African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Study on liquid paraffin decomposer microorganisms isolated from soils of Julfa-Iran

Haedeh Mobaiyen1*, Khosro Sedegbayan1, Alireza Dehnad2, Abdol-Hosen Naseri3,Farhad Ahmad por1, and Naser Moder Shahla4
1Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran. 2Department of Microbial Biotechnology, AREEO, Tabriz, Iran. 3Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Iran. 4Department of Analytical Chemistry, Faculty of Chemistry, Tabriz Branch, Islamic  Azad  University, Tabriz, Iran
Email: [email protected]

  •  Accepted: 23 January 2013
  •  Published: 31 January 2013


Paraffin compounds are derived from petroleum. Natural environment pollution with petroleum or petroleum products is still an environmental problem. The objective of the present study was to isolate the environmental liquid paraffin decomposer strains isolated from soil in Julfa, Iran. Initially, we collected soil samples from different areas and by preparation of serial dilution for 10-1 to 10-6 of them and culture in the Starch Casein Agar medium, 10  pure different colonies were isolated, from that, six strains in duplicate experiment showed paraffin decomposition ability about more than 70%. In the strains, resulted product was analyzed by gas chromatography (GC) mass test, as approval method of the paraffin decomposition. Then, for molecular recognition of isolates by specific primers of 16S rRNA, PCR were done and the resulted product of PCR was sequenced and considered strains were isolated. In this study, by sequencing of PCR products, the isolated strains were identified as Streptomyces lavendulaeBacillus sp.and Streptomyces paracox.


Key words: Liquid paraffin, degradation, microorganism, soil, petroleum, chromatography, Iran