February 2013
Application of biologics to feedstuff
The application of biologics additives from enzyme, prebiotics and probiotics to improve the feedstuff utilization and animal growth is an aim for safety and hygiene animal products. These additives are gradually replacing the antibiotics. As biotechnology develops to meet animal demand, high bioavailability and low production cost technological products will be novel feed additives, including high production...
February 2013
Optimization of genetic transformation protocol mediated by biolistic method in some elite genotypes of wheat (Triticum aestivum L.)
We report here an efficient genotype-independent genetic transformation system in wheat. Highly regenerable embryogenic calli obtained from mature seeds were employed as the target tissue for the genetic transformation of three bread wheat varieties viz C306, HDR77 and PBW343 representing diverse genetic background. The plasmid pDM803 containing GUS and BAR genes driven by...
February 2013
Genetic variability, correlation and path analysis in sponge gourd (Luffa cylindrica Roem.)
The performance, genetic variability, heritability, genetic advance, correlation and path analysis for yield and yield contributing characters namely total yield per vine (kg), number of fruits per vine, average weight of fruit (g), average length of fruit (cm), average diameter of fruit (cm), days to anthesis of first male flower, days to anthesis of first female flower, node number at which first female...
February 2013
Marginal scorch caused by Alternaria alternata on Purple-Caitai (Brassia campestris L. ssp. chinensis L.var. utilis Tsen et Lee) in China
A new disease was detected in Purple-Caitai (Brassia campestris L. ssp. chinensisL.var. utilis Tsen et Lee) on the high mountain in Hubei, China. On the basis of morphological and cultural features, the pathogen was identified as Alternaria alternata. The internal transcribed spacer (ITS)-rDNA sequence of a representativeAlternaria isolate from Purple-Caitai showed 99% identity with...
February 2013
Effect of 1-methylcyclopropene on the antioxidant capacity and postharvest quality of tomato fruit
Tomato fruits ‘1402’ were harvested at mature green (MG), light pink (LP) and light red (LR) stages and treated with 0.3 µL L-1 of 1-methylcyclopropene (1-MCP) at 20°C for 24 h to investigate the ability to retard tomato fruit ripening. The treated and control fruit were stored at 5°C and 12 oC for 14 days and a further 4 days at 20oC for a shelf life period. The...
February 2013
Role of agricultural extension services in generation and dissemination of agricultural biotechnology in Abia State, Nigeria
Agricultural biotechnology has demonstrated that it can play a role in raising the level of food, feed and fibre production. However, for agricultural biotechnology to be effective and beneficial to the target end-user, agricultural extension services must be effective in achieving high rates of adoption. This study was undertaken to ascertain the role of extension services in generation and disseminating...
February 2013
Efficient regeneration of the endangered banana cultivar ‘Virupakshi’ (AAB) via embryogenic cell suspension from immature male flowers
Plantlets of the banana cultivar ‘Virupakshi’ (AAB) were regenerated from somatic embryos derived from embryogenic cells of calli from immature male flower explants. Induction of calli from explants was favored by a relatively moderate concentration of2,4-dichlorophenoxyacetic acid (2,4-D) (4 mg/L), high concentrations of proline and glutamine (both 300 mg/L) and coconut water....
February 2013
Potential of Laceyella sacchari strain B42 crude xylanase in biobleaching of kraft pulp
Xylanase producing thermophilic actinomycetes strain B42 was isolated from bagasse. This strain was enriched on oat spelt xylan agar medium and screened onto xylan-congo red agar plate by the xylanolysis method. The phylogenetic analysis using 16S rDNA sequence data confirmed that strain B42 showed highest homology (99.0%) with Laceyella sacchari and was identified as Laceyella...
February 2013
The effects of dietary supplementation of tomato peel ultrafine powder on glycemic response in streptozotocin-induced diabetic rats and blood lipids in high-fat diet rats
This study was designed to investigate the effects of dietary supplementation of tomato peel ultrafine powder (TPUP) on glycemic response in streptozotocin (STZ)-induced diabetic rats and blood lipids in high-fat diet rats. STZ-induced diabetic rats and high-fat diet rats were administrated orally with TPUP for 21 and 49 days and subjected to biochemical analysis of blood glucose...
February 2013
Effect of temperature and pH on ethanol production by a Blastomyces species isolated from the intestine of oil palm weevil (Rhynchophorus palmarum, coleoptera)
The effects of pH (4.5, 5.0, 5.5, 6.0, 6.5 and 7.0), temperature (20, 25, 30, 35, 40 and 45°C) and combinations of the two (pH 4.5, 20°C; pH 5.0, 25°C; pH 5.5, 30°C; pH 6.0, 35°C; pH 6.5, 40°C; pH 7.0, 45°C) on ethanol production by a putative Blastomycesspecies isolated from the intestine of oil palm weevil larva were studied. Peptone water was used as the growth medium and...
February 2013
Anti-inflammatory and anti-oxidant effects of Sterculia tragacantha fractions in mice
ix fractions obtained from the methanol leaf extract of Sterculia tragacantha were screened for anti-inflammatory and anti-oxidant activities. Treatment of mice with 10 and 90 mg/kg fractions 3, 4, 5, 6 and 7 significantly inhibited carrageenan induced paw edema in mice. The higher doses (90 mg/kg) showed superior anti-inflammatory activity compared to 10 mg/kg. The percentage edema...
February 2013
Effects of the methanol extract of Erythrina abyssinica on hot flashes in ovariectomized rats
We investigated the estrogen-like properties of the methanol extract of Erythrina abyssinica in ovariectomized rats. Climaterix was induced in female rats by surgically removing the ovaries. Data loggers implanted in the abdominal cavity during the procedure recorded core temperatures at predetermined time intervals for 72 h. 6 h after the onset of temperature recording, animals were treated with...
February 2013
Xanthomonas campestris pv. campestris race 1 is the main causal agent of black rot of Brassicas in Southern Mozambique
Severe outbreaks of bacterial black rot caused by Xanthomonas campestris pv.campestris (Xcc) were observed in Brassica production fields of Southern Mozambique. The causal agent of the disease in the Mahotas and Chòkwé districts was identified and characterised. In total, 83 Xanthomonas-like strains were isolated from seed samples and leaves of cabbage and...
February 2013
Morphological development of the small intestine in white Roman goslings
The objective of this study was to investigate the morphological development of small intestinal segments in white Roman goslings from hatching to 28 days of age. Forty day-old male goslings were used in this experiment. Eight goslings were selected randomly at hatching, 7, 14, 21 and 28 days of age, respectively. The small intestinal segments were sampled from six goslings for intestinal structure...
February 2013
Serum biochemical and liver enzymes changes in dogs with single and conjunct experimental infections of Trypanosoma brucei and Ancylostoma caninum
The serum biochemical changes that occur in dogs with single and conjunct experimental infections of Trypanosoma brucei and Ancylostoma caninum were studied. Four groups (GPI, GPII, GPIII and GPIV) of five dogs each were used for this study. GPI was the uninfected control while GPII, GPIII and GPIV were infected with A. caninum, T. brucei and conjunct A. caninum/T....
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