect of heavy metals on trees generally and on forest ecosystems especially, importance is given to their influence on seed germination in metal polluted soil. There is insufficient data on this subject, especially when conifers are concerned. In this work, the influence of high cadmium concentrations on percentage germination, specific activities and isoenzyme patterns of catalase (CAT),...

Author(s): Olivera Prodanović, Radivoje Prodanović, Jelena Bogdanović  Pristov, Aleksandra Mitrović and Ksenija Radotić

October 2013

1H-NMR metabolomics: Profiling method for a rapid and efficient screening of transgenic plants

Metabolomics-based approaches are methods of choice for studying changes in fruit composition induced by environmental or genetic modulation of biochemical pathways in the fruit. Owing to enzyme redundancy and high plasticity of the metabolic network, transgenic alteration of the activity of the enzymes from the central metabolism very often results in only slight modifications of the fruit composition. In order to...

Author(s): Yosra Ben Akal-Ben Fatma, Katia Pianelli, Martine Dieuaide-Noubhani, Aline Le Menn, Catherine Deborde, Mickaël Maucourt, Marie-Hélène Andrieu, Dominique Rolin, Christophe Rothan, Annick Moing, Sadok Bouzid and  Cécile Cabasson,.

October 2013

Effects of arbuscular mycorrhizal fungi on resistance to Phytophthora parasitica of citrus seedlings and on growth of Thai honey tangerine scions on citrus rootstocks

Thai honey tangerine (Sainamphueng tangerine) is generally grown by grafting on rootstocks of another variety of tangerine or citrus species which may differ in their reaction to beneficial and pathogenic soil organisms. The objectives of this study were to evaluate responses to arbuscular mycorrhizal (AM) fungi and Phytophthora parasitica of different citrus genotypes and the effect of AM...

Author(s): Somchit Youpensuk, Wonwinee Piwpueak and Benjavan Rerkasem

October 2013

Baseline information on using fermented crude extracts from Cucumis africanus fruit for suppression of Meloidogyne incognita and improving growth of tomato plants

Bio-pesticides, when used as a post-planting pesticide, are limited by their potential ability to suppress the pest and their degree of phytotoxicity. Baseline information on the suitability of fermented crude extracts (FCE) of Cucumis africanus fruit as a post-planting bio-nematicide was determined on Meloidogyne incognita and growth of tomato in pot trials. Seedlings were inoculated with 1,130 eggs...

Author(s): O. M. Pelinganga, P. W. Mashela, B. Nzanza and M.S. Mphosi

October 2013

Effect of gibberrelic acid on α-amylase activity in heat stressed mung bean (Vigna radiata L.) seedlings

High temperature is a serious threat that can alter the number of biochemical processes in plants, which may lead to reduce crop yield. Gibberellic acid (GA3) is a plant growth hormone, responsible for growth, stress tolerance and regulation of many enzymes like amylase. Amylase is responsible for growth by hydrolyzing starch into maltose. This experiment was carried out to study the effect of GA3 on...

Author(s): Simeen Mansoor and Farzana N. Naqvi    

October 2013

Diversity and frequency of Acacia spp. in three regions in the Kingdom of Saudi Arabia

This study was carried out to assess the diversity and frequency of Acacia spp. and to determine the effect of soil texture on the frequency of Acacia spp. The study sites were selected after field visits to the Al Madinah, Aseer and Al Baha areas. The selection was done on the basis of the natural distribution and density of Acacia spp. Both Aseer and Al Baha are in the semiarid zone, and...

Author(s): Hamad Al-Mefarrej

October 2013

Purification of kappa (k)-carrageenase from locally isolated Cellulosimicrobium cellulans

Partial purification of the crude kappa (k)-carrageenase present in the culture filtrates ofCellulosimicrobium cellulans was carried out by fractional precipitation, using ammonium sulphate, acetone and ethanol individually. The highest recovered protein (37.08%) combined with enzyme activity was obtained with ammonium sulphate. The fraction precipitated by 90% ammonium sulphate was re-purified by anion...

Author(s): Ehab A. Beltagy, Amany S. Youssef, Mohamed A. El-Shenawy and Samy A. El-Assar

October 2013

Shake flask optimization of β-carotene production in Rhodotorula graminis RC04

The present investigation was focused on the effects and interactions of media components on the production of β-carotene by Rhodotorula graminis. Mannitol, potassium nitrate, yeast extract, monopotassium hydrogen phosphate and magnesium sulfate medium components were screened using Plackett Burman Design to identify critical factors. Initial pH of 5.5 of the medium was found to be optimum for β-carotene...

Author(s): Marielou Ferrao and Sandeep Garg

October 2013

In vitro characterization of Saccharomyces cerevisiae HM535662 obtained from an indigenous fermented food “Bhaturu” of Western Himalayas

A predominant yeast designated as SB1 was isolated from Bhaturu, a traditional fermented food of Western Himalayas and was identified as Saccharomyces cerevisiae - HM535662 on the basis of ribosomal gene (partial 18S, complete internal transcribed spacer 1 (ITS1), complete 5.8S, complete ITS2 and partial 28S ribosomal regions) analysis. It was further evaluated for probiotic traits by in...

Author(s): Aditi Sourabh, Sarbjit Singh Kanwar and Om Prakash Sharma

October 2013

In vitro cytotoxicity and induction of apoptosis by multiwalled carbon nanotubes in human peripheral lymphocytes: Correlation with physicochemical properties

Multiwalled carbon nanotubes (MWCNTs) consist of more than 80% of the current nanomaterials’ applications worldwide. Despite their wide application, little information is known concerning their impact on human health. The current study aims to identify the in vitro effects of exposure of the human peripheral blood lymphocytes (PBL) to MWCNTs and the possible associations with their physiochemical...

Author(s): Mohamed F. Elshal, Mohamed Abdel Salam and Jalaluddin A. Khan

October 2013

Aflatoxin status of some commercial dry dog foods in Ibadan, Nigeria

The occurrence of aflatoxins B1, B2, G1 and G2 in commercial dry dog foods in the city of Ibadan, Southwest Nigeria, was investigated. Dry dog food samples from 6 producers were purchased on five different occasions from retail outlets in Ibadan, Nigeria. High performance liquid chromatography was used for separation and quantification of aflatoxin fractions, after consideration of the limits of detection and...

Author(s): Akinrinmade, Joseph Fadeyemi and Akinrinde, Akinleye Stephen

October 2013

Elevated level of serum triglyceride among high risk stress bank employees in Riyadh region of Saudi Arabia

The objective of this study was to estimate lipid profile among high risk stress bank employees’ correlated with heart disorders in Riyadh, Saudi Arabia. A total of 129 patients with high risk stress employees were involved in this study, which were divided into 69 males and 60 females between the age of 25 to 55 years. A total of 80 control...

Author(s): Abbas H Alsaeed

October 2013

In silico design of cyclic peptides as influenza virus, a subtype H1N1 neuraminidase inhibitor

Nowadays, influenza has become a global public health concern because it is responsible for significant morbidity and mortality due to annual epidemics and unpredictable pandemics. There are only limited options to control this respiratory disease. Vaccine treatment is useless for controlling this disease because of the occurrence of mutation in the influenza virus. Influenza virus is also resistant to some antiviral...

Author(s): Usman Sumo Friend Tambunan, Noval Amri and Arli Aditya Parikesit

June 2012

Developing high performance and cost effective real time (PCR) for P-glycoprotein gene expression analysis in rat

Analysis of rare transcripts in tissues or comparative gene expression analysis is one of the main goals of researchers worldwide. Since the development of real time polymerase chain reaction (PCR) for such purpose, there has been raising interest to apply this tool in molecular biology systems, including basic veterinary. We developed an efficient and cost effective real time assay, particularly for rat’s...

Author(s): Sepideh Ghasemian, Hossein Najafzadeh, Masoud Reza Seifi, Amir Jalali and Hamid Galehdari,

June 2012

Identification of HSP90 gene from the Chinese oak silkworm, Antheraea pernyi

The heat shock proteins (Hsp) play an important role in protein folding and protection of cells from stress. To investigate the role of Hsp90 in silk-producing insect Antheraea pernyi (Lepidoptera: Saturniidae), a full-length cDNA encoding Hsp90 from A. pernyiwas cloned, sequenced and characterized. The complete cDNA (2,482 bp) contained a 2,154 bp open reading frame encoding 717 amino acid residues and...

Author(s): Li Chen#, Baojian Zhu#, Guoqing Wei, Lei Yu, Yingying Xu, Chaoliang Liu and Jia Cao

June 2012

Evaluation of seed priming on germination of Gladiolus alatus

Seed priming improves seed performance under environmental conditions. The study was designed to evaluate the effect of different priming treatments on germination behavior of Gladiolus alatus. The experiment was conducted under complete randomized design (CRD) with four replications. Seed priming was done with different concentration of potassium nitrate (KNO3) and hydropriming. All the treatments had significant...

Author(s): Salman Mushtaq, Ishfaq Ahmad Hafiz, Syed Zia Ul Hasan, Muhammad ArifMuhammad Asif Shehzad Rizwan rafique, Misbah Rasheed Muhammad Aliand  Muhammad Shahid Iqbal

June 2012

Effect of nifedipine, imipramine and sertraline on the antidepressant-like actions of furosemide in forced swim (FST) and tail suspension (TST) tests models of depression in mice

The objective of the study was to determine the effect of nifedipine, imipramine and sertraline on the acute and long-term antidepressant-like responses of furosemide in the forced swim (FST) and tail suspension (TST) tests in mice. Groups of mice of six in each group were treated for 30 days with Tween 80, furosemide (10 mg/kg) + nifedipine (5 mg/kg), furosemide (10 mg/kg) + imipramine (10 mg/kg) and furosemide (10...

Author(s): S. E. Oriaifo and E. K. I. Omogbai

June 2012

Cytotoxic effect of nanoparticles synthesized from Salvia officinalis L. and Ricinus communis aqueous extracts against vero cell line and evaluation of their antioxidant activities

The development of safe carriers for the use of plant extracts in industrial and health fields constitute a matter of serious concern. The development of plant derived nanoparticles may help to overcome such barriers. However, the major concern is still the safety of these carriers. The present study describes the synthesis of nanoparticles derived from Salvia officinalis L. and Ricinus communis and...

Author(s): Abid Nabil Ben Salem#, Rouis Zyed#, Lassoued Mohamed Ali, Soualeh Nidhal, Sfar Souad and Aouni Mahjoub

June 2012

Antimicrobial, antioxidant and phytochemical investigation of Balanites aegyptiaca (L.) Del. edible fruit from Sudan

The methanol extract of the fruit (edible mesocarp) of Balanites aegyptiaca (L.) Del., a popular plant from Sudan, widely employed in Sudanese folk medicine was screened for antimicrobial, antioxidant and some active phytochemical compounds. The extract showed the presence of saponin, terpenoids, phenolic compounds and alkaloids. The methanolic extract of the fruits extracted...

Author(s): Emad M. Abdallah, Anis Ben Hsouna, and Khalifa S. Al-Khalifa

June 2012

Purification of kappa (k)-carrageenase from locally isolated Cellulosimicrobium cellulans

Partial purification of the crude kappa (k)-carrageenase present in the culture filtrates ofCellulosimicrobium cellulans was carried out by fractional precipitation, using ammonium sulphate, acetone and ethanol individually. The highest recovered protein (37.08%) combined with enzyme activity was obtained with ammonium sulphate. The fraction precipitated by 90% ammonium sulphate was re-purified by anion...

Author(s): Ehab A. Beltagy, Amany S. Youssef, Mohamed A. El-Shenawy and Samy A. El-Assar

June 2012

Amniotic fluid-derived mesenchymal stem cells as a novel therapeutic approach in the treatment of fulminant hepatic failure in rats

As a potential alternative treatment for terminal liver diseases, amniotic fluid derived mesenchymal stem cells (AFMSCs) have many advantages over other stem cells: avoiding much ethical controversy and decrease in both quantity and differentiation potential with age. However, the therapeutic role of AFMSC for fulminant hepatic failure (FHF) has not yet been clearly elucidated. Therefore, we investigated the reparation...

Author(s): Yu-Bao Zheng, Liang Peng, Ying Yan, Yu-Rong Gu, Geng-Lin Zhang,Zhan-Lian Huang, Pei-Pei Wang, Xiao-Hong Zhang, Cao-Shuang. Lin, Dong-Ying Xie, Bin-Liang Lin, Yu-tian Chong and Zhi-Liang Gao,