African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5209

Full Length Research Paper

Effect of lipopolysaccharide on nitric oxide (NO) production in culture supernatants exposed to Bacillus Calmette-Guerin (BCG) and supplemented with 1, 25(OH)2D3

Aurora Martínez-Romero1, José Luis Ortega-Sánchez2, José Ramón Hernández-Salgado2, Norma Urtiz-Estrada3, Maribel Cervantes-Flores3, Estela Ruíz-Baca3 and José de Jesús Alba-Romero1*
1División de Estudios de Postgrado e Investigación. Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango. AP No. 51 Gómez Palacio, Durango. México. 2Unidad Regional Universitaria de Zonas Áridas, Universidad Autónoma Chapingo, AP No. 8 Bermejillo, Durango México. 3Laboratorio de Biología Celular y Molecular, Facultad de Ciencias Químicas, Unidad Durango. UJED, México.
Email: [email protected]

  •  Accepted: 26 August 2013
  •  Published: 07 November 2013


Recently, there has been a striking increase in the number of patients with mycobacterial disease, and this is an important health problem around the world. One essential component of tubercular host defense includes nitric oxide production. The objective of this study was to investigate in vitro the effect of lipopolysaccharide on nitric oxide production in supernatants of mononuclear cell cultures from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 Dihydroxyvitamin D3 [1,25(OH)2 D3].  Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero days), 3, 7, 14 and 21 days after applying the treatments. The mononuclear cells by the ficoll-hypaque method were obtained. The nitric oxide concentrations were measured directly by griess method with ELISA reader. The treatment with the 1,25(OH)2D3 stimulated the nitric oxide synthesis indicating, that by itself it is a good modulator of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)2D3. The exhibition to M. bovis-BCG vaccine with 1,25(OH)2D3 was able to increase answer NO2- in exposed animals. The LPS has effect in vitro in the production of nitric oxide in goats exposed to M. bovis BCG vaccine and supplemented with 1,25(OH)2D3.

Key words: Nitric oxide, lipopolysaccharide, M. bovis-BCG vaccine, 1, 25 Dihydroxyvitamin D3, goats.


TB, Tuberculosis; Mtb, Mycobacterium tuberculosis; TNF, tumor necrosis factor alpha; PBMC, peripheral blood mononuclear cells; BCG, bacillus Calmette-Guerin; GM-CSF, granulocyte-macrophage colony-stimulating factor; ROI, reactive oxygen intermediates; RNI, reactive nitrogen intermediates; LPS, lipopolysaccharide; MNCC, mononuclear cell cultures.