African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 3 January 2012; 11(1)

January 2012

A single nucleotide polymorphism (SNP) assay for population stratification test between eastern Asians in association studies

  Population subdivision or recent admixture of populations can lead to spurious associations between the certain phenotypes and unlinked candidate loci in case-control and cohort studies of association. False positive associations due to population stratification can be controlled by genotyping a few dozen unlinked genetic markers. Lots of researches have reported SNP markers with high ethnic differentiation...

Author(s):   Tianbo Jin#, Gang Li#, Dong Lin, Hongjuan Liang and Qing Wang  

January 2012

Molecular characterization of early blight disease resistant and susceptible potato cultivars using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers

  Potato early blight disease caused by Alternaria solani is one of the major factors limiting potato production worldwide. Developing highly resistant cultivars is the most effective way to control the disease. In this study, 20 random amplified polymorphic DNA (RAPD) and six simple sequence repeats (SSR) primers were applied to assess the genetic diversity and to identify molecular markers...

Author(s):   Mahmoud H. El_Komy, Amgad A. Saleh, and Younes Y. Molan  

January 2012

Isolation, sequence identification and tissue expression profile of a novel ribokinase gene (RBKS) from Chinese Banna mini-pig inbred line (BMI)

  The complete expressed sequence tag (CDS) sequence of Banna mini-pig inbred line (BMI) ribokinase gene (RBKS) was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) based on the conserved sequence information of the cattle or other mammals and known highly homologous swine ESTs. This novel gene was then deposited into NCBI database and assigned to accession number JF944892....

Author(s):   Jinlong Huo,, Pei Wang, Yongwang Miao, Hailong Huo, Lixian Liu, Yangzhi Zeng, and Heng Xiao

January 2012

Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis

  ABC transporters are membrane proteins that use the energy released from the hydrolysis of ATP to drive the transport of compounds across biological membranes. In some plants, pathogenic fungi ABC transporters play a role as virulence factors by mediating the export of plant defense compounds or fungal virulence factors.Mycosphaerella fijiensis, the causal agent of black Sigatoka disease in banana, is...

Author(s):   Y. Couoh-Uicab, I. Islas-Flores, N. Kantún-Moreno, L.-H. Zwiers, M. Tzec-Simá, S. Peraza-Echeverría, L. Brito-Argáez, L. Peraza-Echeverría, R.Grijalva-Arango, A. James, C. Rodríguez-García, and B. Canto-Canché  

January 2012

Epithelial-mesenchymal transition is associated with increased invasiveness of side population cells from hepatoma SMMC-7721 cells

  To study the in vitro invasiveness of side population (SP), cells purified from hepatoma SMMC-7721 cells and the expressions of epithelial-mesenchymal transition (EMT) markers of SP cells and non-SP (NSP) cells, fluorescence-activated cell sorting (FACS) was used to sort SP cells and NSP cells from SMMC-7721 cells. The in vitro invasiveness of both subpopulations was tested by...

Author(s):   Tao Huang, Dongwei Gong, Quanli Gao, Xuhua Zhang, Xiaodong Lv, Hongbo Ma, Baishun Wan and Ziming Dong  

January 2012

Identification and characterization of the Bcl-2-associated athanogene (BAG) protein family in rice

  The Bcl-2-associated athanogene (BAG) proteins are involved in the regulation of Hsp70/HSC70 in animals. There are six BAG genes in human that encode nine isoforms with different subcellular locations. Arabidopsis thaliana is reported to contain seven BAG proteins. We searched BAG proteins in Oryza sativa using profile-sequence (Pfam) and profile-profile (FFAS) algorithms and found six...

Author(s):   Rashid Mehmood Rana, , Shinan Dong, Zulfiqar Ali, Azeem Iqbal Khan and Hong Sheng Zhang

January 2012

Genetic diversity of Sclerocarya birrea subspecies birrea populations in Burkina Faso detected by RAPDs

  Sclerocarya birrea, multipurpose plant is characteristic of the Sahel-Sudaniansavanna and is widespread in West Africa. Although this species has a high socio-economic importance, its genetic organization was not well characterized in Burkina Faso. In this study, the intra and interpopulation genetic...

Author(s):   Pauline Bationo/Kando, Cyrille Bisseye, Romaric K. Nanema, Ernest R. Traore, Henri Ye, Boukary O. Diallo, Tegwinde R. Compaore, Jacques Simpore and Jean-Didier Zongo  

January 2012

A taxonomic study of the genus Fibigia Medik. (Brassicaceae)

  In this study, Purpureae A.Duran and Ö.Çetin sect. nov. is described under the genus Fibigia Medik. The taxa of the genus were revised under the sections Fibigiaand Purpureae. Fibigia clypeata (L.) Medik was classified as F. clypeata (L.) Medik subsp. clypeata and subsp. anatolica A.Duran & TuÅŸtaÅŸ subsp. nov.,...

Author(s):   Özlem Çetin, Ahmet Duran, Esra Martin and Süleyman TuÅŸtaÅŸ    

January 2012

Influence of AgNO3 on somatic embryo induction and development in Manchurian ash (Fraxinus mandshurica Rupr.)

  In this present study, we explored the effects of silver nitrate (AgNO3) on somatic embryo induction and the development from immature zygotic embryos in Manchurian ash (Fraxinus mandshurica Rupr.). AgNO3 played a minor role on in vitro embryo induction frequency and in the number of somatic embryos per explant. However, 1 mg L–1 AgNO3 enhanced synchronization and...

Author(s):       Dong-mei Kong, , Hai-long Shen and Nan Li          

January 2012

The micro-minerals composition in serum of rabbits (Oryctolagus cuniculus) infected with Trypanosoma congolense

  Sixteen (16) rabbits aged between 6 to 12 months were infected with fresh stock ofTrypanosoma congolense (Gboko strain) intravenously at the rate of 1.0 × 106/ml. Animals were classified into two groups; groups A were infected, while group B served as uninfected controls. Samples from the infected and the uninfected controls showed a  significant increase in the levels of sodium (Na+), calcium...

Author(s):   S. A. Ameen, E. A. Okewole, O. S.Adedeji, K. A. Ogundipe and A.  A. Okanlawon  

January 2012

Effects of nitrogen levels on growth, yield and nitrogen uptake of fiber-rich cultivar, Goami 2

  To investigate the effect of nitrogen (N) application level on fiber-rich cultivar, Goami 2, an experiment was carried out on a paddy soil at the rice experimental farm of the National Institute of Crop Science (NICS), Rural Development Administration (RDA) in Suwon, Korea. Rice growth, yield and its components for Goami 2 were investigated under different N application levels (0, 50, 70, 90, 110, 130 and 150...

Author(s):   Weon Tai Jeon

January 2012

Reproducible and expedient rice regeneration system using in vitro grown plants

  Inevitable prerequisite for expedient regeneration in rice is the selection of totipotent explant and developing an apposite combination of growth hormones. Here, we reported a reproducible regeneration protocol in which basal segments of the stem of the in vitro grown rice plants were used as ex-plant. Using the protocol, callus was developed from the exposed cells of root segments and cortical...

Author(s):   Faiz Ahmad Joyia, and Muhammad Sarwar Khan,  

January 2012

Modeling and analysis of different control strategies for fogging system in a subtropical greenhouse

  A dynamic environment model was established on the effects of greenhouse cooling on different control strategies in a fogging system, which simulations were then conducted by Matlab. Simulation results showed that by applying a suitable set of control strategy, the indoor temperature dropped and was less than the outdoor temperature of 34.4°C The temperature reduction degree changed within limits and...

Author(s):   S. Lee, Y. Huang, C. Chen and C. Chang  

January 2012

Phenylalanine ammonia-lyase (PAL) gene activity in response to proline and tyrosine in rosemary callus culture

  Phenylalanine ammonia-lyase (PAL) catalyzes the biosynthesis of rosmarinic acid (RA), tyrosine and phenylalanine are the precursors of RA, while proline drives metabolite precursors toward Shikimate and phenylpropanoid pathway ending with the production of RA. The aim of this study was to investigate the PAL gene activity in the callus of rosemary (Rosmarinus officinalis) due to exogenous application of...

Author(s):   Hany M. El-Naggar

January 2012

Effect of water deficit stress on proline contents, soluble sugars, chlorophyll and grain yield of sunflower (Helianthus annuus L.) hybrids

  The objective of the present work was to determine the mechanisms of tolerance of four sunflower hybrids; H1 = Azargol, H2 = Alstar, H3 = Hysun 33 and H4 = Hysun 25 to water stress under three different levels of irrigation regimes; WD1 = irrigation after 50 mm (normal irrigation), WD2 = 100 mm (mild stress) and WD3 = 150 mm (intense stress) cumulative evaporation from...

Author(s):   Hussein Oraki,  Fataneh Parhizkar khajani and Majid Aghaalikhana  

January 2012

Effect of a three-month football training program on trace element metabolism of boys in the eight to twelve age group

  This study was conducted to examine the effects of a three-month football training program on some trace elements in the serum in male kids aged between eight and twelve years. The study registered eight boys whose mean age was 10.25 ± 0.75 years, mean height was 138.63 ± 3.28 cm and mean weight was 32.13 ± 2.72 kg. The subjects were regularly trained in the accompaniment of coaches for...

Author(s):   Ersan Kara

January 2012

Rapid DNA extraction of bacterial genome using laundry detergents and assessment of the efficiency of DNA in downstream process using polymerase chain reaction

  Genomic DNA extraction from bacterial cells involves processes normally performed in most biological laboratories. Therefore, various methods have been offered, manually and kit, but these methods may be time consuming and costly. In this paper, genomic DNA extraction of Pseudomonas aeruginosa was investigated using some laundry detergent brands available in Iran. Afterwards, efficiency of...

Author(s):   Reza Mirnejad, Hamid Babavalian, Mehrdad Moosazadeh Moghaddam, Samaneh Khodi and Fatemeh Shakeri  

January 2012

Evaluation of the effect of fat content of sunflower meal on rumen fungi growth and population by direct (quantitative competitive polymerase chain reaction) and indirect (dry matter and neutral detergent fibre disappearance) methods

  The major aim of this study was to evaluate the effect of fat content of sunflower meal (150 and 30 g fat /kg dry matter, high and low fat, respectively) on population, growth and activity of rumen anaerobic fungi by using direct (quantitative competitive polymerase chain reaction, QC-PCR) and indirect (dry matter (DM) and neutral detergent fibre (NDF) disappearance in rumen fungi media...

Author(s):   T. Mohammadabadi, M. Danesh Mesgaran, M. Chaji and R. Tahmasebi  

January 2012

Phenolic contents, antioxidant and α-glucosidase inhibition properties of Nepalese strain buckwheat vegetables

  The sprouts, microgreens and leafy greens of common and tartary buckwheat of Nepalese strain were compared for the phenolic contents and biological activity. The tartary buckwheat samples expressed higher total phenolic and flavonoid contents compared to the common buckwheat. The sprouts had the highest total phenolic contents (9333.48 ± 150.23 and 6976.21 ± 213.65 mgGAE/100g dw in tartary and...

Author(s):   Pankaja Sharma, Amal Kumar Ghimeray, Anup Gurung, Cheng Wu Jin, Ho Sik Rho and  Dong Ha Cho  

January 2012

Effects of dietary L-arginine on orthodontic tooth movement in rats

  The purpose of this study was to investigate the effects of dietary L-arginine as nitric oxide (NO) precursor on orthodontic tooth movement in rats. 36 male ten-week old Wistar rats were randomly divided into experimental and control groups. The experimental group received 2% (w/w) dietary L-arginine in drinking water six days before the insertion of springs to elevate their blood level. On the seventh day,...

Author(s):   Amir Mohammadi and Ramin Azar  

January 2012

Effects of aqueous extract of Triplochiton scleroxylon on some haematological parameters and blood glucose concentrations in non-diabetic rabbits

  Investigations on possible effects of aqueous extract of Triplochiton scleroxylon on some haematological parameters and glucose concentrations in non-diabetic rabbits were carried out. At least 100 ml of extract was administered daily for 15 days to test rabbits (New Zealand strain) through clean water troughs. Blood was drawn with the aid of sterile syringes from the large vein at the back of the...

Author(s):   Prohp, T. P. and Onoagbe, I. O.    

January 2012

Anthelmintic efficacy of pawpaw (Carica papaya) seeds in commercial layers

  The anthelmintic efficacy of the aqueous and crude extract of Carica papaya seeds was studied in 40 Isa Brown commercial layers infected naturally with nematodes. They were randomly divided into 4 groups: A, B, C and D with 10 birds per group. Group A birds were untreated, while groups B, C and D were treated orally with proprietary anthelmintic (piperazine 322 mg/kg body weight/day), powdery (300...

Author(s):   Ameen, S. A., Adedeji, O. S., Ojedapo, L. O., Salihu, T. and Fakorede, O. L.  

January 2012

Alpha 1 antitrypsin gene: A case-control study in chronic obstructive pulmonary disease

  Alpha-1 antitrypsin (AAT) deficiency is an inherited disorder that causes low levels of, or no AAT in the blood. The most common illness in adults with AAT deficiency is lung disease during the third and fourth decades of life. Most commonly, it is associated with chronic obstructive pulmonary disease (COPD). Mutations in the protease inhibitor (PI) gene, located on chromosome 14, are associated with this...

Author(s):   M. Bhattacharjee, B.G. Unni , S. Das, P. K. Baruah, P.sharma, D.Gogoi, M. Deka, S. B. Wann and P. G. Rao  

January 2012

Extraction of differential expressing aphid-resistance genes of sorghum (Sorghum bicolor L. Monech) and construction of suppression subtractive hybridization (SSH) library

  Experiments were conducted for the extraction of differential expressing aphid-resistance genes of sorghum (Sorghum bicolor L. Monech) in the experimental laboratories and fields of Hebei Agricultural University, China and Shenyang Agricultural University, Liaoning Province, China, during 2010 to 2011 and suppression subtractive hybridization (SSH) library was constructed. The seeds of two sorghum...

Author(s):   Khalid Hussain, Jifeng Qi, Hui Liu, Sun Quan, Jinhua Chang and Feng Lin  

January 2012

Endophytic Bacillus subtilis ZZ120 and its potential application in control of replant diseases

  An endophytic bacterial strain ZZ120 that was isolated from healthy stems of Prunus mume (family: Rosaceae) was identified as Bacillus subtilis based on biochemical and physiological assays and 16s rRNA, rpoB and tetB-yyaO / yyaR genes analysis. Both the culture filtrate and the n-butanol extract of strain ZZ120 showed strong growth inhibition activity in vitro against...

Author(s):   Hui Li, Xiaoxian Wang, Meizhe Han, Zhenzhen Zhao, Minqian Wang, Qin Tang, Changhong Liu, Brian Kemp, Yucheng Gu, Jinglei Shuang and Yarong Xue  

January 2012

Molecular identification versus local people’s information for accurate estimates of bushmeat utilization from the Serengeti ecosystem, Tanzania

  Sustainable wildlife management assumes thorough knowledge of the factors of importance for species population dynamics. In this study, we examined the diversity of wildlife species that are illegally harvested in the Serengeti ecosystem, Tanzania. A total of 124 bushmeat samples were obtained from hunters, middlemen and consumers in 79 sub-villages adjacent to the protected areas in western...

Author(s):   Stella Bitanyi, Gro Bjørnstad, Marit Nesje, Eblate M. Ernest, Robinson H. Mdegela and Knut H. Røed