Abstract

In this study, human-like collagen sponge (HLCS) was prepared by cross linking with 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC). Setting Double Diamondâ—‹R Collagen Sponge (DDCS) as the control group, arterioles and liver injury model was made to compare the hemostatic efficacy and biocompatibility of HLCS. In rabbit ear central artery injury hemostatic test, the complete hemostasis time of HLCS and DDCS was significantly different, which was 79 ± 8 s and 121 ± 17 s, respectively (P< 0.05). In rabbit liver injury hemostatic test, the complete hemostasis time of HLCS was 51 ± 6 s, and that of DDCS was 95 ± 12s, which was also significantly different (P < 0.05). The results of optical microscopy and transmission electron microscopy suggested that the damage of the hepatocyte from HLCS was much slighter. The results indicated that HLCS had excellent biocompatibility and it might be a potential material as a topical hemostatic agent.

Key words: Human-like collagen, sponge, hemostatic, biocompatibility.