African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5211

Full Length Research Paper

Distribution of microbial communities in Guiyu soils and sediments investigated by 16S rRNA gene library and denaturing gradient gel electrophoresis (DGGE) fingerprinting

CHEN Biao#, LIN Bokun#, LUN Jingsheng, CHEN Pengcheng, HUANG Tongwang*,and HU Zhong*  
Department of Biology, College of Science, Shantou University, Shantou 515063, People’s Republic of China. #These authors contributed equally to the work.
Email: [email protected], [email protected]

  •  Accepted: 08 January 2013
  •  Published: 09 April 2013


Guiyu, a town in south China, seriously polluted by heavy metals and toxic organic compounds, takes an important part in the cycle of the Waste Electrical and Electronic Equipment. Here, culture-independent approaches were used to analyze the bacterial community structure of 7 soil and sediment samples. The objective of this paper was to examine the distribution of microbial communities in the e-waste contaminated areas of Guiyu. High concentrations of metals (Cu, Pb, Zn and Al) were detected in the soil and sediment samples collected from the e-waste contaminated areas at Guiyu. Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene library technology were used to investigate the microbial communities. The 16S rRNA gene library could detect more bacteria than polymerase chain reaction (PCR)-DGGE. The main bacteria in the soil and sediment samples were Deltaproteobacteria (36%), Firmicutes (16%),Alphaproteobacteria (9%), Bacteroidetes (7%), Verrucomicrobia (7%) and Acidobacteria(5%); and 20% of the sequences in the 16S rRNA gene library had sequence identities less than 92% with known sequences.


Key words: Guiyu, microbial diversity, DGGE, 16S rRNA gene library.