African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 5 July 2012; 11(54)

July 2012

Genetic diversity and population structure of cotton (Gossypium hirsutum L. race latifolium H.) using microsatellite markers

Cotton (Gossypium spp.) is the world’s leading natural fiber crop and is cultivated in diverse temperate and tropical areas. In this sense, molecular markers are important tools for polymorphism identification in genetic diversity analyses. The objective of this study was to evaluate genetic diversity and population structure in tetraploid cotton (Gossypium hirsutum L. race latifolium H.)...

Author(s): Leonel Domingos Moiana, Pedro Soares Vidigal Filho, Maria Celeste Gonçalves-Vidigal, Giselly Figueiredo Lacanallo, Marta Zulema Galván, Luiz Paulo de Carvalho, Manuel Pedro Maleia, Cynthia Maritza Pacheco, Tiago Ribeiro, Hugo Zeni Neto and Gislayne Kelly Coimbra        

July 2012

Agricultural importance of algae

Algae are a large and diverse group of microorganisms that can carry out photosynthesis since they capture energy from sunlight. Algae play an important role in agriculture where they are used as biofertilizer and soil stabilizers. Algae, particularly the seaweeds, are used as fertilizers, resulting in less nitrogen and phosphorous runoff than the one from the use of livestock manure. This in turn, increases the quality...

Author(s): Abdel-Raouf N., Al-Homaidan A. A. and Ibraheem I. B. M.,        

July 2012

Etiology of hepatitis G virus (HGV) and hepatitis type C virus (HCV) infections in non-Hodgkin’s lymphoma patients in Southern Iran

Hepatitis G virus (HGV) and hepatitis type C virus (HCV) may implicate malignant lymphoma including non-Hodgkin’s lymphoma (NHL) for inducing the proliferative process of lymphocytes. In this study, the molecular and serologic prevalence of HGV and HCV infections was evaluated in patients with NHL and compared with thecontrols. In this cross sectional study, ethylene diamine-tetra acetic acid...

Author(s): Abbass Ali Rezaeian, Ramin Yaghobi, Mahdi Roshan Nia, Mitra Mirzaee,Mani Ramzi, and Marjan Shaheli        

July 2012

Association between polymorphisms of SLC6A3 and DRD1 genes and autism among Saudi Arabia Taif population using PCR-restriction fragment length polymorphism (PCR- RFLP)

The prevalence of autism in Saudi Arabia is 18 per 10,000, higher than the 13 per 10,000 reported in developed countries. The etiology of autism is still not completely understood. Different studies support the involvement of dopaminergic neurotransmitter system in the etiology of autism. Several lines of evidences suggest the role of some dopamine related genes, such as DRD1 and SLC6A3 in the etiology of autism. The...

Author(s): Adel E. El-Tarras, Nabil S. Awad, Nahla Mitwaly, Adnan A. Alsulaimani and Manal M. Said      

July 2012

Genetic diversity of five goat breeds in China based on microsatellite markers

The genetic diversity of five goat breeds in China was surveyed using 15 microsatellites. The five goat breeds included Tangshan dairy goat (TSD), Liaoning cashmere goat (LNC), Nanjiang yellow goat (NJY), Chengde polled goat (CDP) and Leizhou black goat (LZB). The mean polymorphism information content value (PIC) of the populations ranged from 0.6606 to 0.8405. The mean heterozygosity (H) of the populations ranged from...

Author(s): Liying Zhang, Qingfang Yang, Xianglong Li, Rongyan Zhou and Lanhui Li

July 2012

Detection, cloning and bioinformatics analysis of vip1/vip2 genes from local strains of Bacillus thuringiensis

Bio-insecticides based on the spore forming bacterium, Bacillus thuringiensis (Bt) have been used for commercial scale for the past 40 years. Bt is a Gram-positive soil bacterium that forms insecticidal crystal proteins (ICPs) during sporulation; it has been characterized as an insect pathogen. Vegetative insecticidal protein (VIP) is a newly discovered family of toxin protein isolated from Bt. A hundred...

Author(s): Osman, G. H.,

July 2012

Antioxidant activity influenced by in vivo and in vitro mutagenesis in sugarcane (Saccharum officinarum L.)

The antioxidant potential (1,1-diphenyl-2-picrylhydrazyl (DPPHº)-scavenging activity) of in vitro regenerated and induced mutant sugarcane (Saccharum officinarum L.) was investigated. Efficient callus induction and shoot regeneration were induced in bud explants when incubated on Murashige and Skoog (MS) medium supplemented with different plant growth regulators (PGRs). Best callogenesis was observed...

Author(s): Roshan Zamir, Shahid Akbar Khalil, Syed Tariq Shah, Nisar Ahmad and Saima

July 2012

Genetic diversity of Ustilago scitaminea Syd. in Southern China revealed by combined ISSR and RAPD analysis

The polymorphism and similarity relationships among 35 mating-type isolates ofUstilago scitaminea collected from Southern China were determined with random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses. These fungal isolates were collected from 16 sugarcane cultivars including F134 that is resistant to the physiological race 1 but susceptible to the race 2 of U....

Author(s): Wankuan Shen, Pinggen Xi, Minhui Li, Rui Liu, Longhua Sun, Zide Jiang and Lianhui Zhang

July 2012

In vitro plant regeneration of Esmeralda clarkei Rchb.f. via protocorm explant

Esmeralda clarkei is an epiphytic native orchid species of Nepal growing under medium amount of light with fragrant flowers. Reliable protocols for in vitro plant regeneration of E. clarkei via protocorm explants were developed. Protocorms obtained from in vitro germinated seeds cultured on Murashige and Skoog's (MS) medium supplemented with 6-benzylaminopurine (BAP) and...

Author(s): Paudel, Mukti Ram and Pant, Bijaya

July 2012

Ethanol production from tropical sugar beet juice

Starch and sugar resources have been extensively researched to find a suitable renewable source of energy to supplement the world’s ever increasing demand for energy while also abating global warming by stemming the addition of earthbound carbon dioxide into the atmosphere. Sugar beet has been used as a source for sugar production for some time, but its development as a large scale agricultural crop in South...

Author(s): S. Marx, J. Brandling and P van der Gryp

July 2012

Lurking threat of bird flu from migratory and non migratory birds in and around Islamabad Capital Territory, Pakistan

During extreme months of winter, December and January, every year, the migratory birds from Siberia travel to Indus Valley and other parts of Indo-Pakistan subcontinent. These migratory birds include, ducks, geese, swans and the water fowls. These birds are known to carry all avian influenza viruses and by shedding virus, they are assumed to be vector. Samples were collected from migratory and non migratory wild birds...

Author(s): Zahida Fatima, Muhammad Athar Khan, Mansur ud Din Ahmad, Abdul Ahad, Zubair Anwar Muhammad Hassan Mushtaq and Altaf mahmood

July 2012

Molecular and phenotypic characterization of Lactobacillus curvatus isolated from handmade Brazilian salami

17 Lactobacillus curvatus and one Lactobacillus brevis isolate were obtained from salami. The isolates were previously selected according to the desirable characteristics regarding technological criteria. Identification was based primarily on the biochemical carbohydrate assimilation profile, and later by amplified 16S rDNA restriction analysis (ARDRA). Due to inconsistencies in the results found by...

Author(s): César Milton Baratto, Jane Mary Lafayette Neves Gelinski, Jaqueline Debastiani and Marco Antonio Dalbó

July 2012

Antioxidant activity of eight plants consumed by great apes in Côte d’Ivoire

Oxidative stress is an aggravating factor involved in a number of pathologies. The source and mobilization of antioxidant compounds are a challenge for the public health sector and new approaches are needed to assess and identify the main sources of antioxidants. Monkeys and great apes are considered to tolerate the Simian immunodeficiency virus (SIV) infection and other diseases. The current study aimed at screening...

Author(s): AHOUA Angora Remi Constant, KONE Mamidou Witabouna, KONAN Amoin Georgette, TRA Bi Fézan Honora and BONFOH Bassirou

July 2012

Effect of vitamin B12 addition to extenders on some physicochemical parameters of semen in crossbred rams

The aim of this study was to examine the effect of different doses of vitamin B12 on some physicochemical parameters and antioxidtive enzyme activities in crossbreed rams semen during storage at 5°C. Semen samples were collected from eight crossbreed rams, evaluated and pooled at 33°C. Fresh semen was diluted with a Tris-based extender containing 0, 1, 2 and 3 mg/mL vitamin B12 and was cooled at 5°C. In both...

Author(s): R. Asadpour, M. M. Pourseif , G. Moghadam, R. Jafari, H. Tayefi and H. Mahmodi

July 2012

Correlation between morphological and biological characteristics of mesenchymal stem cells and hepatocytes derived from rat mesenchymal stem cells in vitro

Hepatocyte dysfunction with the possibility of eventual organ failure is created from most liver diseases. Images of cell morphology can be obtained nondestructively using a conventional inverted microscope. Therefore, this study attempted to investigate several morphological parameters of mesenchymal stem cells (MSCs) and MSCs-derived hepatocytes, in vitro, and their correlation with biological activities. Our...

Author(s): Mohammad Hadi Bahadori, Bahram Soltani, Abtin Heidarzadeh, Fatemeh Ghasemian and Shervin Ghadarjani

July 2012

Heavy metal pollution in benthic fishes from Kiri Dam in Guyuk local government area of Adamawa State, Nigeria

Heavy metal concentrations in lungfish (Polypterus annectens), African carp (Heterotis niloticus) and catfish (Clarotes laticeps) in Kiri Dam in the Guyuk local government area, Adamawa State, Nigeria were studied. The concentrations of lead (Pb), cadmium (Cd), copper (Cu), zinc (Zn), nickel (Ni) and iron (Fe) were determined. These metals concentrations were measured by inductively coupled plasma-optical emission...

Author(s): Milam, C., Maina, H. M., Onyia, L. U. and Ozoemena, P. E.