African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Comparison of different detoxification methods for corn cob hemicelluose hydrolysate to improve ethanol production by Candida shehatae ACCC 20335

  Jing-Ping Ge, Bai-Yan Cai, Guo-Ming Liu, Hong-Zhi Ling, Bao-Zhu Fang, Gang Song, Xiao-Feng Yang and Wen-Xiang Ping*
No 74, Nangang District, Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Haerbin, 150080 P. R. China,
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 18 May 2011



Corn cob hydrolysis with 1% (v/v) of sulfuric acid yielded 36.49 total reducing sugars (g/l) along with various fermentation inhibitors such as fufural, phenolics compounds and acetic acid. The acid hydrolysate detoxified with overliming plus activated charcoal brought about a maximum decrease in fufural (100%), acetic acid (62.4%) and phenolics compounds (96.6%). Treatment of hydrolysate with overliming caused 99, 51.4 and 16.7% loss in fufural, acetic acid and phenolics compounds, respectively. Fermentation byCandida shehatae ACCC 20335 showed a maximum yield of ethanol (0.31 g/g) and a productivity of 0.152 g/l/h with overliming plus activated charcoal treated hydrolysate.


Key words: Corn cob, detoxification, ethanol, fermentation, hydrolysate.