African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 6 December 2012; 11(98)

December 2012

Clinical and molecular genetics association of polymorphisms in interleukin-17A genes with risk of Oral Lichen Planus (OLP) in an Azery population

  Lichen Planus (LP) is a chronic disease that affects the skin and oral mucosa.Although the precise aetiology of LP is not known, it is thought to be comprised of, in majority, genetic and immunological factors. The objective of this study was to assess the association of interleukin-17A (IL-17A) with Oral LP (OLP). 22 adult patients with OLP and 40 healthy controls were genotyped by polymerase...

Author(s):   Firooz Puralibaba, Zahra Babalou, Alireza Sighary Deljavan, Farzaneh Pakdel, Masoud Sakhinia and Ebrahim Sakhinia

December 2012

Selection of an efficient in vitro micropropagation and regeneration system for potato (Solanum tuberosum L.) cultivar Desirée

  Sprouts of about 40 to 80 mm length were excised, surface sterilized with 70% Clorox® and cultured on solid full-strength Murashige and Skoog (MS) medium. Shoot nodal segments (1.0 cm) from in vitro plantlets (2 to 4 weeks old) were multiplied through periodic subculturing on full-strength MS medium with 30 g/L sucrose, 100 ml/L myo-inositol and 0.5 ml/L silver thiosulfate. The...

Author(s): Mahmoud M. Saker, Tarek A. A. Moussa, Nahed Z. Heikal, Amany H. A. Abo ELLiland Rehab M. H. Abdel-Rahman

December 2012

Population genetic study on common kilka (Clupeonella cultriventris Nordmann, 1840) in the Southwest Caspian Sea (Gilan province, Iran) using microsatellite markers

  This study represents population genetic analysis of the common kilka Clupeonella cultriventris (Nordmann, 1840) in the southwest Caspian Sea (Gilan province). A total of 60 specimens of adult common kilka were sampled from two seasons (spring and summer), 2010. Fifteen pairs of microsatellites previously developed for American shad (Alosa sapidissima), Pacific herring (Clupea pallasi), Atlantic...

Author(s): Mehrnoush Norouzi, Ali Nazemi and Mohammad Pourkazemi

December 2012

Development and optimization of hairy root culture systems in Withania somnifera (L.) Dunal for withaferin-A production

  Transformation of Withania somnifera was carried out by using three Agrobacterium rhizogenes strains (ATCC 15834, R1000 and K599) for hairy root induction. Induction of hairy root was carried out in leaf, petiole and internodal explants. Hairy root induction was successful only in ATCC 15834 and R1000. The highest frequency of hairy root was obtained in petiole explants (64%) infected with...

Author(s): Ananthapadmanaban Saravanakumar, Abubakker Aslam and Appakan Shajahan

December 2012

Inoculation of Ceratonia siliqua L. with native arbuscular mycorrhizal fungi mixture improves seedling establishment under greenhouse conditions

  The potential benefits of inoculation with arbuscular mycorrhizal (AM) fungi were investigated on carob tree Ceratonia siliqua, a Mediterranean legume in Morocco. The parameters under study were the effect of an inoculation on growth, mineral nutrition and roots mycorrhizal colonization of the plant under nursery conditions. C. siliquagrowth was measured after six months of culture in...

Author(s): Ouahmane Lahcen, Ndoye Ibrahima, Morino Abdessadek, Ferradous Abderrahim, Sfairi Youssef, Al Faddy Mohamed Najib and Abourouh Mohamed

December 2012

Assessment of genetic diversity in quality protein maize (QPM) lines using simple sequence repeat (SSR) markers

  Maize is a primary source of energy supplement and can contribute up to 30% protein, 60% energy and 90% starch in an animal’s diet. In the present investigation, 48 microsatellite markers, spread across the maize genome were used for analyzing genetic diversity among the sixty three quality protein maize (QPM) lines, including lines developed in India and CIMMYT, Mexico. Polymorphic profiles for...

Author(s): MSR Krishna, S. Sokka Reddy and V. Chinna Babu Naik

December 2012

Induction and flow cytometry identification of mixoploidy through colchicine treatment of Trigonella foenum-graecum L.

  Polyploidy plays an important role in plant evolution and constitutes an important mechanism of diversification and genetic variations creation. The objective of this study was to produce a polyploid cultivar of Trigonella foenum-graecum L. (2n=16) and to evaluate its cytological potentialities. Polyploidy induction was carried out by using a 0.05% colchicine solution. Ploidy level was determined by...

Author(s): Faten Omezzine, Afef Ladhari, Faten Nefzi, Rafik Harrath, Mahjoub Aouniand Rabiaa Haouala

December 2012

Spatial and temporal expression analysis of D-myo-inositol 3-phosphate synthase (MIPS) gene family in Glycine max

  Phytic acid, the principal storage form of phosphorus in plant seeds accounts for up to 60 to 80% of the total seed phosphorus content in soybean. Its accumulation increases linearly throughout seed development and it strongly chelates essential mineral cations and charged proteins decreasing their bioavailability. D-Myo-inositol 3-phosphate synthase (MIPS; EC, the evolutionarily conserved enzyme in...

Author(s): Swati Kumari, Monica Jolly, Veda Krishnan, Anil Dahuja and Archana Sachdev

December 2012

Effects of bonny light crude oil on anti-oxidative enzymes and total proteins in Wistar rats

  Crude oil (CO) is a substance trado-medically used within some rural population as an antidote to poisoning and a cure for various gastro-intestinal disturbances among others. The ingestion of crude oil either orally or through polluted marine species represents a pathway for the delivery of potential toxicants to the human system. The study, therefore, analysed the effects of bonny light crude oil on the...

Author(s): ODO Christian E., NWODO Okwesili F. C., JOSHUA Parker E., UBANI Chibuike S., ETIM Okon E. and UGWU Okechukwu P. C.