The rate and optimum conditions for production of vitamin B12 and folic acid from a mixture of agricultural wastes, rice bran and wheat straw was studied with new bacterial isolates, using solid-state fermentation (SSF). The isolates Klebsiella pneumoniae andCitrobacter freundii showed the highest yields of 38.74 and 45.21 µg/l for vitamin B12 and folic acid, respectively, from 200 g/l of the studied wastes. The optimum fermentation temperature for this production was 30°C for 3 days. This method is important to exploit the agricultural wastes which cause severe problems for the environment.
Key words: Bacteria, folic acid, solid-state fermentation, vitamin B12
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