January 2013
DNA-based population density estimation of black bear at northern Mexico: A preliminary study
The analysis of deoxyribonucleic acid (DNA) microsatellites from hair samples obtained by the non-invasive method of traps was used to estimate the population density of black bears (Ursus americanus eremicus) in a mountain located at the county of Lampazos, Nuevo Leon, Mexico. The genotyping of bears was performed by multiplex polymerase chain reaction (PCR) using an average of two...
January 2013
Molecular and morphological characterization of Phyllactinia cassiae-fistulae (Erysiphaceae; Ascomycota) from Thailand
Phyllactinia cassiae-fistulae and its Ovulariopsis anamorph, a causal agent of powdery mildew on Cassia fistula, have been found in Thailand for the first time. Phylogenetic analysis using the 28S ribosomal DNA sequences clearly demonstrated that P. cassiae-fistulae distinctly formed a unique clade at the basal part ofPhyllactinia with 100% bootstrap support. This...
January 2013
Short-duration exposure to 2.45 GHz microwave radiation induces DNA damage in Sprague Dawley rat’s reproductive systems
The genotoxic effects of 2.45 GHz microwave (MW) radiation on the testis and ovary of Sprague Dawley rats was investigated. The animals were exposed to varying levels of specific absorption rate (SAR) of 0 (control), 0.48, 0.95, 1.43, 1.91, 2.39, 2.90, 3.40, 3.80 and 4.30 Wkg-1, for 10 min. The induction of DNA damages was assessed using DNA direct amplification of length polymorphisms (DALP) and...
January 2013
Genetic diversity in South African maize (Zea mays L.) genotypes as determined with microsatellite markers
One thousand and forty three (1043) maize genotypes including white and yellow maize inbred lines as well as hybrids from the public germplasm collection were characterized with 80 microsatellite markers distributed throughout the genome. A total of 1874 alleles were amplified and used in the genetic diversity analysis. Principal coordinate analysis confirmed the geographical distribution of the breeding...
January 2013
Genotype identification and inference of genetic relatedness among different purpose grape varieties and rootstocks using microsatellite markers
Twenty-seven (27) grape genotypes representing different grape groups namely table, wine, juice grapes and rootstocks with present or potential future importance for sub-tropical viticulture were characterized with 29 microsatellite markers spanning over 15 linkage groups of ‘Syrah’ ´ ‘Grenache’ linkage map. The objective of the investigation was to establish the unique...
January 2013
Seasonal variation in the anti-nutrient and mineral components of some forage legumes and grasses
This study was conducted in Nsukka, Nigeria to evaluate the effect of season of harvest (rain and dry) on the anti-nutrient and mineral components of four herbaceous legumes (Calapogonium mucunoides, Centrosema pubescens, Stylosanthesguyanensis and Pueraria phaseoloides) and four forage grasses...
January 2013
Growth and photosynthesis responses of Rosmarinus officinalis L. to heavy metals at Bougrine mine
Heavy metals pollution is considered as one of the most dangerous environmental problems in agricultural soil, particularly neighboring mining sites. Phytoremediation based on green plants use seems to be an interesting biological solution to detoxify these soils. This study focuses especially on heavy metals effects on Rosmarinus officinalis L. morphological and growth parameters. The site of our...
January 2013
Asymbiotic germination of immature embryos of a medicinally important epiphytic orchid Acampe papillosa (Lindl.) Lindl.
The immature embryos (28 weeks after pollination) were inoculated on M (Mitra et al., 1976), and PDA (Potato Dextrose Agar) media, with and without different growth additives. The seeds showed positive germination response in both the nutrient media but the frequency and onset of germination response and associated morphogenetic changes leading to seedling development varied with the nature of growth...
January 2013
Enhancing blooming period and propagation coefficient of tulip (Tulipa gesneriana L.) using growth regulators
A study was carried out to evaluate the effect of plant growth regulators on growth, flowering and bulb production of tulip under Karewa conditions of Kashmir Himalaya during 2009 to 2011. The three different growth regulators; gibberellic acid (GA3) at 100, 200, and 400 ppm, 2-chloroethyl trimethyl ammonium chloride (CCC) and maleic hydrazide (MH) each at 100, 200 and 500 ppm along with control were applied...
January 2013
Antagonistic effect of brevicin on Gram positive and Gram negative food borne bacteria and its biopreservative efficacy in milk
A new low molecular weight brevicin produced by Lactobacillus brevis NS01 has greater antimicrobial activity on Gram positive and negative food borne bacteria. This is stable at high temperature acidic to neutral pH, non proteolytic enzymes and organic solvents. The synergistic effect of brevicin with ethylenediaminetetraacetic acid (EDTA), sodium lactate (NaL) and potassium sorbate...
January 2013
Vitamin C stability in pineapple, guava and baobab juices under different storage conditions using different levels of sodium benzoate and metabisulphite
Vitamin C is one of the most important vitamins in fruit based products and its stability is very critical to guarantee its availability. Stability of vitamin C in baobab, guava and pineapple juices stored at room (22.4°C±1.3), chilling (-1.2°C±0.1) and freezing (-17.3°C±0.2) temperatures and using 0.05% sodium benzoate, 0.005% sodium metabisulphite and combined use of...
January 2013
Microbiological, physico-chemical and sensory quality aspects of yoghurt enriched with Rosmarinus officinalis oil
Two types of yoghurt with Rosmarinus officinalis oil was prepared and stored up to 21 days. The extract plant was added at the rate of 0.14, 0.21, 0.29, and 0.36 g/L. Yoghurt samples were analyzed for some physical, chemical, microbiological and sensory some characteristics. The total and fecal coliform count, Staphylococcus aureus count, Salmonella count, yeast and...
January 2013
Mead features fermented by Saccharomyces cerevisiae (lalvin k1-1116)
Alcoholic beverages are produced practically in every country in the world representing a significant percentage of the economy. Mead is one of the oldest beverages and it is easily obtained by the fermentation of a mixture of honey and water. However, it is still less studied compared to other beverages and does not have industrialized production. It is prepared as a handmade product. The origin of...
January 2013
In vitro comparative effect of extracts of the seeds of Embelia rowlandii (Myrsinaceae) on the eggs and L1 and L2 larval stages of the parasitic nematode Heligmosomoides bakeri (Nematoda; Heligmosomatidae)
In vitro ovicidal and larvicidal activities of extracts of the seeds of Embelia rowlandiiwere investigated on unembryonated and embryonated eggs, first and second stage larvae of Heligmosomoides bakeri. Three different extracts were prepared with distilled water (DW) and 95% ethanol. The organic extract was first diluted with 3% Tween and then distilled water added to obtain five different...
January 2013
Analgesic and anti-inflammatory effects of Crinum asiaticum leaf alcoholic extract in animal models
This study investigated the analgesic and anti-inflammatory effects of Crinum asiaticum (Amaryllidaceae) leaf ethanolic extract. Analgesic effect was investigated in acetic acid induced writhing model and formalin induced licking model in swiss albino mice. Anti-inflammatory effect was conducted in carrageenan-induced paw edema model...
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