December 2013
‘Omics’ approaches in tomato aimed at identifying candidate genes for ascorbic acid accumulation in the fruit
Tomato (Solanum lycopersicum) is one of the most important vegetables in the world with significant importance for human health and nutrition. This species has long served as model system for plant genetics, development, physiology, pathology, and fleshy fruit ripening, resulting in the accumulation of many genetic and genomic resources. In addition, the tremendous development of high-throughput technologies, such as...
December 2013
Short hairpin RNA expression for enhancing the resistance of Bombyx mori (Bm) to nucleopolyhedrovirus in vitro and in vivo
A new paradigm of RNAi technology has been studied for enhancing the resistance to virus in plants and animals. Previous studies have shown that the Bombyx mori (Bm) U6 promoter based shRNA is an effective tool for inducing RNAi in Bombyx mori cell line. However, widespread knockdown and induction of phenotypes in Bm larvae have not been fully demonstrated. In this study, we examined Bm U6 promoter based shRNA...
December 2013
Composition of raw cow milk and artisanal yoghurt collected in Maroua (Cameroon)
The composition of milk is of most importance to the dairy industry and human health. This study was conducted to provide data on the composition of raw cow milk and artisanal yoghurt collected in Maroua (Cameroon). Milk and yoghurt samples were collected from 11 breeding sites and 12 producers in the city of Maroua, respectively. The following parameters were determined: pH, dry matter, ash, fat, lactose, total...
December 2013
Cloning of a vacuolar H+-pryophosphatase gene from emphemeral plant Olimarabidopsis pumila whose overexpression improve salt tolerance in tobacco
Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A. thaliana, it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. The vacuolar H+-translocating inorganic pyrophosphatase (V-H+-PPase) is an electrogenic proton...
December 2013
In vitro regeneration of selected Kenyan papaya (Carica papaya L.) lines through shoot tip culture
Papaya, an important fruit crop in Kenya is commercially propagated through seeds which lead to production of non-true-to-types plants. Conventional vegetative propagation methods are not carried out, hence, the need for micropropagation for mass multiplication of selected lines. An assessment for the effect of 6-benzylaminopurine (BAP) at 0.1, 0.5, 1.0 and 2.0 mg/l combined with α-naphthalene acetic acid (NAA) at...
December 2013
Seed yield and quality of pepper plants grown under salt stress
The effect of salinity on seed yield and quality of pepper plants were evaluated. Plants were grown in five salt levels (electrical conductivity, EC): 1.0, 1.5, 2.0, 4.0 to 6.0 dSm-1 in glasshouse. Seed yield was assessed by seed weight/fruit, seed weight/plant and individual seed weight. Seed quality was measured by germination, mean germination time, seedling growth and germination percentages after cold (10°C, 7...
December 2013
Physiological studies of Sclerotium rolfsii Sacc. causing collar rot of peppermint
In vitro studies were conducted on the effect of temperature, pH levels, carbon, nitrogen and amino acids on the mycelial growth and biomass production of Sclerotium rofsii Sacc. causing collar rot of mint. The results reveal that the growth of S. rolfsii was maximum at 30°C which was reduced significantly below 20°C and above 35°C. Of the pH levels tested, acetic pH (5.0) produced maximum mycelial dry...
December 2013
Effect of light and aeration on the growth of Sclerotium rolfsii in vitro
Sclerotium rolfsii is one of the devastating soil-borne plant pathogens which cause severe loss at the time of seedling development. It also causes leaf spots in several crops and wild plants. In this experiment, exposure of pathogen to different light period and aeration in order to assess the mycelial growth, biomass production, weight and number of sclerotia of S. rolfsii was done. Three-fourth area of three plates,...
December 2013
Production and characterization of antimicrobial active substance from some macroalgae collected from Abu-Qir bay (Alexandria) Egypt
The antimicrobial activity of three different macroalgal species [Jania rubens (Linnaeus) Lamouroux; Ulva fasciata Delile and Sargassum vulgare C. Agardh] belonging to Rhodophyta, Chlorophyta and Phaeophyceae, respectively, were collected seasonally in 2007 to 2008 from Abu-Qir bay (Alexandria, Egypt). The different macroalgal species were tested against pathogenic microbes such as Bacillus subtilis, Staphylococcus...
December 2013
Moroccan rock phosphate solubilization during a thermo-anaerobic grassland waste biodegradation process
In order to investigate the presence of thermo-tolerant rock phosphate (RP) solubilizing anaerobic microbes during the fermentation process, we used grassland as sole organic substrate to evaluate the RP solubilization process under anaerobic thermophilic conditions. The result shows a significant decrease of pH from 6.5 to 4.8, and solubilizing from 7 to 15.8% of the phosphorus from the RP in the reactors after 90 days...
December 2013
RAPD-PCR analysis of some species of Euphorbia grown in University of Baghdad Campus in Jadiriyah
This study attempts to identify species of Euphorbia (Euphorbia peplus, Euphorbia helioscopia, Euphorbia granulata and Euphorbia hirta) grown in University of Baghdad Campus in Jadiriyah and determine the genetic polymorphism among them by using DNA markers generated by polymerase chain reaction (PCR). Total genomic DNA of species studied was extracted from dry seeds by using commercial kit. Molecular analysis was...
December 2013
Identification of phytochemical components of aloe plantlets by gas chromatography-mass spectrometry
Aloe vera plants were collected from Blochestan, Iran and were transferred to tissue culture laboratory. Shoot tip explants were inoculated on solid MS medium supplemented with 0.5 mgl-1 benzyl adenine + 0.5 mgl-1 α-naphthalene acetic acid and sub-cultured on the same medium for plantlet production and propagation once every four weeks. After plantlets production, extracts of A. vera plantlet were analyzed by gas...
December 2013
The role of Nardostachys jatamansi against doxorubicin-induced toxicity in rats
This investigation elucidated the role of free radicals in doxorubicin-induced toxicity and protection by Nardostachys jatamansi (NJ). Adult male albino wistar rats were administered with doxorubicin (15 mg/kg; i.p.) and NJ (500 mg/kg, orally) for seven days. At the end of the experiment, following decapitation, heart and liver tissue samples were taken for histological examination, determination of malondialdehyde...
December 2013
Effect of potassium simplex optimization medium (KSOM) and embryo screening on the production of human lactoferrin transgenic cloned dairy goats
In this study, we produced cloned transgenic dairy goat based on dairy goat ear skin fibroblast as donor cells for nuclear transfer (NT), which were modified by human lactoferrin (hLF) gene. The developmental competence of NT embryos was compared with either between different embryo culture medium, potassium simplex optimization medium (KSOM) and tissue culture medium (TCM 199), or different classification of NT...
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