African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 3 July 2012; 11(53)

July 2012

Determination of genotype differences through restriction endonuclease in Camels (Camelus dromedarius)

Tyrosinase gene or C locus has long been implicated in the coat colour determination.This gene a copper-containing enzyme located on chromosome 11q14.3 is expressed in melanocytes and controls the major steps in pigment production. In camel, C locus a restriction site provoked by the T variant of the mutation was used in a special restriction fragment length polymorphism analysis (PCR-RFLP) for genotyping of...

Author(s): Muhammad  Ghias  Uddin  Shah,  Monika  Reissmann,  Anas  Sarwar  Qureshi,  Horst  Jurgen Schwartz,  Muhammad  Shuaib  Khan,Tariq Ali   Ali  Zaman,    Kamran  Safdar , Khalid  Muhammad and Shakeeb  Ullah      

July 2012

Antioxidant capacity, nutritional and phytochemical content of peanut (Arachis hypogaea L.) shells and roots

This study evaluated antioxidant capacity, nutritional and phytochemical content of shells and roots of peanut (Arachis Hypogaea L.) for potential utilizations. Total dietary fiber, protein, ash and alkaloid per 100 g dry weight samples ranged from 58.8 to 78.2 g, 5.8 to 6.1 g, 6.6 to 21.7 g and 5 to 23.8 g, respectively. While total phenolic, total saponins and phytic acid per 100 g dry weight samples...

Author(s): Ee Wei Sim, Suk Yun Lai and Ying Ping Chang      

July 2012

Liming increases alfalfa yield and crude protein content in an acidic silty loam soil

A three year field trial examined the effect of three hydrated lime (HL, Ca(OH)2) rates (0, 1.5 and 3 Mg HL ha-1) on yield and quality characteristics of alfalfa on an acid soil (pHwater 1:2.5 4.7) in Western Serbia. Lime was applied only once. Total dry mass yields of a new planting alfalfa (Medicago sativa L.) increased up to 6500% by the treatments of HL, compared with the untreated control. Lime...

Author(s): Goran Dugalić, Boško Gajić, Nikola Bokan, Miodrag Jelić, Zorica Tomić and Ranko Dragović  

July 2012

Evaluating spring wheat cultivars for drought tolerance through yield and physiological parameters at booting and anthesis

Progress in wheat yields under drought conditions is rather a difficult task to achieve. The experiment was conducted in factorial design with 16 spring wheat cultivars grown under two irrigation regimes, non-stress and water-stress imposed at boot and anthesis growth stages. Water-stress significantly influenced the physiological and yield traits in both the growth stages, yet the reductions in most traits were...

Author(s): M. J. Baloch, Jim Dunwell, Mike Dennet, Zia-ul-hassan, I. Rajpar, Wajid Ali Jatoi and. N F. Veesar        

July 2012

Genetic diversity and molecular discrimination of wild tea plants from Yunnan Province based on inter-simple sequence repeats (ISSR) markers

To efficiently assess and discriminate wild tea germplasms, inter-simple sequence repeats (ISSR) were used to determine genetic relationships among 40 wild tea plants. A total of 275 bands were generated with 15 ISSR primers, of which 274 (99.6%) were polymorphic. The mean genetic similarity coefficient, the mean Nei’s gene diversity (h) and the mean Shannon’s information index (I) of tea cultivars were...

Author(s): Benying Liu, Xuemei Sun, Yungang Wang, Youyong Li, Hao Cheng, Changyun Xiong and Pingsheng Wang    

July 2012

Phytotoxic characterization of crude methanolic extract of Periploca aphylla

Periploca aphylla is traditionally used in the treatment of various ailments. Phytotoxic activity of crude methanolic extract of P. aphylla was tested on the germination of wheat seeds and on the growth of the germinated seedlings. In both the field and plate studies, the extract showed inhibitory effect on the germination of the growth of root and shoot of the seedlings. The inhibition was found to be...

Author(s): Rahmat Ali Khan, Noor Aslam Khan, Mustaq Ahmed, Muhammad Rashid Khan, Farid Ullah Khan, Abdus Saboor Shah and Mir Sadiq Shah        

July 2012

Outcrossing rate in olive assessed by microsatellite and inter simple sequence repeat (ISSR) markers

Olive is known to be an allogamous species. The aim of this study was to estimate the magnitude of cross-pollination rate using microsatellite simple sequence repeats (SSR) and inter simple sequence repeats (ISSR) molecular markers in olive genotypes. The DNA from maternal plants and 23 progenies of two different accessions, Ascolano USA and MGS GRAP541 were extracted and screened with two microsatellite and ten ISSR...

Author(s): Rafaeli Aparecida Vieira de Souza, Juliano Lino Ferreira, Francyane Tavares Braga, Patrícia Helena de Azevedo, Gustavo César Sant’Ana, Ana PaulaRibeiro, Aluízio Borém and Geraldo Magela de Almeida Cançado  

July 2012

Effects of plant growth regulators and photoperiod on in vitro microtuberization of potato (Solanum tuberosum L.)

In vitro microtuber production of potato (Solanum tuberosum L.) cvs. Sante and Savalan were studied on solid Murashige and Skoog (MS) basal medium applying different plant growth regulators 2,4-dichlorophenoxyacetic acid and benzylamino purine (2,4-D and BAP) and photoperiods. Cultures were exposed to 16, 8 and 16 h+utter darkness photoperiodic regimes. The experimental design,...

Author(s): Raheleh Ahmadzadeh Ghavidel, Ahmad Reza Bolandi,Hassan Hamidi andSetareh Foroghian

July 2012

Effect of 5-aminolevulinic acid on yield and quality of lettuce in sunlit greenhouse

The role of 5-aminolevulinic acid (ALA) as a precursor of chlorophyll and heme is well documented. Low concentration of exogenous ALA has been found to regulate plant growth and increase crop yield, but there is little information on how ALA influences the yield and quality of lettuce in sunlit greenhouse. Here, we report the effects of ALA on photosynthetic rate, yield and quality of lettuce in sunlit...

Author(s): Fuli Xu, Weiling Wang and Dan Yu      

July 2012

Screening and identification of cellulase producing yeast-like microorganisms from Brazilian biomes

The main goals of the present study included the screening and identification of cellulase producing wild yeasts, isolated from samples collected from different Brazilian biomes. They were selected according to their capabilities of degrading carboxymethyl cellulose (CMC) and micro-crystalline cellulose (SERVACEL®), as single carbon sources in solid medium. After the step of solid medium selection, yeast cells were...

Author(s): R. Goldbeck, C. C. P. Andrade, G. A. G. Pereira and F. Maugeri Filho        

July 2012

Karyotype and nucleic acid content in Zantedeschia aethiopica Spr. and Zantedeschia elliottiana Engl.

Analysis of karyotype, nucleic deoxyribonucleic acid (DNA) content and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were performed inZantedeschia aethiopica and Zantedeschia elliottiana. Mitotic metaphase in both species showed 2n=32. The chromosomes of both species were quite similar with medium length ranging from 1.55 ± 0.04 to 3.85 ± 0.12 µM in Z....

Author(s): Bimal Kumar Ghimire, Chang Yeon Yu, Ha Jung Kim and Ill Min Chung        

July 2012

Biochemical properties of oxidases of Yali pear

The biochemical properties of polyphenol oxidase (PPO), peroxidase (POD) andascorbate peroxidase (APX) from Yali pear were investigated. The optimum pH and temperature of three enzymes was 5.6, 4.0, 7.0 and 20, 40, 50°C, respectively. Enzyme kinetics results showed that the Michaelis constant (Km) and maximum velocity (Vmax) of PPO for catechol were 0.22 M and 1111 U/ml/min. The Km and Vmax values of POD for...

Author(s): Hui Zou#, Guangyuan Meng#, Ran Wei#, Yawei Zhang, Yilun Chen and Le Jia   #These authors contributed equally to this work.

July 2012

Evaluating the effect of some botanical insecticides on the citrus mealybug Planococcus citri (Risso) (Hemiptera: Pseudococcidae)

Planococcus citri (Risso) (Homoptera: Pseudococcidae), is one of the key pests of citrus. The use of chemical pesticides for a long time can cause many problems such as pesticide resistance, as well as having an adverse effect on the environment. The use of chemical pesticides needs to be replaced with non-chemical control methods. The effects of tondexir (pepper extract) and palizin (eucalyptus extract)...

Author(s):   Ahmadi, M., Amiri-Besheli, B. and Hosieni, S. Z.        

July 2012

Are specialized servers better at predicting protein structures than stand alone software?

This research study answers the question that technology is the best for predicting protein structures. Stand-alone software only depend on protein structure prediction algorithms, while web servers consult a number of other sources such as meta servers and protein data banks to produce a protein structure achieved through consensus.   Key words: Protein structure prediction, web servers, meta...

Author(s): B. N. Saeed and H. Q. Rabail      

July 2012

Cultivation of Pleurotus mushrooms in substrates obtained by short composting and steam pasteurization

This paper presents results of two experiments for cultivation of Pleurotus ostreatus,Pleurotus pulmonarius and Pleurotus eryngii grown with different formulations of grass and straw mixtures derived from agro-industrial residues. Cultivation was prepared through a number of approaches, such as short composting/pasteurization and axenic culture. In the first experiment, P. pulmonarius was...

Author(s): Félix G. de Siqueira, William P. Maciel, Emerson T. Martos, Gilvan C. Duarte, Robert N. G. Miller, Romildo da Silva and Eustáquio S. Dias      

July 2012

Aqueous ethanolic extract of Cochlospermum planchonii rhizome enhances spermatogenesis in male albino rats

Cochlospermum planchonii has numerous therapeutic benefits and is widely used in folklore medicine of many African countries. This study was designed to investigate the effects of aqueous ethanolic extract of Cochlospermum planchonii root on sperm characteristics of albino rats. Animals were assigned into four groups comprising a control, which received only distilled water and treatment groups at the...

Author(s): A. H. Abu, D. O. Ochalefu and A. Ibrahim