African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

The extraction on polysaccharide of sporocarp and static culture optimization conditions of Morchella esculenta from Qinghai-Tibetan Plateau

Ke-long Chen1, Xu Su1, 2*, Yu-ping Liu3, Heng-sheng Wang1, Zhao-meng Li1, Jian-min LI1 and Ben La1
1Key Laboratory of Education Ministry of Environments and Resources in Qinghai-Tibetan Plateau, School of Geography and Life Science, Qinghai Normal University, Xining 810008, China. 2State Key Laboratory Breeding Base-Key Laboratory of Qinghai Province for Plateau Crop Germplasm Innovation and Utilization, Qinghai University, Xining 810016, China. 3Department of Ideological and Political Theory Teaching and Research, Qinghai Normal University, Xining 810008, China.
Email: [email protected]

  •  Accepted: 20 September 2013
  •  Published: 11 October 2013


In this paper, adopting orthogonal design method, the Morchella esculenta mycelia from Qinghai-Tibetan plateau was preliminary studied in different growing conditions such as pH, temperature, carbon sources, nitrogen sources and growth factors. The results showed that the M. esculenta mycelium liquid fermentation pH was between 6.0 to 7.0, cultivating optimum temperature 24ËšC, training required for 1.2% of potassium nitrate nitrogen (KNO3), carbon source was 4.5% soluble starch, growth factor biotin was 0.1%. At the same time, we also took the plateau M. esculenta polysaccharide fermentation liquid extraction, the extraction time was optimal combination 2 h, precipitated with ethanol concentration of 90% extraction ratio 1:20, the highest yield of polysaccharides.


Key words: Qinghai-Tibetan Plateau, Morchella esculenta, sporocarp, mycelium, static culture.