African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 29 May 2012; 11(43)

May 2012

Investigation of genetic diversity in flixweed (Descurainia sophia) germplasm from Kerman province using inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers

Flixweed plant bearing the scientific title of ‘Descurainia sophia’ is recognized across the planet as well as in Iran, as an herbal medicine. The present study investigates the genetic diversity of germplasm of flixweed in Kerman Province using and inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers. Flixweed seeds were initially collected from 15 fields in...

Author(s): Kian Aghaabasi and Amin Baghizadeh    

May 2012

Preliminary molecular analysis of the genetic diversity of some Atriplex species present in the Northeast of Algerian

In the arid and semi arid areas, salt bush (Atriplex) represents an important forage resource. The characterization of the genetic diversity of these species is useful for their classification, their conservation and their improvement. In this context, we used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique to test the genotypes of three different species...

Author(s): MÂALEM Souhaïl, KHOUFI Sahari, RAHMOUNE Chaâbane and BENNACEUR M’Barek

May 2012

The study of genetic diversity in some Iranian accessions of Hyoscyamus sp. using amplified fragment length polymorphism (AFLP) and retrotransposon/AFLP markers

Hyoscyamus sp. is well known as a natural source of two main tropan alkaloids including hyoscyamine and scopolamine. The environmental conditions make a very wide diversity of this herb in Iran. This study was conducted to evaluate the genetic diversity within a set of 45 Iranian accessions of Hyoscyamus sp. usingamplified fragment length polymorphism and retro/AFLP markers. 18 primer...

Author(s): Alireza Etminan, Mansoor Omidi, Eslam Majidi Hervan, Mohammad reza Naghavi, Shamsali reza zadeh and Mostafa Pirseyedi

May 2012

Evaluation of the influence of nitrogen fixing, phosphate solubilizing and potash mobilizing biofertilizers on growth, yield, and fatty acid constituents of oil in peanut and sunflower

Three biofertilizers nitrobein, phosphorein, and potash, containing nitrogen fixing, phosphate solubilizing, and potash mobilizing microorganisms, respectively were studied in peanut (Arachis hypogea L.) and sunflower (Helianthus annuusL.). Amendment with each of these biofertilizers enhanced different growth parameters of 30-day-old peanut and sunflower plants. Dry matter in both plants was generally correlated...

Author(s): Hala F. S. Ahmed and Magda M. I. El-Araby

May 2012

Cloning and characterization of an ascorbate peroxidase gene regulated by ethylene and abscisic acid during banana fruit ripening

An ascorbate peroxidase (APX) cDNA, designated MaAPX1, was isolated from banana fruit by suppression subtractive hybridization (SSH). MaAPX1 shares an extensive sequence identity (79 to 83%) with other plant APX homologues. Southern blot analysis revealed only two copies of the APX gene in the banana genome. Reverse-transcriptase PCR analysis of MaAPX1 expression confirmed its expression in the...

Author(s): Zhuo Wang, Zhi-Qiang Jin, Jia-Shui Wang, Mei-Ying Li, Cai-Hong Jia, Ju-Hua Liu, Jian-Bin Zhang and Bi-Yu Xu    

May 2012

Regeneration from embryogenic callus and suspension cultures of the wild medicinal plant Cymbopogon schoenanthus

Somatic embryogenesis and plant regeneration from both callus and suspension cultures of the wild medicinal plant Cymbopogon schoenanthus subsp.proximus has been achieved. The species is rare and confined in its distribution to Africa. A range (0.5 to 8 mg/l) of 2,4-dichlorophenoxyacetic acid (2,4-D) for the induction of embryogenic callus from seed cultures were used. Results show that 1.0 and 4.0 mg/l...

Author(s): Ahmed A. El-Bakry and Asmaa M. Abdel-Salam    

May 2012

Influence of sulfur on cadmium (Cd) stress tolerance in Triticum aestivum L.

A pot experiment was conducted to investigate the influence of sulfur on cadmium (Cd) stress tolerance in Triticum aestivum L. (cv. Samma). The treatments were given as follows: 0 mM S + 0 mM Cd (control), 1 mM S + 0 mM Cd (S1), 5 mM S + 0 mM Cd (S2), 10 mM S + 0 mM Cd (S3), 0 mM S + 1 mM Cd (Cd), 1 mM S + 1 mM Cd (S1+Cd), 5 mM S + 1 mM Cd (S2+Cd),) and 10 mM S + 1 mM Cd (S3+Cd). Plants fed with Cd showed...

Author(s): Abdel-Rhman Z. Gaafar, Ahmad A. Ghdan, Manzer H. Siddiqui, Mohamed H. Al-Whaibi, Mohammed O. Basalah, Hayssam M. Ali and Ahmed M. Sakran    

May 2012

Waste to wealth: Production of oxytetracycline using streptomyces species from household kitchen wastes of agricultural produce

The production of oxytetracycline by Streptomyces speibonae OXS1 in solid-state fermentation from cocoyam peels (household kitchen wastes of agricultural produce) was investigated. The proximate analyses of peels of the two cocoyam species showed that Colocasia esculenta had higher protein (1.39%) and fibre (15.70%) contents than Xanthosoma esculenta with protein and fibre contents of 0.91...

Author(s): Tobias I. Ndubuisi Ezejiofor, Carissa I. Duru, Agnes E. Asagbra, Anthonet N. Ezejiofor, Orish E. Orisakwe, Johnson O. Afonne and Ejeatuluchukwu Obi    

May 2012

Biochemical characterization of thermostable cellulase enzyme from mesophilic strains of actinomycete

A few mesophilic strains of actinomycete were used for detection, extraction and characterization of cellulase enzymes. These strains responded to produce all the three components of cellulase complex (endoglucanase, exoglucanase and β-glucosidase) in balanced quantities. Cellulase activity was determined on solid medium supplemented with 1% carboxy methyl cellulose (CMC). Production of cellulase was detected by...

Author(s): Khwaja Salahuddin, Ram Prasad, Suresh H. Gor, Manish D. Visavadia, Virendra K. Soni and M. Dilshad Hussain    

May 2012

Comparison of nutritional values of brown and white beans in Jos North Local Government markets

Cowpea is an important bean which play significant role in the diets of Africans. It serves as a major source of protein in the absence of sufficient animal protein for the population. Two varieties (white and brown) of cowpeas (Vigna unguiculata) were analyzed for their proximate and elemental contents. These varieties belong to the same species in the family leguminosae. The brown and white seeds were found to be...

Author(s): Alayande, L. B., Mustapha, K. B., Dabak, J. D. and Ubom, G. A.    

May 2012

The effectiveness of polyethylene glycol (PEG) and polyvinyl polypyrrolidone (PVPP) on removal of tannins from leaf extracts of selected medicinal plants in Limpopo Province

Five selected plant species (Balanites maughamii, Breonadia salicina,Dombeya rotundifolia, Hyperacanthus amoenus and Piliostigma thonningii)were extracted using 70 and 100% acetone and the presence of tannins were determined using radial diffusion assay. Our comparative assessment indicated that plant extracted with 100% acetone showed the highest levels of tannins as compared to 70%...

Author(s): Mahlo SM, and Hasani Chauke    

May 2012

Preliminary study on the effect of castration and testosterone replacement on testosterone level in the New Zealand male rabbit

To study the effect of castration and testosterone replacement on the testosterone level of the New Zealand rabbit, 16 apparently healthy adult male rabbits were used. The animals were divided into four groups with each group having four rabbits. The first group served as the control group. The rabbits in the second group were unilaterally castrated, while those in the third group were bilaterally castrated. The rabbits...

Author(s): A. A. Sanni, R. O. A. Arowolo and F. O. Olayemi    

May 2012

Antinociceptive potential of Parkia platycephala Benth. in streptozotocin-induced diabetic rats

This study aimed to investigate the possible antinociceptive action of the ethanolic extract (E.EtOH) and ethyl acetate fraction (F.AcOEt) from leaves ofParkia platycephala in streptozotocin (STZ)-induced diabetic rats. In chronic experiment, STZ-rats were daily treated with E.EtOH (150 or 300 mg/kg, p.o) for three weeks. The mechanical nociceptive threshold (MNT) was...

Author(s): Vivianne R. Amorim, Herton L. A. S. Filho, Roosevelt D. S. Bezerra, Mariana H. Chaves, Fernanda R. C. Almeida and Salete M. R. C. Brito    

May 2012

Study on stomach physiological functions by electroacupuncturing at zusanli points under effects of atropine and cimetidine

To explore the effect and mechanism on the physiological functions of stomach by electroacupunctue at zusanli (STOMACH-36), the changes of the gastric electrical frequency and amplitude, and the flux of gastric juice secretion were observed with modern apparatus, when electroacupuncture at zusanli was administered on rabbits, before and after injecting atropine and cimetidine. The experimental results show that the...

Author(s): Yong Peng, Man Sun, Zhe Liu, Jianxin Wang, Yingjie Liu, Yanxia Zhang and Bin Jiang