African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 4 December 2012; 11(97)

December 2012

Embryo implantation: Shedding light on the roles of ovarian hormones, cytokines and growth factors in the implantation process

  Implantation is a crucial step in mammalian reproduction, as it is a gateway to further embryonic development and successful pregnancy. Successful implantation requires coordinated interactions between the blastocyst and uterus. Uterine receptivity for embryo implantation is regulated by the ovarian hormones estrogen and progesterone. Some cytokines and growth factors play important roles in embryo...

Author(s): Huda Yahia Hamid and Md Zuki Abu Bakar Zakaria

December 2012

Genetic diversity study of indigenous cattle (Gir and Kankrej) population of Rajasthan using microsatellite markers

  The genetic diversity study of native Gir and Kankrej (Bos indicus) cattle populations were evaluated using nine microsatellite markers (ETH-225, CSRM-60, HEL-9, INRA-005, ETH-10, HAUT-24, BM1818, ILSTS-002 and ILSTS-006) suggested by FAO (ISAG). A total of 60 cattle were sampled from different places of local Rajasthan region. For each, 30 individuals were sampled. The mean number of observed and effective...

Author(s): Mona Upreti, Farah Naz Faridi, S. Maherchandani, B. N. Shringi and S. K. Kashyap

December 2012

Characterization and diversity assessment in coconut collections of Pacific Ocean Islands and Nicobar Islands

  Characterization and genetic diversity assessment among 30 conserved coconut accessions collected from Pacific Ocean Islands and Nicobar Islands for morphological traits, physico-chemical traits of tender nut water, leaf biochemical parameters and molecular characterization by using random amplified polymorphic DNA (RAPD) markers were undertaken at World Coconut Germplasm Centre, Andaman. Significant...

Author(s): Sankaran  M., Damodaran V., Singh D. R., Jai Sankar I. and Jerard B. A.

December 2012

Analysis of morphological traits in different host plants associated with resistance to Phenacoccus solenopsis- an invasive pest in Pakistan

  Phenacoccus solenopsis Tinsley (Pseudococcidae: Hemiptera) is a sucking insect pest and is polyphagous in nature. Twenty five (25) different host plants were screened against P. solenopsis and their physicomorphic traits were determined to correlate their role against pest population. Among the tested plant species, most favorable host plants of mealy bug on the basis of population...

Author(s):   Muhammad Rafiq Shahid, Muhammad Jalal Arif, Muhammad Aslam Khan, Amjad Ali, Anjum Suhail, Muhammad Shahid Iqbal, Muhammad Javaid, RehanaBibi, Asif Shehzad, Azhar Abbas Khan and Arif Muhammad Khan

December 2012

Isolation of Thiobacillus spp. and its application in the removal of heavy metals from activated sludge

  Two strains of Thiobacillus isolated from native excess activated sludge were identified as Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans by 16S rRNA gene sequencing and physiological-biochemical characteristics. Single and mixed cultures of the strains were used to carry out bioleaching for 9 days in order to remove heavy metals...

Author(s): Qiang Li, Cong Wang, Baobin Li, Cunmin Sun, Fei Deng, Cunjiang Song, and Shufang Wang

December 2012

Cellulase production from waste paper using Trichoderma species isolated from rhizospheric soil

  The recent interest in bioconversion of cellulosic wastes to value added chemicals has led to extensive studies on microorganisms capable of producing the enzyme. The possibility of using waste paper for the production of cellulase by Trichodermaspecies isolated from the soil was investigated using submerged fermentation technique. The fermentation temperature and pH were varied. Results indicated that...

Author(s): Damisa, D., Sule, E. I. and Moneme, S.

December 2012

Characterization and mechanisms of lipid metabolism in high-fat diet induced hyperlipidemia in Mongolian gerbil (Meriones unguiculatus)

  Rodent are common animal models for hyperlipidemia. In the present study, the differences in lipid metabolism between gerbils and rats were investigated. Feeding a high-fat diet led to a significant increase in serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDLC) and high-density lipoprotein cholesterol (HDLC) in gerbils, and were found in a time-dependent manner during...

Author(s): Hua-Zong Ying, Chen-huan Yu, Zhi-yuan Wang, Bing Yu, Jia-Na Zang  and Yue-huan Liu

December 2012

Photo-oxidation of a DNase from human small intestine

  The purified small intestine DNase (SIDNase) from human intestine was inactivated by the irradiation of the enzyme in the presence of photosensitizer dyes (methylene blue and rose Bengal) which lead to a specific destruction of 10 amino acid residues: three histidine, two serine, one tryptophan and four tyrosine residues. The other photo-sensitive amino acid residues are not appreciably affected. It is...

Author(s): Mukaram Shikara and Thorria Radam Marzoog

December 2012

Evaluation of the concentration of toxic metals in cosmetic products in Nigeria

  It has been shown that heavy metals toxicity to humans is as a result of long term or high level exposure to pollutants common in the environment including the air, water, food and numerous consumer products such as the cosmetics and toiletries. In this study, we assessed the levels of toxic metals in different cosmetic products sold at local shops in Lagos, Nigeria. The cosmetic items included thirty creams...

Author(s): Adepoju-Bello, A. A., Oguntibeju, O. O., Adebisi, R. A., Okpala, N. and Coker, H. A. B.

December 2012

Biochemical modulation in male specimens of Channa punctatus (Bloch) under different habitats and seasons

  Studies were undertaken to identify the changes in biochemical parameters including water content, lipid and protein in tissues of liver, testis and muscle of Channapunctatus under natural and captive conditions along with physico-chemical parameters for the period January to December, 2008. Result shows that values of lipid in captive condition in muscle, liver and testis ranged from 1 to 4,...

Author(s): C. P. Singh, R. N. Ram, Mohd. Danish and Anup Kumar

December 2012

Effects of somatic cell count on the gross composition, protein fractions and mineral content of individual ewe’s milk

  The aim of the research was to evaluate the effect of two somatic cell content (SCC) levels (<265,000 and >265,000 cells/ml) on ewe milk composition, protein fractions and mineral content. Samples were collected during two years, from three different ewe herds. Each herd was surveyed four times per year, one per season. For each survey more than 10 individual milk samples were collected during morning...

Author(s): Andrea Summer, Massimo Malacarne, Sandro Sandri, Paolo Formaggioni, Primo Mariani and Piero Franceschi

December 2012

Developing DNA barcoding (matK) primers for marama bean [Tylosema esculentum (Burchell) Schreiber]

  DNA barcoding is based on the premise that a short standardized DNA barcoding sequence can distinguish individuals of a species because the genetic variation between species exceeds that within species. Information on genetic variation of breeding materials helps to maintain genetic diversity and sustains long term selection gain. This information is a prerequisite for the genetic improvement of any plant...

Author(s): Takundwa M., Chimwamurombe P. M., Kunert K. and Cullis C. A.