African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 30 January 2013; 12(5)

January 2013

Fruit maturation and in vitro germination of macaw palm embryos

  Acrocomia aculeata (macaw palm) is oil producing palm tree with significant agro-industrial potential. Seed dormancy in palm species may be due to embryo immaturity, which could result from delayed embryogenesis. We evaluated the correspondence between the visual characteristics of maturing fruits and their physiological aspects and the in vitro germination capacity of the embryos. 11 fruit...

Author(s):   Priscila Oliveira Silva, Leonardo Monteiro Ribeiro, Maria Olívia Mercadante Simões, Paulo Sérgio Nascimento Lopes, Teddy Marques Farias and Queila Souza Garcia        

January 2013

Ecological assessment of Great Lota Lake (Turkey) on the base of diatom communities

  The diatoms are very important component for aquatic ecosystems. Turkey has a rich lake potential and  many of the lakes have high level of endemism. For this reason, the Great Lota Lake was investigated  between October 2000 and October 2001 in sampling periods of approximately per 15 days from one  station. Totally, 104 diatom taxa were identified and used for ecological analysis...

Author(s):   Rıdvan Erdal Sivaci,  Sophia Barinova,  Cüneyt Nadir Solak  and Kadir Çobanoglu

January 2013

Production and characterization of cellulolytic activities produced by Trichoderma longibrachiatum (GHL)

  The indigenous cellulolytic fungus Trichoderma longibrachiatum (GHL) isolated from soil near an Algerian hot spring was used for the production of cellulases by submerged fermentation on Mandels medium with cellulose Avicel (1%) as the sole carbon source. Endoglucanase and filter paper activities of the wild-type strain ofTrichoderma were compared to the hypercellulolytic...

Author(s):   Hind Leghlimi, Zahia Meraihi, Hayet Boukhalfa-Lezzar, Estelle Copinetand Francis Duchiron      

January 2013

Antibacterial activity of root and leaf extracts of Jatropha zeyheri Sond (Euphorbiaceae)

  Resistance of human pathogenic bacterial strains results in selective pressure against known antibiotics. Jatropha zeyheri is used by traditional medicine practitioners in the treatment of sexually transmitted and urinary tract infections. Acetone, methanol and ethyl acetate extracts of both leaves and roots of Jatropha zeyheri collected from Capricorn District, Limpopo Province, South...

Author(s):   Mongalo NI, Opoku AR and Zobolo AM        

January 2013

Enhanced production of poly glutamic acid by Bacillus sp. SW1-2 using statistical experimental design

  Bacillus sp. SW1-2 producing poly glutamic acid (PGA), locally isolated from Eastern province in Saudi Arabia, was characterized and identified based on 16S rRNA gene sequencing. Phylogenetic analysis revealed its closeness to Bacillus megaterium. The homopolymer consists mainly of glutamic as indicated in the analysis of amino acid. Preliminary optimization of PGA production, through a series of...

Author(s):   Mahmoud M. Berekaa, and Mohamed S. Al-Otaibi        

January 2013

Stability profile of flavour-active ester compounds in ale and lager beer during storage

  Currently, one of the main quality problems of beer is the change of its chemical composition during storage, which alters its sensory properties. In this study, ale and lager beers were produced and aged for three months at two storage temperatures. Concentration of volatile ester compounds (VECs) in the beers was regularly monitored by gas chromatographic analysis of the headspace samples to establish...

Author(s):   Lettisha Hiralal, Balakrishna Pillay and Ademola O. Olaniran        

January 2013

Acute toxicity of the chloroacetanilide herbicide butachlor and its effects on the behavior of the freshwater fish Tilapia zillii

  Acute toxicity of butachlor, a chloroacetanilide herbicide (2-chloro-N-[2, 6-diethylphenyl] acetamide) to Tilapia zillii, was studied in a semi static bioassay. The 24, 48, 72 and 96 h LC50 values (with 95% confidence limits) estimated by probit analysis were 3.13 (2.88 to 4.61), 1.93 (0.63 to 4.41), 1.27 (0.59 to 1.92) and 1.25 (0.60 to1.85) mgl-1, respectively. There were significant differences...

Author(s):   Christopher Didigwu Nwani, Udu Ibiam Ama, Florence Okoh, Uche Obasi Oji, Rebecca Chima Ogbonyealu, Arua Agha, Ibiam and Onyinyechi Udu-Ibiam    

January 2013

Isolation, characterization and molecular weight determination of collagen from marine sponge Spirastrella inconstans (Dendy)

  Collagen is a major structural protein of connective tissues. It can be used as a prosthetic biomaterial applicable to artificial skin, tendon ligaments and development collagen implants. In the present study, an attempt was made to isolate and characterize collagen from the marine sponge, Spirastrella inconstans. The total protein content of sponge collagen was relatively high (32%). While...

Author(s):   S. Sudharsan, P. Seedevi, R. Saravanan, P. Ramasamy, S. Vasanth Kumar, S. Vairamani, A. Srinivasan and A. Shanmugam    

January 2013

New purification process of fungal immunomodulatory protein, FIP-fve from Flammulina velutipes via filtration and column chromatography

  Microfiltration, ultrafiltration, cation exchange and size-exclusion chromatography were used to isolate FIP-fve from Flammulina velutipes fruiting body in the pilot scale. At the sample preparing stage, the homogenate of mushroom is usually hard to be resolved hence microfiltration was used to clarify the homogenate of F. velutipes efficiently. Next ultrafiltration is applied to...

Author(s):   Yuequn Cao#, Chongyang Liang#, Yandong Zhang, Jianlin Lin and Fei Sun        

January 2013

Haematological indices of Channa punctatus as an indicator of heavy metal pollution in waste water aquaculture pond, Panethi, India

  Investigations on the concentration of heavy metals (Cu, Ni, Fe, Co, Mn, Cr and Zn) in water and their effects on haematological parameters were observed in Channa punctatus. The results reveal that heavy metals available in water were in the order Fe > Mn > Zn > Co > Ni > Cu = Cr. In the present study, the haematological data of C. punctatus revealed that significant...

Author(s):   Mehjbeen Javed and Nazura Usmani