African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Development of a PCR-based method for detection of Nosema pernyi

  Yi-Ren Jiang1,2, Zhen-Hua Deng2, Sheng-Lin Shi2, Rui-Sheng Yang2, Yan-Zhuo Li2, Yu-Xi Duan1* and Li Qin2* 
  1College of Plant Protection, Shenyang Agriculture University, Shenyang 110866, China.  2College of BioScience and Biotechnology, Shenyang Agriculture University, Shenyang 110866, China.
Email: [email protected] or [email protected]

  •  Accepted: 30 June 2011
  •  Published: 30 October 2011



Nosema pernyi is the lethal pathogen of pebrine disease in Antheraea pernyi. We have developed a PCR-based method for detection of N. pernyi using specific primers. The primers were designed by the reported conserved regions of microsporidian SSU rRNA. When the genomic DNA of N. pernyi was used as the DNA template, the specific DNA sequences were amplified by PCR. It was observed that PCR diagnosis of N. pernyi using the three sets of primers provided increased specificity and sensitivity when compared with light microscopy.


Key words: Nosema pernyi, Antheraea pernyi, pebrine disease, spores, PCR, detection.