October 2014
Molecular investigation of two contrasting genotypes of Medicago truncatula to salt stress using two expressed sequence tag-simple sequence repeat (EST-SSRs) markers
Two expressed sequence EST-SSRs primers were used to show genetic variation and determine a potential link of these markers to salt stress tolerance on two contrasting Medicago truncatula genotypes (Tru 131 tolerant genotype, and Jemalong, sensitive one). The amplification of the DNA were isolated from 10 individual seedlings for each genotype (tolerant and sensitive) with two Expressed Sequence Tag-Simple Sequence...
October 2014
Targeted parallel sequencing of the Musa species: Searching for an alternative model system for polyploidy studies
Modern day genomics holds the promise of solving the complexities of basic plant sciences, and of catalyzing practical advances in plant breeding. While contiguous, "base perfect" deep sequencing is a key module of any genome project; recent advances in parallel next generation sequencing (NGS) technologies has opened up new avenues for answering biological questions in moderate to large genomes of complex...
October 2014
In vitro multiple shoot bud induction and regeneration from plumule junction explants of pigeon pea [Cajanus cajan (L.) Mill sp.] cultivars
The response of eleven Indian cultivars of pigeon pea for in vitro multiple shoot bud induction and regeneration from plumule junction explants under variable concentration of 6-benzyl amino purine (BAP), kinetin and thiadiazuron (TDZ) was assessed in the present study. The cultivar IPA-3088 showed best response with a maximum of 20 buds per explants in Murashige and Skoog (MS) media supplemented with 0.05 mgL-1 TDZ....
October 2014
Control of root-knot nematode by using composted sawdust in tomato root
The effect of composted sawdust at different concentrations (0, 10, 20... 100% v/v in soil) on tomato plant growth and pigments were investigated with or without the presence of root-knot nematode, Meloidogyne javanica at different inoculum levels (0, 1000, 2000, 3000, 4000, 5000) in clay pots (having diameter 30 cm and depth 60 cm). An increase in the composted sawdust concentration in the field soil progressively...
October 2014
Interaction between faba bean cultivars and the Rhizobium leguminosarum strains: Symbiotic N2 fixation and protein profiles under salt stress
The efficiency of eight Rhizobium leguminosarum strains isolated from root nodules of Faba bean plants which were collected from different Governorate in Egypt has been examined. After inoculation of faba bean seeds with each Rhizobium strain individually, we measured the growth promotion, nodule formation and plant protein profile under different concentration of sodium chloride salt stress. The interactions between...
October 2014
Effect of UV-C irradiation on antioxidant activities, total phenolic and flavonoid contents and quantitative determination of bioactive components of Moringa oleifera Lam. shoot culture
Effect of UV-C irradiation on the antioxidant activities of shoot cultures of Moringa oleifeira Lam. was investigated. Total phenolic and flavonoid contents and the antioxidant bioactive components were determined. The shoots of M. oleifeira were cultured for 6 weeks on Murashige and Skoog (MS) mediums containing 0.5 mg/L 6- benzyladenine (BA) for multiple shoot formation. Multiple shoots were treated with UV-C...
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