African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 29 November 2012; 11(96)

November 2012

Microbial degradation of toluene

Zephirus environment is polluted by the toluene from profuse range industries. In spite of positive potential application, toluene results in many mishaps especially health hazards; hence amputation of toluene is crucial for human welfare as well as environmental issues. This review deals with destruction of toluene using microbial degradation. The overall aerobic biodegradation of toluene into carbon dioxide, water and...

Author(s): M. Gopinathand R. Dhanasekar

November 2012

Characterization of some enzymatic properties of recombinant a-glucosidase III from the Thai honeybee, Apis cerana indica Fabricus

Recombinant a-glucosidase III (rHBGase III) from Apis cerana indica Fabricus (rAciHBGase III) was expressed in the yeast Pichia pastoris GS115, enriched and characterized. The full length cDNA of AciHbgase III (~1.8 kb) was amplified by RT-PCR, cloned into the pPICZaA expression vector and used to transform P. pastorisGS115. The maximum secreted expression level of rAciHBGase III...

Author(s): Jirattikarn Kaewmuangmoon, Mikio Yoshiyama, Kiyoshi Kimura, Masayuki Okuyama, Haruhide Mori, Atsuo Kimura and Chanpen Chanchao

November 2012

Genetic diversity and DNA fingerprint study in tomato (Solanum lycopersicum L) cultivars grown in Egypt using simple sequence repeats (SSR) markers

A collection of ten cultivars of tomato grown in Egypt were screened with 20 simple sequence repeat (SSR) primers in order to determine genetic identities, genetic diversity and genetic relationships among these cultivars. On an average, 38 alleles were amplified using SSR primers with scorable fragment sizes ranging from approximately 75 to 275 bp. 23 alleles were polymorphic thus revealing 60.5% of polymorphism. The...

Author(s): Mohamed A. M. El-Awady, Adel A.E. El-Tarras, and Mohamed M. Hassan,

November 2012

Antioxidative and physiological studies on Colocasia esculentum in response to arsenic stress

The current study was undertaken to determine the effects of arsenic on Colocasia esculentum. Rhizomes were grown in pots containing 2.5 kg of garden soil with increasing concentration of arsenic. Arsenic accumulation was more in shoots compared to roots at higher concentrations. High arsenic concentration caused reduction in plant growth along with induction of few antioxidants. C. esculentum has a...

Author(s): Mandakini J. Patel, Pritesh Parmar, Bhaumik Dave and R. B. Subramanian

November 2012

Biological control of Meloidogyne incognita by Trichoderma harzianum and Serratia marcescens and their related enzymatic changes in tomato roots

Biological control against the root-knot nematode, Meloidogyne incognita was proven to occur in tomato, Solanum lycopersicom, soil-drenched with different isolates ofTrichoderma harzianum and a commercial suspension of Serratia marcescens(Nemaless). The potential of such biocontrol agents to trigger plant defense response was discussed. Nematode reproduction in the presence of such...

Author(s): Mahfouz M. M. Abd-Elgawad and Sanaa S. A. Kabeil

November 2012

Accumulation pattern of total nonstructural carbohydrate in strawberry runner plants and its influence on plant growth and fruit production

The pattern of total nonstructural carbohydrate (TNC) accumulation in strawberry (Fragaria ananassa Duch.) nursery runner plants, cv. ‘Camarosa’, was determined for three growing seasons. Plant growth and fruit production patterns were also evaluated. The experiments were carried out on plants propagated in high latitude (41°50' N) and high elevation (1292 m) nurseries in Siskiyou County,...

Author(s): Daniel S. Kirschbaum, Kirk D. Larson, Steve A. Weinbaum and Theodore M. DeJong

November 2012

Analysis of phthalate esters contamination in drinking water samples

Phthalate esters are widely used as plasticizer. They can migrate from plastic materials to the environment. Exposure to some of these compounds may increase the risk of getting cancer. Phthalate esters: dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) phthalate (DEHP) were extracted simultaneously using a solid-phase extraction and compared with liquid-liquid extraction...

Author(s): W. Kanchanamayoon, P. Prapatpong, S. Chumwangwapee and S. Chaithongrat

November 2012

Manipulating nutrient composition of microalgal growth media to improve biomass yield and lipid content of Micractinium pusillum

Biodiesel production from microalgae depends on the algal biomass and lipid content. Both biomass production and lipid accumulation are limited by several factors in which nutrients play a key role. We investigated the influences of micronutrients on biomass, and lipid content of Micractinium pusillum GU732425 cultivated in bold basal media (BBM). The average dry biomass of microalgal strain in control medium...

Author(s): Reda A.I. Abou-Shanab, Sapireddy V. Raghavulu, Nagah M.A. Hassanin, Seongheon Kim, Yong Je Kim, Sang Un Oh, You-Kwan Oh, and Byong-Hun Jeon

November 2012

Histological changes and biochemical parameters in the hepatopancreas of terrestrial gastropod Helix aspersa as biomarkers of neonicotinoid insecticide exposure

  In this study, adult snails, Helix aspersa were used to estimate the effect of aneonicotinoid insecticide (thiametoxam) on biochemical parameters and histological changes in the hepatopancreas of this gastropod after a treatment of six weeks. During this period, snails were exposed by ingestion and contact to fresh lettuce leaves which were soaked with an insecticide solution. The thiametoxam test...

Author(s): Smina Ait Hamlet, Samira Bensoltane, Mohamed Djekoun, Fatiha Yassi and Houria Berrebbah

November 2012

Antimicrobial resistance and plasmid profiles of Aeromonas hydrophila isolated from River Njoro, Kenya

The purpose of this study was to investigate the presence of Aeromonas hydrophila at commonly used water collection points on the River Njoro and to determine the in-vitro antimicrobial susceptibility and plasmid profiles of isolates. In total, 126 samples were collected and 36.5% of them were positive for A. hydrophila. The A. hydrophilawere recovered on membrane filters,...

Author(s): Njeru S. Ngoci , Kiruki Silas , Limo Moses, Mbala M. Jessica , Eliud Nyaga Mwaniki Njagi, Paul O. Okemo  and Lawless Nathan

November 2012

Genetic differentiation and gene flow between the Tunisian ovine breeds Barbarine and Western thin tail using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis

Sheep is an important livestock species of Tunisia. They contribute greatly to the food safety of the country and in the livelihood of a large number of small and marginal farmers and landless labourers engaged in sheep rearing. In this study, random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis was used to assess the genetic difference and gene flow among two Tunisian sheep breeds (the...

Author(s): Haifa El Hentati, Mohamed Ben Hamouda and Ali Chriki