African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Table of Content: 7 August 2013; 12(32)

August 2013

Characterization of Tomato Leaf Curl New Delhi Virus infecting cucurbits: Evidence for sap transmission in a host specific manner

  Sponge gourd (Luffa cylindrica) is an economically important vegetable crop cultivated throughout  India and this crop is severely affected by yellow mosaic disease caused by begomovirus. In this study, an attempt was made to transmit the begomovirus by sap. The causal agent was easily transmitted by sap to ridge gourd, sponge gourd andNicotiana benthamiana. Several factors affecting the efficient...

Author(s):   Sayed Sartaj Sohrab, Sajjad Karim, Anupam Varma, Adel Muhammad Abuzenadah, Adeel Gulzar Chaudhary, Ghazi A Damanhouri and Bikash Mandal    

August 2013

A critical scientific review on South African governance of genetically modified organisms (GMOs)

  Credible governance of genetically modified organisms (GMOs) is essential because of public concerns in South Africa (SA) and internationally. In this preliminary study, the opinions of a number of scientists with experience and/or interest in GMO governance were determined by means of two questionnaires to determine their perceptions on the credibility of risk governance of GMOs in SA.  The respondents...

Author(s):   F. W. Jansen van Rijssen, E. Jane Morris and J. N. Eloff        

August 2013

Genetic analysis of japonica x indica recombinant inbred lines and characterization of major fragrance gene by microsatellite markers

  Traditional basmati rice varieties are very low yielding due to their tendency to lodging and increasing susceptibility to diseases. To improve the characters of basmati rice variety and study the inheritance of various physio-morphological and quality characters, F5 population comprising of 204 lines from the cross between NPT II (non-aromatic, japonica) and Taraori Basmati or HBC19...

Author(s):   Vikram Singh, R. K. Jain and Mukesh Kumar        

August 2013

Rapid approach for cloning bacterial single-genes directly from soils

  Obtaining functional genes of bacteria from environmental samples usually depends on library-based approach which is not favored as its large amount of work with small possibility of positive clones. A kind of bacterial single-gene encoding glutamine synthetase (GS) was selected as example to detect the efficiency of cloning strategy in this study. Five GS genes were directly cloned from soils using...

Author(s):   Chenguang Zhu, Peipei Wang, Chunlei Shen, Wei Wang, Yuanping Tang, Bing Mei, Zhengkai Xu and Rentao Song        

August 2013

Genetic differentiation between the black skinned and white skinned snails (Archachatina marginata) using random amplified polymorphic DNAs

  The study investigates the genetic differentiation between the black skinned and white skinned ectotypes of the giant African land snails (Archachatina marginata) from Cross River State in Niger Delta region of Nigeria. The random amplified polymorphic DNA (RAPD) technique was employed in this study. Five (5) oligonucleotide primers (OPAD-09, OPAE-04, OPAE-05, OPAF-07 and OPAF-09) were used to...

Author(s):   Okon, B., Ibom, L. A. and Njume, G. N.        

August 2013

Molecular study on extended spectrum β-lactamase-producing Gram negative bacteria isolated from Ahmadi Hospital in Kuwait

    During the period from November 2009 to April 2010, 84 out of 560 extended spectrum β-lactamase (ESBL) producing negative bacteria were isolated from patients in different departments of the Ahmadi Hospital in Kuwait. The isolates were collected from urine catheter, wound, sputum, blood and other different samples. The ESBL infection rate in the in-patients was 62% and part of them (19%) were...

Author(s):   Eshaq A. Mohmid, El-Sayed A. El-Sayed and Mahmoud F. Abdel El-Haliem        

August 2013

A PCR ITS-RFLP method for identifying fungal contamination of date palm (Phoenix dactylifera L.) tissue cultures

  Morphological and molecular procedures have been used in the study to characterize the most abundant fungal contaminants of date palm tissue cultures. Aspergillusspecies were found to be the most frequently isolated species, followed by the species of Alternaria and Penicillium. Internal Transcribed Spacer (ITS) products of the predominant fungi were analyzed by PCR amplification with ITS1...

Author(s):   Mohammed H. Abass        

August 2013

Biosorption of cadmium (ii) ion from aqueous solution by Afzelia africana

  The batch adsorption of cadmium (II) ion from aqueous solution using low-cost adsorbent of biological origin, Afzelia africana shell under different experimental conditions was investigated in this study. The influences of initial Cd (II) ion concentration, initial pH, contact times and temperature were reported. Adsorption of Cd (II) was found to be pH dependent and the results indicate that the...

Author(s):   Onwu, F. K., Ogah, S. P. I. and Ngele, S. O.      

August 2013

Remediation of lead (Pb) by a novel Klebsiella sp. isolated from tannery effluent of Ranipet, Vellore district

  Lead is found to be one of the most toxic heavy metal according to American public health association (APHA). Vellore district is one of the most polluted sites in the world. It is more common for lead poisoning to build up slowly over time. Over time, even low levels of lead exposure can harm a child's mental development. Therefore new resources for the removal of lead are the need of the hour. Soil and...

Author(s):   Anish Saini, Rohini Kumar M., Karthik Senan, Shakti Sagar, Vivekanandan K. E. and Jabez Osborne W.        

August 2013

Effect of ethanol seed extract of Buccholzia coriacea (wonderful kola) on the lipid profile of albino rats

  This study investigates the effect of ethanol extract of Buccholzia coriacea seeds on the serum lipid profile of albino rats within a period of two weeks of treatments. Twelve (12) Wistar albino rats weighing between 85 to 130 g purchased and randomly divided into three groups were used for this study. The control group received normal saline while the two other groups received graded...

Author(s):   Enechi, O. C., Manyawo, Lewis, N. and Ugwu Okechukwu P. C.        

August 2013

Anti-ulcer activity of aqueous leaf extract of Nauclea latifolia (rubiaceae) on indomethacin-induced gastric ulcer in rats

  Nauclea latifolia is known to possess various therapeutic properties. The present study was designed to evaluate the anti-ulcer activity of aqueous leaf extract of N.latifolia against indomethacin-induced gastric ulcers in rats. Five groups of albino rats were pre-treated orally with: vehicle, distilled water (ulcer control), cimetidine (100 mg/kg, reference control), and 170, 340 and 510...

Author(s):   Balogun M. E., Oji J.O., Besong E. E., Ajah A. A. and Michael E. M.      

August 2013

Evaluation of physicochemical, functional and textural properties of rainbow trout (Oncorhynchus mykiss) stored at low temperatures

  The physicochemical and functional behaviour of rainbow trout stored at 4°C and -20°C for 9 days and 6 months, respectively, were investigated. The combined effect of proteolytic activity and physicochemical changes in the muscle (pH, solubility and proteolytic activity) altered the molecular structure of the proteins. SDS-PAGE showed the degradation of myosin and the formation of peptides of low...

Author(s):   Arturo Silva-Ríos, Octavio Dublán-García, Baciliza Quintero-Salazar, Aurelio Dominguez-Lopez, Leobardo Manuel Gómez-Oliván, Leticia Xochitl López-Martínez and Abdel-Fattah Z. M. Salem,    

August 2013

Comparative study of intestine length, weight and digestibility on different body weight chickens

  This experiment was conducted to compare the difference of digestibility on different body weight chickens. Twenty-seven (27) 58-week-old New Yangzhou Chickens of three grade sizes (small 2.0 kg, medium 2.5 kg, and large 3.0 kg) were selected and distributed into three groups (Group 1, Group 2, Group 3) of nine birds/group, and each group was represented by three replicates. Nutrient retention ratio was...

Author(s):   H. M. Yang, W. Wang, Z. Y. Wang, J. Wang , Y. J. Cao and Y. H. Chen        

August 2013

Xanthine oxidase activity during transition period and its association with occurrence of postpartum infections in Murrah buffalo (Bubalus bubalis)

  The aim of this study was to quantify xanthine oxidase (XO) levels during the transition period in Murrah buffalo (Bubalus bubalis) and determine its association with certain postpartum infections. For this, six healthy buffaloes were selected from the National Dairy Research Institute (NDRI) herd and managed under standard managemental practices as followed at the institute. Blood samples were drawn weekly...

Author(s):   Bhabesh Mili, Sujata Pandita, Bharath kumar B.S., Anil Kumar Singh, Madhu Mohini and Manju Ashutosh        

August 2013

Bioregulators as tools for plant growth, development, defence and improvement

Bioregulators are chemicals that affect the expression of biological responses in plant tissues. Their use is a unique facet of biotechnology and a new approach of manipulating plant biochemistry for enhancing productivity and quality. They act in low concentrations without any biocidal or nutritive action. Applications of bioregulators are reported to reduce biotic and abiotic stress in plants. Recent studies...

Author(s): Olaiya, C. O., Gbadegesin, M. A. and Nwauzoma, A. B.