African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

DNA-binding activity and in vivo cytotoxicity of Ganoderma applanatum (Pers.) Pat. supercritical-CO2 extracts

Zeus S. Elumba1, Franco G. Teves2* and Roberto M. Malaluan3
1Department of Biology, Central Mindanao University, Musuan, Bukidnon, Philippines. 2Department of Biological Sciences, Mindanao State University-Iligan Institute of Technology, Iligan City, Philippines. 3Department of Chemical Engineering Technology, Mindanao State University-Iligan Institute of Technology, Iligan City, Philippines
Email: [email protected]

  •  Accepted: 11 October 2012
  •  Published: 31 January 2013


Ganoderma applanatum (Pers.) Pat. is a perennial and abundant mushroom in Bukidnon Province, Philippines but its bioactivities have just been investigated lately. In this study, three supercritical-CO2 (SC-CO2) extracts (GA1, GA2 and GA3) from the local strain ofG. applanatum (Pers.) Pat. were examined for their DNA-binding potential by means of biomolecular-chemical screening and in vivo cytotoxicity using brine shrimp lethality assay (BSLA). All extracts contained secondary metabolites that have DNA-binding activity towards salmon sperm DNA (Rf2/Rf1-ratio <1). For BSLA, the LC50 (24 h) values ranged from 177.82 to 354.81 µg/ml, indicating that the extracts are biologically active but with no significant cytotoxicity. Reverse-phase HPLC and FT-IR spectroscopy were carried out to partially characterize the extracts. Results of this study showed that G. applanatum (Pers.) Pat. from Bukidnon Province, Philippines is a potential source of biologically active, non-toxic compounds with DNA-binding activity.


Key words: Ganoderma applanatum, DNA-binding activity, cytotoxicity, supercritical-CO2