African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Bacillus mucilaginosus can capture atmospheric CO2 by carbonic anhydrase

Zhicai Zhang1,2, Bin Lian1*, Weiguo Hou1, Mingxia Chen2, Xin Li2 and Yan Li3
  1State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, Guizhou 550002, P. R. China. 2School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P. R. China. 3School of Foreign Language, Jiangsu University, Zhenjiang 212013, P. R. China
Email: [email protected]

  •  Accepted: 29 December 2010
  •  Published: 18 January 2011



The study mainly demonstrated whether Bacillus mucilaginosus can use atmospheric carbon dioxide (CO2) through the bacteria excreted carbonic anhydrase (CA) capturing atmospheric CO2. It had been proved that CA could reversibly catalyze hydration of CO2. When the bacteria was cultured in the medium containing limestone, Ca2+ concentration and CA activities simultaneously increased with the amount of limestone increasing in the medium. Analysis for the bacterial growth kinetics demonstrated Ca2+ concentration increased, CA activities enhanced and pH value of broth rose and residual glycerol concentration decreased with prolonging fermentation time. Ca2+ concentration of bacterial broth gradually increased with the increasing of amount CA supply and time extension when the bacteria were cultured in the Alexandrov medium containing limestone, in which CA synthesized was extremely low when limestone dissolved into water, CA could elevate Ca2+ concentration and pH value, and the bacteria might further elevated the Ca2+ concentration and pH value. Therefore, it is suggested that B. mucilaginosus can first capture atmospheric CO2 by CA, then fix atmospheric CO2 by bacteria metabolism, which complements the content of biology and provide a new strategy for CO2 sequestration from the air.


Key words: Bacillus mucilaginosus, carbonic anhydrase equilibrium point, CO2 utilization,