African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 4 October 2012; 11(80)

October 2012

Complete mitochondrial genome of the ornate rock lobster Panulirus ornatus (Crustacea: Decapoda)

  The ornate rock lobster Panulirus ornatus is an important commercial lobster in the world. In this study, we successfully amplified and sequenced the complete mitochondrial genome of P. ornatus. The DNA of P. ornatus is 15680 bp, containing 13 protein-coding genes (PCGs), two ribosomal RNA genes, 22 transfer RNA genes, and 1 AT-rich non-coding region. The sequence arrangement...

Author(s):   Huafang Liang  

October 2012

Genetic diversity of Afzelia xylocarpa (Kurz) Craib in Vietnam based on analyses of chloroplast markers and random amplified polymorphic DNA (RAPD)

  Afzelia xylocarpa (Kurz) Craib is not only an endangered species in Vietnam, but also around the world. The wood of this tree species is very valuable as it is used to construct houses and high quality furniture. Habitat loss and exploitation of A. xylocarpa by man have threatened the population to such an extent that the number of mature trees of this species has dwindled to quite a low...

Author(s):   Nguyen Duc Thanh, Le Thi Bich Thuy and Nguyen Hoang Nghia  

October 2012

Detection of FXIII gene V34L and fibrinogen β-gene -455G/A polymorphisms among Saudi Arabia population via polymerase chain reaction-reverse hybridization technique

  FXIII gene Val34Leu variant appears to be associated with decreased risk of myocardial infarction and venous thromboembolism as well as with increased risk of intracerebral hemorrhage. Fibrinogen β-gene SNP -455G/A are associated with differences in the plasma levels of fibrinogen and severity of arterial disease. The aim of the present work was to study the prevalence of FXIII gene V34L and Fibrinogen...

Author(s):   Adel E. El-Tarras, Nabil S. Awad, Manal M. Said and Nahla Mitwaly  

October 2012

In vitro regeneration and morphogenesis in Phyllanthus niruri L., an anti-plasmodial herb

  We investigated the use of in vitro techniques for regeneration of adventitious shoots in Phyllanthus niruri, an anti-malarial plant as an initial effort towards its domestication. Fruits cultured on Murashige and Skoog (1962) basal medium (MS) supplemented with BAP had low germination (61%) due to seed coat imposed dormancy. The culture of nodal cuttings explants on BAP, kinetin or 2-isopentyl...

Author(s):   K. Adusei-Fosu, W. Elegba, C. Annor, G.Y.P. Klu and K. E. Danso,   

October 2012

Identification of Turnip mosaic virus isolated from canola in northeast area of Iran

  During March and April of 2011, 436 samples showing viral disease symptoms were collected from canola fields in the Khorasan Razavi province. The samples were tested by double-antibody sandwich (DAS)-enzyme linked immunosorbent assay (ELISA) for the presence of Turnip mosaic virus (TuMV). Among the 436 samples, 117 samples were found to be infected with TuMV. One of the infected...

Author(s):   Sabokkhiz M. A., Jafarpour B., Shahriari Ahmadi F. and Tarighi S.  

October 2012

Molecular diversity and distribution of arbuscular mycorrhizal fungi in karst ecosystem, Southwest China

Nested polymerase chain reaction and denaturing gradient gel electrophoresis (DGGE) was used to study the molecular diverstiy and distribution of arbuscular mycorrhizal fungi (AMF) from microhabitats of three different vegetation types in karst ecosystem, Southwest China. We found molecular diversity in all three microhabitat types was high. The highest values of biodiversity index (4.06) and species abundance (68) were...

Author(s):   Yuan Wei, Shi Jie Wang, Xiu Ming Liu and Tian Zhi Huang,  

October 2012

Isolation and screening of some medically important fungi from indoor environment: Studying the effect of some environmental and chemical factors on their growth and spore adhesion

  Isolation of some pathogenic fungi from indoor environment that may cause diseases to athletes was the goal of this work. The effect of different cloth materials and some environmental factors on the growth and adhesion of the isolated fungi as Aspergillus sydowii, Cochliobolus hawaiiensis, Cochliobolus lunatus, Epicoccum nigrum,Nigrospora oryzae, Penicillium...

Author(s):   Rania M. A. Abedin, Samy A. El-Aassar, Maha A. Hassan, Soheir R. Salemand Abeer M. Salama  

October 2012

Fuel specific consumption and emission analysis in a cycle diesel motor generator using diesel and biodiesel from waste frying oil blends

  One of the main fuels that can be helpful in emissions reduction when compared with diesel oil is the biodiesel. Moreover, it is a renewable fuel and may be obtained from different animal fat and vegetable oil. The purpose of this work was to evaluate fuel specific consumption and emission of exhaustion gases using the biodiesel produced from waste frying oil in a cycle diesel internal combustion engine...

Author(s):   Luiz Inácio Chaves, Samuel Nelson Melegari de Souza, Helton Aparecido Rosa, Reinaldo Aparecido Bariccatti, Carlos Eduardo Camargo Nogueira, Deonir Secco, Willian Tenfen Wazilewski, Angélica Buzinaro Avaci, Paulo Job Brenneisen, Marcelo José da Silva and Gustavo Veloso  

October 2012

Screening for actinomyces isolated from soil with the ability to inhibit Xanthomonas oryzae pv. oryzae causing rice bacterial blight disease in Vietnam

  Bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the major diseases in rice culture of Northen Vietnam, as well as other rice-growing regions of the world. In this study, we isolated and screened for actinomycete strains from Vietnam with the ability to inhibit Xoo isolates from northern Vietnam. From 90 actinomycete strains taken from soil in...

Author(s):   Phan Thi Phuong Hoa, Nguyen Duc Quang, Yayoi Sakiyama, Duong Van Hop, Dinh Thuy Hang, Trinh Hoang Ha, Nguyen Thi Van, Nguyen Thi Kim Quy and Nguyen Thi Anh Dao  

October 2012

Structural and functional analysis of seeligeriolysin O by homology modeling

  Seeligeriolysin O (LSO) is a cholesterol-dependant cytolysin of Listeria seeligeri. These toxins are produced by various species of Gram-positive bacteria, including members of the genera Streptococcus, Clostridium, and Listeria. Apart from the cytolytic, LSO has been reported to perform cytokine-inducing activity as well. The present study deals with the prediction of three...

Author(s):   Muhammad Ashfaq Khan, Javid Hussain and Roshan Ali  

October 2012

Improved production, purification and some properties of α-amylase from Streptomyces clavifer

  Improvement of the α-amylase productivity of Streptomyces clavifer was achieved through studying the effect of some nutritional factors and ultra violet (UV) mutagenesis. Modification in the formula of liquid starch medium by decreasing the concentration of soluble starch to 1%, increasing the corn steep liquor to 2%, and adding 0.75% glucose and 0.01% L-valine caused significant increase in...

Author(s):   Mahmoud Abdul Megead Yassien and Hani Zakarea Asfour  

October 2012

Misting cooling technique for protected culture of Oncidium orchids in subtropical regions

  Oncidium are planted in open protected culture or under shading nets in subtropical regions. Because of the lack of cooling techniques for the inside microclimates, the production period is limited, and the quantity and quality of flowers is unstable. Without ventilating, high humidity and temperature induces severe growth problems. In this study, mechanical ventilation fans with intermittent misting...

Author(s):   T. Shen and C. Chen  

October 2012

Anti-inflammatory and analgesic effects of coral reef associated gastropod, Trochus tentorium from Tuticorin coastal waters, Southeastern India

  The aim of this work was to investigate extensively, the biomedical potential of the mollusc Trochus tentorium which are abundantly associated with coral reef of the Tuticorin coastal water. The 100% acetone fraction of the gastropod tested for its analgesic effect on Swiss mice model and anti-inflammatory activity on albino rat showed promising results. T. tentorium at the...

Author(s):   Chellaram, C., Prem Anand T., Kuberan G, Alex John A, Priya G, and Arvind Kumar B  

October 2012

Growth inhibition of mouse embryonic stem (ES) cells on the feeders from domestic animals

  Mouse embryonic stem cells (mESCs) can be propagated in vitro on the feeders of mouse embryonic fibroblasts. In this study, we found growth inhibition of mESCs cultured on embryonic fibroblast feeders derived from different livestock animals. Under the same condition, mESCs derived from mouse embryonic fibroblast feeders were seen on the mass-like colonies and round or oval images, and more...

Author(s):   Hongguo Cao, Zhifei Xie, Yong Pu, Pan Yang, Ya Liu, Yunsheng Li, Fugui Fang, Zijun Zhang, Yunhai Zhang, Yong Tao and Xiaorong Zhang