November 2014
Cellulase activity of filamentous fungi induced by rice husk
The objective of this study was to determine the potential of different filamentous fungi to degrade cellulose in rice husk pre-treated with steam explosion or alkaline hydrolysis. A preliminary test performed with carboxymethyl cellulose and nine fungi (Trichoderma 1, 2, 3, 4, 5; Trichoderma reesei; Aspergillus niger; Rhizopus oryzae and an isolated fungus from rice husk) allowed the selection of the fungi that can...
November 2014
Chitinolytic assay for Trichoderma species isolated from different geographical locations of Uttar Pradesh
Chitin is the most commonly available polymer on the earth. Cell walls of most of the fungi are made up of chitin. As we all know that Trichoderma produces a wide variety of cell wall degrading enzymes (CWDEs) such as chitinase, xylanase, glucanase and cellulase. Out of these CWDEs chitinase is of prime importance as it is the building block of fungal cell walls. For the detection of chitinase activity we used a simple...
November 2014
Comparative acute toxicity and oxidative stress responses in tadpoles of Amietophrynus regularis exposed to refined petroleum products, unused and spent engine oils
The relative acute toxicity of refined petroleum (diesel, kerosene and petrol), unused and spent engine oils as well as their abilities to alter the activities of superoxide dismutase (SOD) and cause lipid peroxidation in tadpoles of the common African toad, Amietophrynus regularis were evaluated. After 48 h of exposures, kerosene was found to be the most toxic (LC50= 4930 mg/L) while the least toxic was unused...
November 2014
Statistical optimization of lactic acid production by Lactococcus lactis strain, using the central composite experimental design
The individual and interactive effects of a total inoculums size (% v/v), fermentation temperature and skim milk dry matter added (% w/v) on the lactic acid production by Lactococcus lactis LCL strain were studied by quadratic response surface methodology. The central composite design (CCD) was employed to determine maximum lactic acid production at optimum values for process variables and a satisfactory fit model was...
November 2014
Somatic embryogenesis and plant regeneration from leaf explants of Rumex vesicarius L.
An attempt was made to study the somatic embryogenesis and plant regeneration from the in vitro leaf explants of Rumex vesicarius L. a renowned medicinal plant, which belongs to polygonaceae family. Effective in vitro regeneration of R. vesicarius was achieved via young leaf derived somatic embryo cultures. Embryogenic callus was induced from leaf explants on Schenk and Hildebrandt (SH) medium supplemented with various...
November 2014
Noble silver nanoparticles (AgNPs) synthesis and characterization of fig Ficus carica (fig) leaf extract and its antimicrobial effect against clinical isolates from corneal ulcer
Nanotechnology is rapidly growing with nanoparticles produced and utilized in a wide range of pharmaceutical and commercial products throughout the world. In this study, fig (Ficus carica) leaf extracts were used for ecofriendly extracellular synthesis of stable silver nanoparticles (AgNPs) by treating an aqueous silver nitrate (1 mM) solution and using the plant F. carica leaf extracts as reducing agents. The...
November 2014
A novel polymerase chain reaction (PCR) for rapid isolation of a new rbcS gene from Lemna minor
This study developed a novel polymerase chain reaction (PCR) method, ligation-mediated self-formed panhandle PCR, for gene or chromosome walking. It combined the advantages of ligation-mediated PCR in its specificity and of panhandle PCR in its efficiency. Self-formed panhandle PCR was used for a new rbcS gene walking to isolate 3’ downstream and 5’ upstream sequence; 1292 bp DNA rbcS gene was obtained via...
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