African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 31 May 2012; 11(44)

May 2012

Arabidopsis rad23-4 gene is required for pollen development under UV-B light

Nucleotide excision repair (NER) is a highly conserved DNA repair pathway for correcting DNA lesions that cause distortion of the double helical structure. The protein heterodimer Rad23 is involved in recognition and binding to such lesions. Here, we showed that rad23-4 (AT5g38470) was expressed in the roots, mature leaves, floral buds and developing siliques. The collapsed pollen grains were observed...

Author(s): Xiushan Li , Xinhong Guo, Lijian Zhao, Jihong Zhang, Dongying Tang, Xiaoying Zhao, Xuanming Liu    

May 2012

A new caerin-like antibacterial peptide from the venom gland of the Iranian scorpion Mesobuthus eupeus: cDNA amplification and sequence analysis

Scorpion venom consists of different types of peptides and proteins which are encoded by individual genes. A full length cDNA consisting of 238 base pair nucleotides and encoding 74 amino acids peptide was isolated from the venom gland of the Iranian scorpion Mesobuthus eupeus (Buthidae family). This peptide named M. eupeus caerin-like antimicrobial peptide (Me-CLAP) belonging to the group of...

Author(s): Masoumeh Baradaran, Amir Jalali, Abbas Jolodar and Sepideh Ghasemian    

May 2012

Enhanced in vitro multiple shoot induction in elite Pakistani guava cultivars for efficient clonal plant multiplication

Elite guava (Psidium guajava L.) strains of cv. Safeda were explored in vitro for multiple shoot induction. Shoot induction was enhanced up to 83% with 3.5 to 4.25 shoots per single node cutting and shoot tip explants, respectively, using higher levels of benzyl amino purine (BAP) in Murashige and Skoog (MS) medium. Enhancing sucrose supplement to 45 gl-1 improved plant growth and...

Author(s): Muhammad Usman, Madiha Butt and Bilquees Fatima      

May 2012

Morphological and anatomical response of Acacia ehrenbergiana Hayne and Acacia tortilis (Forssk) Haynes subspp. raddiana seedlings to induced water stress

The response of Acacia ehrenbergiana Hayne and Acacia tortilis (Forssk) Haynes subspp. raddiana seedlings to 100, 50 and 25%  field capacity (FC)watering regimes was studied to determine their morphological and anatomical behaviour. Both species responded morphologically as well as anatomically to water stress. Water stress caused significant (P=0.05) decrease in relative water...

Author(s): Hashim Ali El Atta, Ibrahim Mohamed Aref, Abdalla Ismail Ahmed and Pervez Rasheed Khan    

May 2012

Detection of Hevea brasilensis clones yield potential and susceptibility to tapping panel dryness in Côte d’Ivoire using the 32 and 35 KDa lutoidic proteins

This study was carried out to identify protein markers with yield potential and susceptibility to tapping panel dryness (TPD). To achieve this goal, 11 clones, stimulated and non stimulated, yield and susceptibility to TPD were compared. Their lutoid fraction polypeptides were analysed using one and two-dimensional electrophoresis. Susceptibility to TPD appeared as a clonal trait which is not related...

Author(s): Elabo Agnyman Angeline Eliathe, Koffi kouablan Edmond, Okoma koffi Mathurin, Lidah Yéhili Justin, NGuetta Assanvo Simond Pierre, Dian Kouadioand Sangaré Abdourahamane    

May 2012

Effect of hormone treatments on deformed fruit development in pear

Fruit shape is one of the most important limiting factors in the quality of pear fruits. The effects of applied plant growth regulators on fruit shape have been determined in pear. The application of naphthalene acetic acid (NAA, 20 mg/L),indole-3-butyric acid (IBA, 30 mg/L), 6-benzalyamino acid (6-BA, 30 mg/L), cleaner production promotion unit (CPPU, 10 mg/L), gibberrelin (GA4+7, 3%)...

Author(s): Xiaoming Chen, Jianping Bao, Yiting Chen, Ting Chen, Changhe Zhang, and Xinzhong Huang    

May 2012

Characterization of p-nitrophenol degrading actinomycetes from Hundung limestone deposits in Manipur, India

p-Nitrophenol (PNP), a major nitroaromatic xenobiotic is released into the environment as a result of its widespread use and as a breakdown product of organophosphate (OP) agricultural pesticides such as parathion and methyl parathion. It is highly toxic to soil microflora and other non-target organisms. Of several Microbial Biotechnology Research Laboratory (MBRL) strains isolated from limestone deposits in Ukhrul,...

Author(s): Debananda S. Ningthoujam, Suchitra Sanasam and Anjuna Mutum    

May 2012

Application of fluorescence in situ hybridization (FISH) to the analysis of sulfate reducing bacterial community in an oily bench scale reactor

Advances in the field of genomics and meta-genomics have led to rapid and accurate strategies for the monitoring of microbial biodiversity and have revealed its potential for biotechnological applications. In this study, fluorescent in situ hybridization (FISH) as a culture-independent molecular approach using specific CY3-labelled oligonucleotide probes was used to study the dynamics of the sulfate reduction...

Author(s): Abd El-Latif Hesham,  and Saad A. Alamri    

May 2012

Biochemical properties and microbial analysis of honey from North-Western regions of Iran: Seasonal effects on physicochemical properties of honey

Author(s): Razzaqh Mahmoudi, Payman Zare, Hossein Tajik, Sina Shadfar and Farzad Nyiazpour    

May 2012

Effects of debranning and ultra-fine grinding on the quality of flour and cookies from blue wheat

The effects of debranning levels of 4 and 8% and ultra-fine grinding on physico-chemical, rheological, and pasting properties of wheat flour obtained from blue wheat, as well as cook quality, were investigated. The process of debranning decreased ash (from1.89 to 1.62%) and wet gluten content (from 44.68 to 38.81%), but increased the gluten index of wheat flours (from 12.95 to 17.82). Debranning had positive effects on...

Author(s): Feifei Luo, Xiansheng Zhang, Hanxue Hou, Haizhou Dong, Jinli Zhang and Chenqi Li    

May 2012

A chitosanase purified from the snail of Achatina fulica

A chitosanase from the snail of Achatina fulica was purified 18.27-fold with 0.68% recovery of protein and 12.53% recovery of enzymatic activity by phenyl-sepharose column chromatography, diethylaminoethanol (DEAE)-sepharose column chromatography and Sephacryl S-300 gel filtration. The molecular mass of chitosanase was 72 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)...

Author(s): Ming-Zhong Sun#, Yun Zhu#, Chunmei Guo, Li Su and Yi Xin    

May 2012

Antibacterial effects of zinc oxide nanoparticles on Escherichia coli K88

This study was conducted to evaluate the antibacterial effects of zinc oxide nanoparticles in vitro. Escherichia coli K88 was chosen as an indicator of pathogenic bacteria, because it could cause diarrhea in both children and in early-weaned piglets. In this study, the characterization of the nanoparticles was examined. Antibacterial activities against E. coli K88 were evaluated by...

Author(s): Chao Wang, Lian-Long Liu, Ai-Ting Zhang, Peng Xie, Jian-Jun Lu and Xiao-Ting Zou    

May 2012

Desiccation-induced changes in viability, lipid peroxidation and antioxidant enzyme activity in Mimusops elengi seeds

Intermediate seeds of Mimusopsis elengi showed obvious membrane lipid peroxidation during desiccation. When the moisture content (MC) decreased from initial 41.8 to 6.1%, seed viability significantly decreased from 100 to 23%, consorted with activity changes of a few anti-oxidative enzymes. The activities of superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR)...

Author(s): Anjun Tang    

May 2012

Resveratrol protects against hazardous effects of 2-bromopropane on maturation of mouse oocytes, fertilization and fetal development

2-Bromopropane (2-BP) is regularly used as an alternative to ozone-depleting cleaning solvents. Previously, we reported the cytotoxic effects of 2-BP on oocyte maturation and subsequent pre- and post-implantation development, both in vitroand in vivo. In the current study, we further demonstrate that these hazardous effects are suppressed by resveratrol, a grape-derived phytoalexin with known antioxidant and...

Author(s): I-Meng Huang and Wen-Hsiung Chan    

May 2012

The seasonal distribution of airborne fungi in two hospitals in Istanbul

Changes in fungal concentrations depend on seasonal and environmental conditions. The aim of this study was to determine the seasonal distributions of the fungal flora in Dr. Siyami Ersek Chest, Heart and Vascular Center Education and Research Hospital (SEH) and Kartal Yavuz Selim State Hospital (KYSH) in Ä°stanbul. Samples were collected seasonally from different stations (microbiology laboratory, toilet,...

Author(s): Iskender KARALTI and Günay ÇOLAKOÄžLU    

May 2012

Effect of similar feeding regime on growth and body composition of Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) under mono and polyculture

Growth performance and body composition of yearling Indian major carps (Catla catla, Cirrhinus mrigala and Labeo rohita) was evaluated in semi-intensive (mono and polyculture) systems for 90 days. Prior to stocking, all ponds were fertilized with organic and inorganic manures. This application was repeated every two weeks throughout the study period. Supplementary feed containing 35% protein was applied daily...

Author(s): Noor Khan, Muhammad Ashraf, Naureen Aziz Qureshi, Pallab Kumer Sarker, Grant William Vandenberg and Fayyaz Rasool    

May 2012

Genetic diversity in Indian sub-continental landrace cultivars of the genus Triticum L.

Narrowing genetic diversity is a limiting factor in wheat breeding. Popularity of semi dwarf cultivars, developed after green revolution, has resulted in genetic erosion as they replaced indigenous cultivars derived from landraces. These old cultivars have a wealth of useful genes that can be incorporated in the modern cultivars to improve their tolerance level against biotic and abiotic stresses. Genetic analysis of...

Author(s): Azeem Akram, Azeem Iqbal Khan, Faisal Saeed Awan, Aziz ur Rehman, Faiz Ahmad, Amjad Malik and Sajjad Rao