August 2014

Plasmodium berghei ANKA: Selection of pyronaridine resistance in mouse model

Pyronaridine is a partner drug in, Pyramax®, a combination of artesunate (ASN)-pyronaridine (PRD) which was recently prequalified by WHO drug as a potential alternative for treatment of malaria in African setting. Pyronaridine is a mannich base, with a long half-life, thus predisposed to resistance. In this study, we selected pyronaridine resistance by submitting Plasmodium berghei ANKA line in vivo to increasing...

Author(s): Kimani S. K., Ng’ang’a J. K., Kariuki D. W., Kinyua J., Kimani F. T. and Kiboi D. M.,

August 2014

Influence of CaCl2 and EDTA on reversible thermal inactivation of recombinant wild-type and mutant (E40H/E44H) Phlebia radiata manganese peroxidase 3 (rPr-MnP3)

Thermal inactivation of a new recombinant Phlebia radiata manganese peroxidase (rPr-MnP3) in the presence and absence of additives (CaCl2 and EDTA) is described for the first time. The influence of temperature and melting points (Tm) on the stability of rPr-MnP3 and its mutant (E40H/E44H) were determined. There was no significant inactivation at 25 – 40°C. However, we observed rapid inactivation of rPr-MnP3 at...

Author(s): Usenobong F. Ufot and Monday I. Akpanabiatu