African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Feeding of L-phenylalanine as precursor enhances strobilurin A biosynthesis in the basidiomycte, Strobilurus tenacellus

Zafar Iqbal*, Mudassar Iqbal, Hamida Bibi, Zia ud Din, Muhammad Idrees, Muhammad Sajid, Ijaz Ahmad Khan and Hamid Ullah Shah
The University of Agriculture, Peshawar, Pakistan
Email: [email protected]

  •  Accepted: 08 January 2013
  •  Published: 12 March 2013

Abstract

In this study, the effect of different concentrations of L-phenylalanine (0, 0.10, 0.25, 0.50, 1 and 2 mM) was used as a precursor to enhance antifungal natural product strobilurin A, which was investigated in the fungus Strobilurus tenacellus. The putative precursor was added to the fungal shake flask culture and the concentration of strobilurin A was quantified by liquid chromatography and mass spectroscopy (LCMS). The concentrations of the feeding flasks were compared with the control culture under similar conditions of fermentation. Highest yield (2.5 times higher than control) was observed in the flasks fed with 1 mM of L-phenylalanine. This supports the hypothesis that L-phenylalanine is the putative precursor of strobilurin A and during biosynthesis probably, the aromatic part of the compound is derived from this precursor.

 

Key words: Strobilurin A, Strobilurus tenacellus, phenylalanine precursor.