African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Evaluation of the correlation between Aflatoxin B1 production and Aspergillus contamination in rice bran from northern Iran

Fatemeh Zaboli1*, Alireza Khosravi2 and Hojjatollah Shokri3
  1Science and Research Branch, Islamic Azad University (IAU) of Tehran, Iran. 2Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. 3Faculty of Veterinary Medicine, University of Mazandaran, Amol, Iran.
Email: [email protected]

  •  Accepted: 10 May 2011
  •  Published: 04 June 2011

Abstract

 

Aflatoxins are potent mycotoxins, which are produced by toxigenic Aspergillus species including Aspergillus flavus and Aspergillus parasiticus. The goals of this study were to characterize Aspergillus fungus isolated from rice bran and to determine the correlation between Aflatoxin B1 (AFB1) production and Aspergillus contamination in the samples. Thirty rice bran samples were collected from different regions of Mazandaran province, northern Iran. Samples containing 15 specimens were conserved for one year in the storage and the other one was not subjected to storage. Mycological analysis of rice bran was performed and AFB1 was purified from the samples and quantified by high performance liquid chromatography (HPLC) method with fluorimetry detection. The results of this study showed that almost all collected samples contained AFB1. The averages of AFB1 in new and old rice bran samples were found to be 5.07 and 6.81 µg/kg, respectively. No significant difference was obtained between AFBvalue in new and old samples. In addition, the correlation between culture results and aflatoxin production were significantly observed only in old samples (P<0.05). The most frequent isolatedAspergillus species were Aspergillus terreus, A. flavus, Aspergillus niger, A. parasiticusand Aspergillus fumigatus. In conclusion, these results confirmed that rice bran could be contaminated with AFB1 and toxigenic Aspergillus species and human beings are in danger of been affected by contaminated rice products.

 

Key words: Aflatoxin B1, rice bran, toxigenic Aspergillus species.