African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Solubilization of tricalcium phosphate by temperature and salt tolerant Serratia marcescens NBRI1213 isolated from alkaline soils

  Lavania M.1 and Nautiyal C. S.2*
  1The Energy and Resources Institute, Indian Habitat Center, Lodhi Road, New Delhi 11003, India. 2Division of Plant Microbe Interactions, National Botanical Research Institute, Pratap Marg, Lucknow 226 001, India.
Email: [email protected]

  •  Accepted: 06 August 2013
  •  Published: 23 August 2013



A number of stress tolerant plant growth promoting phosphate solubilizing bacteria were isolated from soils collected from different geo-climatic zones of India. Maximum numbers of these bacteria were found in Aurawan soil, followed by National Botanical Garden, Mukhateshwar and Nainital. Among these bacteria, strain NBRI1213 showed maximum phosphate solubilization activity (984 µg/mL) and was identified as Serratia marcescensby 16S rRNA full gene sequencing. It showed tolerance to wide range of temperature (30 to 50°C), salt (0 - 5% NaCl) and pH (7 - 10). Salicylic acid was found to be present in addition to oxalic, citric acid and acetic acid in culture filtrate. In a micro plot study, inoculation of maize seeds with phosphate solubilizing S. marcescens NBRI1213 showed better response than control. It did not only improve the quality but increased the soluble phosphorus content and enhanced the phosphate-solubilizing microbial populations of the soil. Smarcescens NBRI1213 isolated from alkaline soil was found to be an efficient phosphate solublizer and can be used as potential plant growth promoting rhizobacteria bio-inoculant in agricultural systems.


Key words: Phosphate solubilization, Serratia marcescens, stress tolerance, rhizosphere competence, maize.