African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

In vitro assessment of Inula spp. organic extracts for their antifungal activity against some pathogenic and antagonistic fungi

  Faten Omezzine1*, Mejda Daami-Remadi3, Asma Rinez1, Afef Ladhari1 and Rabiaa Haouala2
  1Department of Biology, Faculty of Sciences of Bizerte, University of Carthage, Amilcar 1054, Tunisia. 2Department of Biological Sciences and Plant Protection, Higher Agronomic Institute of Chott Meriem, University of Sousse, Chott Meriem 4042, Tunisia. 3Laboratory of Plant Pathology, Regional Center of Research in Horticulture and Organic Agriculture, University of Sousse, Chott Meriem 4042, Tunisia.
Email: [email protected]

  •  Accepted: 16 September 2011
  •  Published: 09 October 2011



Inula viscosa, Inula graveolens and Inula crithmoïdes (Asteraceae) leaf and flower organic extracts (hexane, chloroform and methanol) were assessed for their antifungal activity against two Trichoderma species (Trichoderma harzianum and Trichoderma viride) and three formae speciales of Fusarium oxysporumI. viscosa organic extracts showed an important inhibitory activity against all target fungal isolates. Growth inhibition percentage ranged between 17-61, 77-100, and 55-100% in presence of hexane, chloroform and methanol I. viscosa leaf extracts, respectively. Flower organic extracts reduced mycelial growth of all fungi by 32-66, 30-75, and 8-70%, respectively. For I. graveolens, Stem + leaf organic extracts showed more or less important inhibition depending on solvent nature, though, flower organic extracts were found to be the most effective against tested fungi: a total inhibition of growth was recorded with methanol fraction against all target fungi and with hexane fraction against F. oxysporum f. sp. melonis (FOM) and T. viride. In presence of I. crithmoïdes leaf organic extracts, a total growth inhibition was noted with the three extracts against T. harzianum, with hexane and methanol fraction against T. viride and with chloroform fraction against FOM. I. crithmoïdes flower extracts caused a highly significant growth inhibition of about 57-100, 66-100, and 100-100% with hexane, chloroform and methanol extracts, respectively. Therefore, I. viscosa, I. graveolens and I. crithmoïdes could be an important source of biologically active compounds useful for developing environmentally safe antifungal products.


Key words: Antifungal activity, organic extracts, Inula, radial growth.