July 2014
Biogenesis of nanoparticles: A review
Advancement in nanotechnology mainly depends upon advancement in nanomaterial. There are many chemical routes known to use toxic chemicals for synthesis of nanoparticles but the need of the hour is to use environmental benign, greener and safer routes. Researchers are looking to use various living organisms as ‘nanoparticle factories’. Various biological entities like bacteria, fungi, diatoms, higher plants,...
July 2014
Genetic diversity and conservation of Picea chihuahuana MartÃnez: A review
The conservation of genetic diversity in tree populations is an essential component of sustainable forest management. Picea chihuahuana Martínez is an endemic conifer species in Mexico and is considered to be endangered. P. chihuahuana covers a total area of no more than 300 ha at the Sierra Madre Occidental, a mountain range that harbor a high diversity of tree species. There are 40 populations of the species...
July 2014
Appraisal of biochemical and genetic diversity of mango cultivars using molecular markers
Mango (Mangifera indica L.) is one of the oldest fruit crops and is broadly cultivated worldwide. To determine the level of genetic diversity, a total of 13 mango genotypes have been collected from different farms of Fayoum oasis in Egypt and were analyzed using molecular (DNA) and biochemical (SDS-PAGE) markers along with the quantification of soluble carbohydrates, chlorophyll and carotenoids. These profiles were...
July 2014
Segregation of vegetative and reproductive traits associated with tuber yield and quality in water yam (Dioscorea alata L.)
Water yam (Dioscorea alata L.) is an important source of food for millions of people in Africa, Asia, South America, Caribbean and the South Pacific islands. Genetic mapping populations of this species have been produced as part of efforts to raise the efficiency of breeding through development and use of molecular markers. In this study, an F1 mapping population (AM1) of D. alata, exhibited segregation for both tuber...
July 2014
Can hydrogen peroxide and quercetin improve production of Eucalyptus grandis x Eucalyptus urophylla?
Vegetative propagation is considered the best choice for the rapid multiplication of plant species, however, rooting may still present difficulties. Substances, such as auxins, phenolic compounds and hydrogen peroxide, are recognized as able to improve this process. The aim of the present work was to determine if hydrogen peroxide in combination with quercetin or indole butyric acid, can modify some characteristics...
July 2014
Effects of plant growth regulators on in vitro cultured nodal explants of cassava (Manihot esculenta Crantz) clones
Cassava (Manihot esculenta Crantz) is one of the major food security crops in Ethiopia. Recently, clean planting materials of improved cassava cultivars are in high demand. A limitation, however, is the low multiplication ratio (1:10) of the crop via conventional methods. Thus, a study was undertaken to develop an efficient in vitro mass propagation protocol for two elite cassava clones, 44/72-NR and 44/72-NW....
July 2014
Involvement of a hypersensitive-like reaction in tolerance to fire blight in pear (Pyrus communis L.)
Infection by Erwinia amylovora, the causal agent of fire blight (FB) disease, induces in apple and pear host plants, the generation of reactive oxygen species (ROS). We analyzed at molecular level, the link between ROS production and susceptibility to bacterial infection. Gene expression time course showed that expression of chloroplast, mitochondrial and nuclear genes, whose transcription is redox-dependent, was down...
July 2014
Influence of 24- epibrassinolide on in vitro shootlets regeneration via direct organogenesis of Phaseolus vulgaris L.
This study aimed to optimize an in vitro shootlets regeneration system via direct organogenesis for Phaseolus vulgaris L. cv. Brunca using 24- epibrassinolide (24-Epi). The best medium for shootlets proliferation as well as growth parameters was MS supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi. Stem explants recorded the best results of shootlets proliferation percentage compared with leaf or root explants,...
July 2014
Production of extracellular lipase by a new strain Staphylococcus aureus NK-LB37 isolated from oil contaminated soil
A total of 20 bacterial isolates were obtained by screening using tributyrin agar medium. Among them the isolate which exhibited greater clearance zone and higher lipase activity was subsequently screened using spirit blue agar and rhodamine B agar medium. Based on morphological, biochemical and 16S rRNA sequence analysis, the potent isolate was identified as Staphylococcus aureus. The lipase production of the isolate...
July 2014
Amino acids analysis during lactic acid fermentation by single strain cultures of lactobacilli and mixed culture starter made from them
The interactions among Lactobacillus salivarius, Pediococcus acidilactici and Lactobacillus plantarum in utilizing the amino acids of MRS media were investigated. L. salivarius alone showed relatively good assimilation of various amino acids that existed at only a little amounts in MRS media (Asn, Asp, Cit, Cys, Glu, His, Lys, Orn, Phe, Pro, Tyr, Arg, Ile, Leu, Met, Ser, Thr, Trp and Val), whereas Ala and Gly...
July 2014
Production of ethanol from tuberous plant (sweet potato) using Saccharomyces cerevisiae MTCC-170
The aim of this work was to research a bioprocess for bioethanol production at laboratory scale from raw sweet potato using Saccharomyces cerevisiae MTCC-170. In order to obtain maximum conversion of starch into fermentable sugar, optimum parameters for the liquefaction were determined as 104 to 105°C, 0.15% v/w of α-amylase enzyme solution (300 U/ml) and 30 g dry-weight sweet potato mash/100 ml distilled...
July 2014
Immuno-histochemical localization of cholesterol binding proteins in Schistocerca gregaria (Forskal)
This manuscript aims to investigate immunocytochemical localization of cholesterol binding proteins (CBPs) in semi-thin sections of midgut of Schistocerca gregaria (Forskal). For this purpose, polyclonal antibody specific to CBPs were raised in albino mice and used in immuno-fluorescence and immuno-blotting to determine the cellular location of CBPs. Midgut tissue sections were incubated with pAbs anti-cholesterol...
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