African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Isolation and characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing plant growth-promoting rhizobacteria from carnation soil and roots

Haiquan Huang1,3*#, Meijuan Huang1*#, Guosheng Fan1*, Xuefei Liu1, Jihua Wang2, Qing Duan2 and Qixiang Zhang3*
1School of Landscape Architecture, Southwest Forestry University, Kunming 650224, China. 2Institute of Flower Research, Yunnan Provincial Academy of Agricultural Sciences, Kunming 650205 China. 3School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China.
Email: [email protected]; 27495555[email protected]; [email protected]; [email protected].

  •  Accepted: 08 February 2013
  •  Published: 31 December 2013


Five strains of plant growth-promoting rhizobacteria (PGPR) with 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity were isolated from carnation soil and roots using ACC as the sole nitrogen source. Based on their growth morphological, microscopic cell properties and 16S rRNA sequence analysis, the results showed that three strains were identified as Enterobacter section and one as Erwinia among four strains from the soil, and one from carnation roots wAS identified as Acinetobacter; there are some differences in ACC deaminase activities among all isolated strains in this study. It is suggested that ACC deaminase-containing PGPR could be a cost-effective, environment-friendly and promising potential strategy to promote plant growth, alleviate biotic and abiotic stresses and ensure sustainable agriculture, especially for ethylene-sensitive flowers production.


Key words: 1-Aminocyclopropane-1-carboxylate (ACC), deaminase, plant growth-promoting rizobacteria, carnation, salinity stress.