African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 5 January 2012; 11(2)

January 2012

Probenazole treatment inhibits anthocyanins biosynthesis via salicylic acid and AtNPR1 signal pathway in Arabidopsis

  It has been found that anthocyanins were accumulated in Arabidopsis under drought or salt stress. In this study, such accumulation was found to be inhibited by external applied probenazole (3-allyloxy-1, 2-benzisothiazole-1,1-dioxide, PBZ), which is the active ingredient in oryzemate used for the protection of rice from Magnaporthe grisea (blast fungus). Microarray analysis indicated that...

Author(s):   Yang J. Xiao, Gao Jiong, Jin Yu, Zhang J. Jian and Kuai B. Ke  

January 2012

Detection of Streptococcus iniae and Lactococcus garvieae by multiplex polymerase chain reaction (PCR) in some rainbow trout farms of Iran

  Streptococcosis is one of the most important bacterial diseases in farmed salmonid fishes. Streptococcus iniae and Lactococcus garvieae are known as the major pathogens of streptococcosis and lactococcosis in the rainbow trout, Oncorhynchus mykiss. The present study accomplished the detection of the two mentioned bacteria in some rainbow trout farms in the west of Iran. A total of 50...

Author(s):   Firooz Fadaeifard, Hasan Momtaz, Ebrahim Rahimi and Ali Mirzakhani

January 2012

Association between VDAC1 mRNA expression and intracellular ATP levels of cultured L-02 hepatocytes during hexavalent chromium toxicity

  One way in which xenobiotics induce apoptotic cell death is to alter the selective permeability of the intracellular voltage-dependent anion channel (VDAC1) in the mitochondrial membrane. In this study, we explored the association between VDAC1 mRNA expression and mitochondrial function during hexavalent chromium compounds [Cr(VI)] treatment. Cultured L-02 hepatocytes were treated with 2, 4, 8, 16, or 32...

Author(s):   Yang Yuan, Li Peng, Hu Gong-Hua, Zhou Yu, Dai Lu and Zhong Cai-Gao  

January 2012

Exposure to red light, temperature and exogenous gibberellins influenced germination of some winter weeds

  Red light, temperature and gibberellins are well known for their capacity to induce higher germination in dormant seeds of several plant species. In the current study, we investigated the effect of various temperature (10, 13, 16, 19, 22 and 25°C) andgibberellic acid (GA3) and GA4+7 concentrations (0.1, 1, 10, and 100 uM) on germination of Poa annua, Alopecurus...

Author(s):   Dong-Sheng Tang#, Muhammad Hamayun#, Abdul Latif Khan, Samin Jan, Yasmin Nawaz, Muhammad Irshad, Young-Eun Na and In-Jung Lee

January 2012

Repellent effect of sirinol (garlic emulsion) against Lasioderma serricorne (Coleoptera: Anobiidae) and Tribolium castaneum (Coleoptera: Tenebrionidae) by three laboratory methods

  To alleviate insect pest problems in storage, synthetic pesticides are recommended;but their use may create toxicity to non-target organisms, development of resistance and residues in treated products. Under such circumstances, the need for research to find a safe, convenient, durable and economically logical method is necessary. The application of repellents could be considered as a new control...

Author(s):   Mojtaba Ghane Jahromi, Ali Asgar Pourmirza and Mohammad Hasan Safaralizadeh  

January 2012

A physiological evaluation of the enhanced osmotic stress tolerance of an asymmetric somatic hybrid introgression line SR3 between bread wheat and couch grass

  The cultivar, Shanrong No.3 (SR3) is the product of an asymmetric somatic hybrid between the bread wheat (Triticum aestivum L. cv. Jinan 177) and couch grass(Thinopyrum ponticum). After seedlings of SR3 and Jinan 177 were hydroponically subjected to osmotic stress, the accumulation of proline and sugars, the activities of a number of antioxidant enzymes and the relative...

Author(s):   Guangmin Xia, Li Yan, Xiang Xie, Zhenying Peng and Shuantao Liu,  

January 2012

Effects of NaCl salinity on maize (Zea mays L.) at germination and early seedling stage

  The response of eight maize hybrids against five different salinity levels namely 0, 60, 120, 180 and 240 mM) were studied at germination and early seedling stage. This investigation was performed as factorial experiment under completely randomized design (CRD) with three replications for each salinity level. Analysis of variance (ANOVA) showed that there were significant differences (P < 0.05) between...

Author(s):   Zahra Khodarahmpour, Mansour ifar and Mohammad Motamedi  

January 2012

Identification and antifungal activity of Streptomyces sp. S72 isolated from Lake Oubeira sediments in North-East of Algeria

  The aim of this study was to identify a new actinomycete isolate Streptomyces sp. S72 with antifungal activity from sediments of Oubeira’s Lake located at the North-East of Algeria. This isolate was identified based on a great variety of morphological, cultural, physiological and molecular characteristics analysis of 16S rDNA sequence. The test of antifungal activity for several pathogens...

Author(s):   Adel Ayari, Houda Morakchi and Kirane Gacemi Djamila  

January 2012

Isolation and characterization of rock phosphate solubilizing actinobacteria from a Togolese phosphate mine

  A phosphate mine from Togo, an original biotope rich in insoluble rock phosphate (RP), was explored for the presence of RP solubilizing actinobacteria. One hundred and fifty actinobacteria isolates originating from this mine was tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble RP as unique phosphate source. Only 29 isolates (19%) were able to weather RP in SMM medium....

Author(s):   Hanane Hamdali, , Koriko Moursalou, Gado Tchangbedji, Yedir Ouhdouch and  Mohamed Hafidi  

January 2012

Comparison of classifications of aptamers against Vibrio alginolyticus based on their primary and secondary structure

  As a novel method to detect the pathogen Vibrio alginolyticus, 45 aptamers were previously selected and tested. In order to better understand the properties of these aptamers, it was essential to classify these aptamers based on appropriate criteria. The primary structure of 45 aptamers against V. alginolyticus was analyzed by the software DNAMAN and these aptamers were classified into 11...

Author(s):   Jiang Zheng, Jumin Hao, Zhongbao Li, Qingpi Yan, Jun Wang, Fang Han, Yuanyue Li, Zhiqiang Lu and Yongquan Su  

January 2012

Purification and characterization of amidase from acrylamide-degrading bacterium Burkholderia sp. strain DR.Y27

  An amidase from a newly isolated acrylamide-degrading bacterium Burkholderia sp.strain DR.Y27 was purified to homogeneity by a combination of anion exchange and gel filtration chromatography. The purification strategy achieved 11.15 of purification fold and a yield of 1.55%. The purified amidase consisted of four identical subunits with a molecular weight 47 kDa and was active within the temperature...

Author(s):   Mohd Arif Syed, Siti Aqlima Ahmad, Norzila Kusnin and Mohd Yunus Abdul Shukor  

January 2012

Diversity of bacteria and archaea in the deep-sea low-temperature hydrothermal sulfide chimney of the Northeastern Pacific Ocean

  Our knowledge of the diversity and role of hydrothermal vents microorganisms has considerably expanded over the past decade, while little is known about the diversity of microorganisms in low-temperature hydrothermal sulfide chimney. In this study, denaturing gradient gel electrophoresis (DGGE) and 16S rDNA sequencing were used to examine the abundance and diversity of microorganisms from the exterior to the...

Author(s):   Xia Ding, Xiao-Jue Peng#, Xiao-Tong Peng and Huai-Yang Zhou

January 2012

Vegetative zonation patterns in depression and riparian wetlands of the Sanjiang Plain, Northeastern China

  The vegetation of depressional and riparian wetlands in the Sanjiang Plain of Northeastern China was studied to describe their vegetation composition and to quantify community differences. Reciprocal transplants were performed to test the importance of biotic factors in governing zonation. One hundred and seventy-two(172) sampling plots in depression and riparian wetland was used. Samples were classified...

Author(s):   Yanjing Lou, Xianguo Lu, Guoping Wang and Ming Jiang and Kuiyi Zhao

January 2012

Evaluation of coated urea for ammonia volatilization loss, nitrogen mineralization and microsite pH in selected soil series

  The increase in microsite soil pH due to surface application of urea to the soil is an important reason of ammonia volatilization loss and fast hydrolysis process. The use of Cu and Zn with biodegradable materials coatings were assumed to reduce ammonia volatilization losses by reducing soil pH and slowing down hydrolysis process. The present study was conducted to evaluate the effects of coated urea with the...

Author(s):   Nasima Junejo, Khanif, M. Y., Dharejo, K. A., Hazandy Abdul-Hamid and Arifin Abdu  

January 2012

Effects of salinity stress on growth, chlorophyll content and osmotic components of two basil (Ocimum basilicum L.) genotypes

  Salinity stress is a severe environmental factor limiting crop yield worldwide. To better understand salt stress responses in crop plants, we compared effects of salinity stress on growth, chlorophyll content and osmotic components in two basil genotypes. Seedling of two basil genotypes (B1 = Ociumum basilicm L and B2=Ociumum minimum L.) were grown in controlled environment in Hoagland...

Author(s):   Mostafa Heidari

January 2012

In vitro mecA gene transfer among Staphylococcus aureus in Malaysian clinical isolates

  A simple experiment was performed to observe in vitro transfer of mecA gene from methicillin-resistant Staphylococcus aureus, Malaysian clinical isolates, to methicillin-sensitive one. PCR-based method was used in combination with the selective antibiotic screening method to study the transfer of resistance among clinical isolates carrying different genetic markers. After...

Author(s):   Negar Shafiei Sabet, Geetha Subramaniam, Parasakthi Navaratnam and Shamala Devi Sekaran  

January 2012

Production, purification and characterization of tannase from Aspergillus tamarii

  The production of tannases by Aspergillus tamarii was evaluated in submerged cultures using tannic acid and gallic acid as substrates. Two tannases, designated as TAH I and TAH II were produced in gallic acid submerged cultures. TAH I, responsible for 70% of the total tannase activity was purified to apparent electrophoretic homogeneity with 18.35% yield. The enzyme is a homodimeric protein...

Author(s):   Andréa Miura da Costa, Marina Kimiko Kadowaki, Monique Caroline Minozzo, Cristina Giatti Marques de Souza, Cinthia Gandolfi Boer, Adelar Bracht and Rosane Marina Peralta

January 2012

Effect of enriched Brachionus plicatilis and Artemia salina nauplii by microalga Tetraselmis chuii (Bütcher) grown on four different culture media on the growth and survival of Sparus aurata larvae

  The growth, developmental stages and survival rates of Sparus aurata larvae fed with Brachionus plicatilis and Artemia salina nauplii enriched by microalgaTetraselmis chuii were studied. Two experiments were carried out; the first concerning with culturing the microalga (T. chuii) in four different media, then using these cultures for enrichment of the rotifer...

Author(s):   Hanan Mohamed Khairy and Heba Saad El-Sayed  

January 2012

Susceptibility of imipenem-resistant Pseudomonas aeruginosa to flavonoid glycosides of date palm (Phoenix dactylifera L.) tamar growing in Al Madinah, Saudi Arabia

  The fruits of the date palm (Phoenix dactylifera L.) are consumed throughout the world and are an important part of the diet in the Middle East. Dates at tamar stage contain a wide array of flavones, but little is known about the antimicrobial of flavonoid glycosides compounds in dates. In this study, we evaluated the antimicrobial of flavonoid glycosides extracted from the date fruits grown in Al...

Author(s):   Samy Selim, Sahar El Alfy, Meshref Al-Ruwaili, Amin Abdo and Soad Al Jaouni

January 2012

Depolymerization of chitosan by enzymes from the digestive tract of sea cucumber Stichopus japonicus

  A complex of enzymes was isolated in a preparation derived from the digestive tract of sea cucumber, Stichopus japonicus. Hydrolysis of chitosan using this enzyme preparation decreased its molecular weight (Mw), increased its water solubility and produced water-soluble chitosan (WSC). The conditions for hydrolysis were optimized to pH 6.0, temperature 45°C, 16 mg enzyme preparation (22.08 U of...

Author(s):   Dong-Rui Yao, Ming-Qian Zhou, Sheng-Jun Wu and Sai-Kun Pan  

January 2012

Free radical scavenging activities of pigment extract from Hibiscus syriacus L. petals in vitro

  The macroporous resin adsorption method was used to purify the pigment fromHibiscus syriacus L. petals. Through the comparison of the adsorption and desorption rates of six types of macroporous resins including AB-8, S-8, NKA-9, DM-130, D101 and 860021 to the pigment, 860021 resin was selected as the most appropriate resin to purify the pigment. The antioxidant capacities of the pigment extract (PE)...

Author(s):   Mingjiang Geng, Mingxin Ren, Zhenling Liu and Xiaojun Shang  

January 2012

Zinc availability of two wheat cultivars in soil amended with organic and inorganic Zn sources

  The nutrients and organic matter in organic amendments provide a valuable resource to agriculture, forestry and remediation of degraded lands. The objective of this research was to investigate the effects of two wheat cultivars (cv. Backcross and Alvand) on zinc (Zn) uptake in a loamy soil amended with organic and inorganic Zn sources. A factorial experiment using randomized complete blocks was used in three...

Author(s):   Tarighi,  H., Majidian, M. , Baghaie, A. H.  and Gomarian, M.  

January 2012

Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene

  Detection of Fusarium solani causal agent of wilt and rots in many plant species are difficult if based only on morphological characteristics. Beside this, morphological discrimination requires special skill and the expertise of taxonomists or specialists. To simplify the detection and discrimination of F. solani from other Fusariumspecies, end-point polymerase chain reaction...

Author(s):   Mohammad Arif, Shilpi Chawla, N. W. Zaidi, J. K. Rayar M. Variar and U. S. Singh  

January 2012

Detection of eight common β-globin gene mutation in thalassemia major patients using real time polymerase chain reaction (PCR)-high resolution melting and EvaGreen™ dye

  The aim of the present study was to determine the phenotype and genotype frequency of the most common β-globin mutations among the thalassemia major (TM) patients, using high-resolution melting (HRM) curve analysis as a novel, reliable and rapid scanning method. In this descriptive-analytical study, real time polymerase chain reaction (PCR) was applied besides HRM assay in the same...

Author(s):   Seyed Jalal Marashi, Sima Ataollahi Eshkoor, Mir saed Mirinargesi, Mohammad Reza Sarookhani, Asmah Bt. Rahmat and Patimah Bt. Ismail  

January 2012

Inhibition of Microcystis aeruginosa and microcystin-LR with one algicidal bacterium isolated from a eutrophic lake

  Toxic Microcystis aeruginosa blooms have been increased in freshwater body in China in recent years. The discovery of algicidal bacteria has brought renewed attention in the treatment of the harmful algae blooms (HABs). In this study, one strain of algicidal bacterium number SDK2 was selected from a eutrophic lake and its inhibition effects on M. aeruginosa and microcystin-LR...

Author(s):   Ruimin Mu, Guixia Ma,Cuizhen Sun and Jingjing Jia

January 2012

Comparative homology modeling of human rhodopsin with several templates of bovine rhodopsin

  Rhodopsin is a visual pigment, which belongs to G-coupled protein receptor (GPCR) family, present in rod cells of retina. The molecular structure of rhodopsin has been studied by cryo-electron microscopic, Nuclear Magnetic Resonance (NMR) and X-ray crystallographic techniques in bovine. A humble effort has been made to identify and remove the loopholes in the existing structure (1EDS) and model the...

Author(s):   Shagufta Kanwal, Sumaira Nishat, Muhammad Irfan Khan, Shaukat Iqbal Malik and Khalid Akhtar  

January 2012

Analysis of Iranian isolates of Fusarium solani using morphological, pathogenicity and microsatellite DNA marker characterization

  Many economically important phytopathogenic species are nested within Fusarium solani species complex. Since most of them lack Latin binomials, morphological and molecular phylogenetic analyses were conducted on strains selected to represent the known pathogenic and genetic diversity of this pathogen. To evaluate methods used in classifying strains of this pathogen, 33 F. solani isolates...

Author(s):   Morid Bahar and Hajmansoor Shahab  

January 2012

Up-regulation of Robo1 in dorsal root ganglia after sciatic nerve transection in rats

  Roundabout (Robo) receptors have many important roles including mediating the migration of the growth cone and neurons, promoting axonal growth and development of nerve fascicles, and guiding the growth direction of central nervous system by binding Slit. To better understand the role of Robo in peripheral nervous system, this study investigated the expression profile of Robo1 in the dorsal root...

Author(s):   Lin-Feng Zheng,, Su-Juan Zheng, Zhi-Jian Ma, Yuan-Zhong Xu, Xi-Nan Yi and Gui-Xiang Zhang  

January 2012

Identification and isolation of gene differentially expressed on scrotal circumference in crossbreed bulls

  Differential display reverse transcription polymerase chain reaction (DDRT-PCR), reverse northern blot analysis and real-time polymerase chain reaction (RT-PCR) were used to identify tissue-specific expression genes in scrotum of differential scrotal circumference bulls and the function of these specific genes on the development of the bull’s scrotum was analyzed in this study. The results...

Author(s):   Xue Gao# , Ming Yan Shi,#, Zheng Rong Yuan, Ruo Yu Chen, Zheng Kui Zhou, Jiao Li, Jun Ya Li , Hui Jiang Gao and Shang Zhong Xu

January 2012

Chemical composition, cytotoxicity and antioxidant activities of the essential oil from the leaves of Citrus aurantium L.

  The species of the genus Citrus (Rutaceae) have been widely used in traditional medicine. In this study, the essential oil was extracted from the leaves of Citrus aurantium and its cytotoxicity effect on six tumor cell lines and a normal cell line was studied. Furthermore, antioxidant potential of the oil was tested by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, hydrogen...

Author(s):   Mohammad-Bagher Majnooni, Kamran Mansouri, Mohammad-Bagher Gholivand, Ali Mostafaie, Hamid-Reza Mohammadi-Motlagh, Nazanin-Sadat Afnanzade, Mir-Mehdi Abolghasemi and Marzieh Piriyaei

January 2012

Erythroblast cell expansion as a marker for disease severity in b0-thalassemia/Hb E disease

  b0-Thalassemia/Hb E disease is extremely common in many parts of Southeast Asia.  In Thailand, there are believed to be approximately 100,000 people living with this disease which can present as a life threatening anemia syndrome dependent on regular blood transfusions. Determination of the state of presentation of the disease (mild, moderate or severe) is based upon a number of clinical and...

Author(s):   Amporn Leecharoenkiat, Tirawat Wannatung and Duncan R. Smith  

January 2012

Evaluation of antibacterial and antifungal activity of root and root callus extracts of Trianthema decandra L.

  Trianthema decandra L. root and root callus extracts of different solvents viz.,petroleum ether, chloroform, ethyl acetate and ethanol were tested against both Gram positive and Gram negative bacteria and also against Fusarium spp. Root callus extract of chloroform and ethanol showed significant activity against  Bacillus subtilis, B. cereus. Staphylococcus...

Author(s):   Mohammadreza Radfar, M. S. Sudarshana, H. U. Kavitha, S. Satish and M. H. Niranjan  

January 2012

Analysis of epidermal growth factor receptor in histopathologically tumor-free surgical margins in patients with oral squamous cell carcinoma

    Oral squamous cell carcinoma (OSCC) is the most common oral malignancy. The prevalence of OSCC has been reported in more than 90% of all oral cancers. The main treatment of OSCC is complete removal of the tumor. Nevertheless, treatment is not always successful. Approximately 40% of treatment failure rate is seen in patients with OSCC, the most common cause of which is local or regional lymph node...

Author(s):   Sepideh Vosoughhosseini, Mehrdad Lotfi, Ashraf Fakhrjou, Amirala Aghbali, Monir Moradzadeh, Mahmood Sina and Parya Emamverdizadeh  

January 2012

Hydrochlorothiazide increases interleukin-1 beta (IL-1β) secretion by peripheral blood mononuclear cells in healthy subjects

  Recent study shows close relationship between hypertension and inflammation. The concentration of inflammatory mediators is increased in patients with essential hypertension. Angiotensin II (Ang II) may contribute to inflammatory process. Previous studies showed that individuals with essential hypertension had increased interleukin-1beta (IL-1β) secretion by peripheral blood mononuclear cells (PBMCs) and...

Author(s):   Nemati Farkhondeh and Dehpour Abbas Ali  

January 2012

Effects of increasing dietary protein levels on growth, feed utilization and body composition of Heterobranchus longifilis (Valenciennes, 1840) fingerlings

  The effect of different dietary protein levels on growth performance and on feed utilization of catfish (Heterobranchus longifilis) fingerlings was carried out in aquaria. Five dietary protein levels 25, 30, 35, 40 and 45% were tried in triplicates. The result shows that 25% protein is too limited to ensure good growth and also the growth rate, weight gain and nitrogen metabolism increased...

Author(s):   Athanase K. Otchoumou, Célestin M. Blé, Olivier A. Etchian, Yao L. Alla, Sébastien L. Niamké and Jacques K. Diopoh  

January 2012

The effects of three methods of synchronization on estrus induction and hormonal profile in Kalkuhi ewes: A comparison study

  Due to the importances of estrus synchronization in sheep reproduction and fertility, the present trial had been carried out to compare the three methods of estrus synchronization in ewes during breeding season. 60 cycling, fat-tailed Iranian Kalkuhi ewes were randomly allocated in equal numbers (n = 20) to 3 treatment groups. In two treatment groups, sponges...

Author(s):   Hamed Naderipour, Jafar Yadi, Ali GhaziKhani Shad and Mohammad Ali Sirjani