African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 5 June 2012; 11(45)

June 2012

Genetic variation among Iranian oaks (Quercus spp.) using random amplified polymorphic DNA (RAPD) markers

Quercus is one of the most important woody genera of the Northern hemisphere and considered as one of the main forest tree species in Iran. In this study, genetic relationships in the genus Quercus, using random amplified polymorphic DNA (RAPD) was examined. Five species, including: Quercus robur, Quercus macranthera, Quercus infectoria, Quercus magnosquamata and Quercus libani were...

Author(s): Maryam Ardi, Fatima Rahmani and Abbas Siami

June 2012

Molecular characterization of traditional and improved rice cultivars based on random amplified polymorphic DNAs (RAPDs) markers

Information of genetic similarities and diversity among superior rice cultivars is needed for potential rice breeding programs and origin of plant lines. Genetic polymorphism and relationships among 35 commercial varieties and primitive cultivars of Rice (Oryza sativa L.) were established by using random amplified polymorphic DNAs (RAPDs) primers. Polymerase chain reaction (PCR) with 14 primers produced a total of...

Author(s): Sadia Tehrim, Zahida Hassan Pervaiz and M. Ashiq Rabbani

June 2012

A simple and rapid DNA extraction method from leaves of grapevine suitable for polymerase chain reaction analysis

The genomic grapevine (Vitis vinifera L.) DNA extraction is difficult because of secondary metabolites that interfere with DNA isolation procedures and subsequent applications. We developed a simple, rapid and efficient method for the extraction of genomic DNA from asymptomatic and pathogen-infected grape leaves. The protocol reported, based on a modified cetyl trimethylammonium bromide (CTAB) extraction procedure,...

Author(s): Sandra Lo Piccolo, Antonio Alfonzo, Gaetano Conigliaro, Giancarlo Moschetti, Santella Burruano and Amalia Barone

June 2012

Effect of humin-urea-rock phosphate amendment on Bekenu series (Tipik Tualemkuts) soil

Application of crude humin with urea and Egyptian rock phosphate (ERP) may reduce ammonia loss from urea. This study was conducted to determine the effect of mixing crude humin with urea and ERP on ammonia volatilization and selected soil chemical properties. Crude humin from four different composts was mixed with urea and ERP and ammonia loss from these treatments were evaluated using the closed-dynamic air flow...

Author(s): P. Palanivell, K. Susilawati, O. H. Ahmed and Nik M. Majid

June 2012

Determination of antioxidant property from some medicinal plant extracts from Thailand

The evaluation of antioxidant property, total phenolic compounds and pigment content of 8 medicinal plants including: Gynostemma pentaphyllum Thunb., Camellia sinensisKtze., Cymbopogon citratus Stapf., Centella asiatica (L.) Urban., Andrographis paniculata (Burm.) Wall. ex Nees, Thunbergia laurifolia Lindl., Murdania loriformis(Hassk.) Rolla Rao et...

Author(s): Klomsakul Pongsathorn, Pumjumpa Duangporn, Khunpratum Sireethon and Chalopagorn Pornchanok

June 2012

Isolation and characterisation of a banana CYCD2;1 gene and its over-expression enhances root growth

Stimulation of the cell cycle by over-expression of Arabidopsis CyclinD2;1 enhanced growth in tobacco and rice, but not Arabidopsis. To broaden the range of species and to improve an important crop species for developing countries, a CyclinD2;1homologue was isolated from an East African highland banana cultivar ‘Nakasabira’ (Musa spp., AAA group) and...

Author(s): David Talengera, Gerrit T.S. Beemster, Wilberforce K. Tushemereirwe and Karl Kunert

June 2012

Effect of polyethylene glycol and mannitol on somatic embryogenesis of pigeonpea, Cajanus cajan (L.) Millsp.

A protocol for high frequency somatic embryogenesis (SE) of pigeonpea, Cajanus cajan (L.) Millsp. was worked out. The age of the source seedlings for explants and type of explants were found to influence the callusing response. Concentration of phytohormones and growth factors were optimized for developing embryogenic callus consisting of globular cells with dense cytoplasm. Embryo axis or callus derived from...

Author(s): Maluventhen Viji, Pichamuthu Maheswari, Thirupathi Karuppanapandian and Kumariah Manoharan

June 2012

Significance of secondary metabolites and enzymes secreted by Trichoderma atroviride isolates for the biological control of Phomopsis canker disease

Phomopsis theae Petch, the causative agent of Phomopsis canker diseases, is one of the major constrain in tea plants. The present study deals with development of efficient biological control agent against canker based on the secretion of several secondary metabolites and defense enzymes under in vitro conditions. Among the isolates evaluated, the antifungal compounds obtained from Tv1 Trichoderma...

Author(s): S. Anita, P. Ponmurugan and R. Ganesh Babu

June 2012

Establishment of in vitro plant regeneration system for Chimonanthus praecox (L.)

An efficient protocol for plant regeneration of Chimonanthus praecox (L.) Link, was developed using leaves from seedlings of seeds. Shoots induction were influenced by cytokinins and nodal positions. The results show that the highest callus induction frequency was obtained on MS medium supplemented with 2.0 mg l−1 6-benzyladenine (6-BA) and 0.9 mg l−1 1-naphthalene-acetic acid...

Author(s): Mingxiao Zhao, Guoqiang Fan and Xiaoshen Zhang

June 2012

Suitability of various plant derived gelling agents as agar substitute in microbiological growth media

Eleven putative gelling agents were investigated as agar substitutes. These included arrowroot (Maranta arundinaceae), coconut powder (Cocos nucifera), corn flour (Zea mays var. amylacea), gel rite (a water-soluble polysaccharide produced bySphingomonas elodea), glue (Cyanoacrylates), katira gum (Cochlospermum religiosum), guar gum (Cyamopsis tetragonolobus L.), isubgol husk (Plantago ovata), pectin and rice (Oryza...

Author(s): Abdul Mateen, Shaukat Hussain, Shams Ur Rehman, Basharat Mahmood, Muhammad Aslam Khan, Abdul Rashid, Majid Sohail, Muhammad Farooq, S. Jawad Ahmed Shah

June 2012

Potential of Azotobacter vinelandii Khsr1 as bio-inoculant

The present study deals with the isolation and characterization of Azotobacter vinelandii Khsr1 from roots of the weed, Chrysopogon aucheri, commonly known as golden beard grass indigenous to Khewra salt range, Pakistan and its evaluation as bio-inoculant. The population of the isolate varied from 107 to 108 cfu/g fresh weight of root. The preliminary identification of the isolate was made on...

Author(s): Irum Naz, Asghari Bano, Bushra Rehman, Simab Pervaiz, Mazhar Iqbal, Ambrin Sarwar and Farah Yasmin

June 2012

Combined effect of hormonal priming and salt treatments on germination percentage and antioxidant activities in lettuce seedlings

Hormonal priming is a pre-sowing treatment that improves seed germination performance and stress tolerance. To understand the physiology of hormonal priming and its association with post priming stress tolerance, we investigated the effect of hormonal priming with increasing gibberellic acid (GA3) concentrations (0, 3, 4.5 and 6 mM) on seedling growth and antioxidant system in lettuce. Germination percentage was higher...

Author(s): Mahmoudi Hela#, Zargouni Hanen#, Tarchoune Imen#, Baatour Olfa, Nasri Nawel, Ben Massoud Raouia, Zaghdoudi Maha, Abidi wissal, Huang Jun, Hannoufa Abdelali, Lachaâl Mokhtar and Ouerghi Zeineb

June 2012

An efficient callus initiation and direct regeneration of Stevia rebaudiana

The experiment was carried out to establish an efficient callus induction system ofStevia rebaudiana from a variety of explants as well as direct regeneration from nodes. Callus initiation was done to extract secondary metabolites. However direct regeneration is essential for rapid large-scale clonal propagation. Among leaf, node and inter-nodal explants, the best callus initiation performance was observed by...

Author(s): Saif U. Sikdar, Nayem Zobayer, Fazle Azim, M. Ashrafuzzaman and Shamsul H. Prodhan

June 2012

Screening and characterization of petroleum-degrading bacterium

Petroleum-degrading bacterium JY6 was isolated from petroleum-contaminated soils in DaQing oil field. It was identified as Bacillus cereus based on its morphological, physiological and biochemical characteristics, and analysis of its 16SrRNA gene. Biodegradation function of petroleum and oil degradation rates were studied, including different pH value, culture temperature, oxygen etc. Results show the optimum...

Author(s): WANG Gang, GUO MingZhu and CHEN Guang

June 2012

Heavy metal removal from aqueous solutions by sorption using natural clays from Burkina Faso

The acid-base properties of two raw and purified mixed clays from Burkina Faso were studied, as well as their potential to remove copper(II), lead(II) and chromium(III), and thereby their ability to be used to purify water from heavy metals. The purification procedure of the clays involved removal of carbonates, iron oxides and organic matter. A determination of the elemental composition of the mixed clays revealed the...

Author(s): S. Pare, I. Persson, B. Guel, D. Lundberg, L. Zerbo, S. Kam and K. Traoré

June 2012

Purification and characterization of a thermostable cyclodextrin glycosyltransferase from Thermoanaerobacter sp. P4

Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) from a thermophilic anaerobic bacterium, Thermoanaerobacter sp. P4, was purified by ammonium sulfate precipitation followed by α-cyclodextrin epoxy activated-sepharose 6B column chromatography. Enzyme was purified 141 fold and had the specific activity of 143.8 U/mg proteins. Purification yields after ammonium sulfate precipitation and affinity...

Author(s): Ayse Avci and Sedat Dönmez

June 2012

Co-administration of Vitamins E and C protects against stress-induced hepatorenal oxidative damage and effectively improves lipid profile at both low and high altitude

The aim of this study was to evaluate the exhaustive exercise induced stressful effect as well as parallel administration of vitamins E and C mediated protective effect on hepatorenal functions in rats native to low altitude (LA) and high altitude (HA). In both LA and HA areas, native wistar rats of each area were divided into three groups each of 6 rats, which include non-stress control, forced...

Author(s): Fahaid H. AL-Hashem, Abdullah Shatoor, Hussein F. Sakr,Nasser Al –Daghri, Mohammad Khalil and Mahmoud Alkhateeb

June 2012

Antimicrobial activity of seed, pomace and leaf extracts of sea buckthorn (Hippophae rhamnoides L.) against foodborne and food spoilage pathogens

The present study was conducted to evaluate the total phenolic content (TPC) and antibacterial properties of crude extracts of sea buckthorn (Hippophae rhamnoides L.) pomace, seeds and leaves against 17 foodborne pathogens. The methanolic extract of leaves exhibited high total phenolic content (278.80 mg GAE/g extract) and had low minimum inhibitory concentration (MIC) value of 125 µg/ml...

Author(s): Richa Arora, Sunil Mundra, Ashish Yadav, Ravi B. Srivastava and Tsering Stobdan

June 2012

Comparative study of biological activity of glutathione, sodium tungstate and glutathione-tungstate mixture

Glutathione (GSH) and sodium tungstate (Na2WO4) are important pharmacological agents. They provide protection to cells against cytotoxic agents and thus reduce their cytotoxicity. It was of interest to study the biological activity of these two pharmacological active agents. Different strains of bacteria were used and the zone of inhibition was determined for GSH, Na2WO4 and GSH+ Na2WO4 mixture. The results...

Author(s): Arshad Farid, Abdul Haleem Shah, Muhammad Ayaz, Adnan Amin, Muhammad Yaseen,  Hafeez Ullah and Fazal Haq

June 2012

An entropy-based improved k-top scoring pairs (TSP) method for classifying human cancers

Classification and prediction of different cancers based on gene-expression profiles are important for cancer diagnosis, cancer treatment and medication discovery. However, most data in the gene expression profile are not able to make a contribution to cancer classification and prediction. Hence, it is important to find the key genes that are relevant. An entropy-based improved k-top scoring pairs (TSP) (Ik-TSP)...

Author(s): Chunbao Zhou, Shuqin Wang, Enrico Blanzieri and Yanchun Liang

June 2012

Phenolic compounds and biological activity of Capsicum annuum L.

The objective of this study was to evaluate antifungal and antioxidant activities of vegetable extracts (Capsicum annuum L. cv. Dora, cv. Strizanka, cv. Morava), grown in Serbia. Different experimental models have included the determination content of total phenolics, total flavonoids, antioxidant capacity and minimum inhibitory concentration of the extract. The phenolic composition of different extracts was...

Author(s): Radoš Pavlovic,  Jelena Mladenovic, Blaga Radovanovic, Gordana Acamovic-Ðokovic, Jasmina Zdravkovic, Milan Zdravkovic

June 2012

Molecular and pathological identification of feline coronavirus type I

The coronavirus in cats has been described as feline infectious peritonitis (FIPV) and feline enteric coronavirus (FECV). FIPV is highly fatal and caused immune-mediated pyogranulomatous disease, whereas FECV causes mild enteric infection. In this study, we described the isolation and molecular characterization of naturally occurring feline coronavirus from domestic cat in Malaysia. Additionally, the resultant...

Author(s): Alazawy Amer, , Arshad Siti-Suri, Hair-Bejo Mohd, Omar Abdul-Rahman, Tengku-Ibrahim Tengku-Azmi, Bande Faruku and Assumaidaee Ajwad

June 2012

Soybean seed viability and changes of fatty acids content as affected by seed aging

  The characteristics of soybean seed chemical composition are related to specific processes occurring in seed during storage. These changes lead to seed aging during storage and affect seed vigour and content of fatty acids. In order to reveal severity of their influence, the following vigour tests were applied: Standard laboratory germination test, cold test, Hiltner test as well as accelerated aging test for...

Author(s): Mladen Tatić, Svetlana Balešević-Tubić, Vuk ĐorÄ‘ević, Zorica Nikolić, Vojin Đukić, Milka Vujaković and Gorica Cvijanovic